Restriction map for
pFN21AE5020
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AvrII BalI
BamHI BanII BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BpuAI BsaI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HpaI KpnI MaeI MfeI
MluI MluNI MscI MunI NcoI
NdeI NheI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SfuI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 865 GACGTC GACGT^C
AccIII 137 TCCGGA T^CCGGA
AcsI 235 AAATTC [GA]^AATT[CT]
AflIII 458 ACGTGT A^C[GA][CT]GT
AhdI 874 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 235 AAATTC [GA]^AATT[CT]
AspEI 874 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 888 GTGCTC G[AT]GC[AT]^C
AspI 21 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 85 CTCGAG C^[CT]CG[GA]G
AviII 446 TGCGCA TGC^GCA
BcnI 870 CCCGG CC^[GC]GG
BglI 840 GCCCAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1007 AGATCT A^GATCT
BpmI 184 CTGGAG CTGGAG(16/14)
BsaBI 135 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 137 TCCGGA [AT]^CCGG[AT]
BseAI 137 TCCGGA T^CCGGA
BsiHKAI 888 GTGCTC G[AT]GC[AT]^C
BsoBI 85 CTCGAG C^[CT]CG[GA]G
BspEI 137 TCCGGA T^CCGGA
BssSI 97 CACGAG CACGAG(-5/-1)
BstAPI 9 GCAGAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 706 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 835 CCGCGG C^C[GA][CT]GG
Eam1105I 874 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 706 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 629 CCAAGG C^C[AT][AT]GG
FspI 446 TGCGCA TGC^GCA
HincII 588 GTTGAC GT[CT]^[GA]AC
HindII 588 GTTGAC GT[CT]^[GA]AC
HindIII 961 AAGCTT A^AGCTT
KspI 835 CCGCGG CCGC^GG
MamI 135 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 137 TCCGGA T^CCGGA
NciI 870 CCCGG CC^[GC]GG
PaeR7I 85 CTCGAG C^TCGAG
PflFI 21 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 375 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 360 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 181 CAGCTG CAG^CTG
SacII 835 CCGCGG CCGC^GG
SexAI 719 ACCTGGT A^CC[AT]GGT
SfiI 839 GGCCCAGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 219 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 85 CTCGAG C^T[CT][GA]AG
SphI 443 GCATGC GCATG^C
StuI 320 AGGCCT AGG^CCT
StyI 629 CCAAGG C^C[AT][AT]GG
Tsp45I 478 GTGAC ^GT[GC]AC
Tsp509I 236 AATT ^AATT
Tth111I 21 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 375 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 85 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 153 GTAC GT^AC
855 GTAC
AlwNI 376 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1093 CAGAGGCTG
AocI 729 CCTCAGG CC^T[ACGT]AGG
826 CCTGAGG
Asp700I 396 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
945 GAACTCCTTC
AvaII 93 GGACC G^G[AT]CC
873 GGACC
BbeI 64 GGCGCC GGCGC^C
634 GGCGCC
BmyI 289 GGGCAC G[AGT]GC[ACT]^C
888 GTGCTC
BsaAI 613 TACGTG [CT]AC^GT[GA]
658 TACGTG
BsiEI 524 CGGCCG CG[GA][CT]^CG
924 CGGCCG
Bsp1286I 289 GGGCAC G[AGT]GC[ACT]^C
888 GTGCTC
BstYI 134 AGATCC [GA]^GATC[CT]
1007 AGATCT
Bsu36I 729 CCTCAGG CC^T[ACGT]AGG
826 CCTGAGG
DdeI 730 CTCAG C^T[ACGT]AG
827 CTGAG
EaeI 524 CGGCCG [CT]^GGCC[GA]
924 CGGCCG
EagI 524 CGGCCG C^GGCCG
924 CGGCCG
EclXI 524 CGGCCG C^GGCCG
924 CGGCCG
Eco52I 524 CGGCCG C^GGCCG
924 CGGCCG
Eco81I 729 CCTCAGG CC^T[ACGT]AGG
826 CCTGAGG
HinfI 21 GACTC G^A[ACGT]TC
88 GAGTC
KasI 64 GGCGCC G^GCGCC
634 GGCGCC
MaeIII 478 GTGAC ^GT[ACGT]AC
1071 GTTAC
MflI 134 AGATCC [GA]^GATC[CT]
1007 AGATCT
MseI 428 TTAA T^TAA
1024 TTAA
MslI 399 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
453 CACTGACGTG
NarI 64 GGCGCC GG^CGCC
634 GGCGCC
NotI 523 GCGGCCGC GC^GGCCGC
923 GCGGCCGC
NspI 329 GCATGT [GA]CATG^[CT]
443 GCATGC
PleI 21 GACTC GAGTC(4/5)
88 GAGTC
RsaI 153 GTAC GT^AC
855 GTAC
SfoI 64 GGCGCC GGC^GCC
634 GGCGCC
Tru9I 428 TTAA T^TAA
1024 TTAA
XhoII 134 AGATCC [GA]^GATC[CT]
1007 AGATCT
XmnI 396 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
945 GAACTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
BanI BbvI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsmFI BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DpnII EarI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NdeII NgoMIV
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
TspRI TthHB8I