Restriction map for
pFN21AB8943
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaI
BseRI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
FseI FspI HincII HindII HpaI
KasI KpnI KspI MfeI MflI
MluI MluNI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsaI RsrII SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tsp509I Tth111I Van91I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 247 CGCG CG^CG
AccIII 6 TCCGGA T^CCGGA
AcyI 882 GACGCC G[GA]^CG[CT]C
AlwI 164 GGATC GGATC(4/5)
ApaI 122 GGGCCC GGGCC^C
BanI 729 GGTGCC G^G[CT][GA]CC
BbsI 754 GTCTTC GAAGAC(2/6)
BbvCI 565 GCTGAGG CCTCAGC(-5/-2)
BclI 416 TGATCA T^GATCA
BfaI 562 CTAG C^TAG
BglI 298 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 264 CTCCAG CTGGAG(16/14)
BpuAI 754 GTCTTC GAAGAC(2/6)
BsaBI 744 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 882 GACGCC G[GA]^CG[CT]C
BsaWI 6 TCCGGA [AT]^CCGG[AT]
BseAI 6 TCCGGA T^CCGGA
BsiEI 798 CGACCG CG[GA][CT]^CG
BspEI 6 TCCGGA T^CCGGA
BspHI 413 TCATGA T^CATGA
BspMI 424 ACCTGC ACCTGC(4/8)
BsrBI 774 CCGCTC CCGCTC(-3/-3)
BsrDI 307 GCAATG GCAATG(2/0)
BstAPI 204 GCACTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 247 CGCG CG^CG
DdeI 566 CTGAG C^T[ACGT]AG
DsaI 299 CCATGG C^C[GA][CT]GG
EaeI 168 CGGCCA [CT]^GGCC[GA]
EarI 556 CTCTTC CTCTTC(1/4)
EcoNI 561 CCTAGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1 ATGCAT ATGCA^T
FbaI 416 TGATCA T^GATCA
HaeII 216 GGCGCT [GA]GCGC^[CT]
Hin1I 882 GACGCC G[GA]^CG[CT]C
HindIII 253 AAGCTT A^AGCTT
HinfI 484 GAGTC G^A[ACGT]TC
Ksp632I 556 CTCTTC CTCTTC(1/4)
MaeI 562 CTAG C^TAG
MamI 744 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 6 TCCGGA T^CCGGA
MseI 257 TTAA T^TAA
MvnI 247 CGCG CG^CG
NcoI 299 CCATGG C^CATGG
NsiI 1 ATGCAT ATGCA^T
NspI 500 GCATGC [GA]CATG^[CT]
PleI 484 GAGTC GAGTC(4/5)
RcaI 413 TCATGA T^CATGA
SacI 814 GAGCTC GAGCT^C
SapI 555 GCTCTTC GCTCTTC(1/4)
SphI 500 GCATGC GCATG^C
Tru9I 257 TTAA T^TAA
Tsp45I 148 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 397 GTGCAC G^TGCAC
460 GTGCAC
ApaLI 397 GTGCAC G^TGCAC
460 GTGCAC
BsgI 375 CTGCAC GTGCAG(16/14)
319 GTGCAG
BstF5I 4 CATCC GGATG(2/0)
429 CATCC
BstXI 380 CCACAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
719 CCACCATCATGG
DraII 121 GGGGCCC [GA]G^G[ACGT]CC[CT]
122 GGGCCCC
EcoO109I 121 GGGGCCC [GA]G^G[ACGT]CC[CT]
122 GGGCCCC
EcoT14I 299 CCATGG C^C[AT][AT]GG
625 CCAAGG
FokI 4 CATCC GGATG(9/13)
429 CATCC
HgaI 328 GACGC GACGC(5/10)
882 GACGC
MaeII 458 ACGT A^CGT
668 ACGT
MaeIII 148 GTCAC ^GT[ACGT]AC
176 GTTAC
MboII 557 TCTTC GAAGA(8/7)
755 TCTTC
SmaI 117 CCCGGG CCC^GGG
690 CCCGGG
SmlI 240 CTTGAG C^T[CT][GA]AG
604 CTCAAG
StyI 299 CCATGG C^C[AT][AT]GG
625 CCAAGG
XcmI 299 CCATGGTGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
380 CCACAGTGCTGGTGG
XmaCI 117 CCCGGG C^CCGGG
690 CCCGGG
XmaI 117 CCCGGG C^CCGGG
690 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstNI
Cac8I CfoI Cfr13I DpnII EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PvuII Sau3AI Sau96I ScrFI SfaNI
TaqI TseI TspRI TthHB8I