Restriction map for
pFN21AE5653
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwI Aor51HI ApaI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmFI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstBI
BstUI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HpaI HpaII KasI KspI
MaeII MamI MflI MluI MluNI
MroI MscI MslI MspI MvnI
NaeI NarI NciI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tth111I XbaI XcmI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 231 GGTACC G^GTACC
AciI 545 CCGC CCGC(-3/-1)
AlwNI 1091 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 694 CCTCAGG CC^T[ACGT]AGG
Asp718I 231 GGTACC G^GTACC
AvaI 557 CTCGAG C^[CT]CG[GA]G
AvaII 899 GGTCC G^G[AT]CC
BanI 231 GGTACC G^G[CT][GA]CC
BbsI 775 GAAGAC GAAGAC(2/6)
BpuAI 775 GAAGAC GAAGAC(2/6)
BsaI 1201 GAGACC GGTCTC(1/5)
BsoBI 557 CTCGAG C^[CT]CG[GA]G
BspMI 118 ACCTGC ACCTGC(4/8)
BsrDI 1129 GCAATG GCAATG(2/0)
BssSI 1277 CACGAG CACGAG(-5/-1)
BstAPI 113 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 562 GGATG GGATG(2/0)
BstXI 213 CCAGTTAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 694 CCTCAGG CC^T[ACGT]AGG
DpnII 238 GATC ^GATC
DraIII 332 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 694 CCTCAGG CC^T[ACGT]AGG
FokI 562 GGATG GGATG(9/13)
HgaI 1093 GACGC GACGC(5/10)
HindIII 143 AAGCTT A^AGCTT
KpnI 231 GGTACC GGTAC^C
MboI 238 GATC ^GATC
MfeI 1004 CAATTG C^AATTG
MunI 1004 CAATTG C^AATTG
NcoI 863 CCATGG C^CATGG
NdeII 238 GATC ^GATC
PaeR7I 557 CTCGAG C^TCGAG
SacI 1399 GAGCTC GAGCT^C
SapI 1312 GAAGAGC GCTCTTC(1/4)
Sau3AI 238 GATC ^GATC
SfcI 961 CTACAG C^T[GA][CT]AG
XhoI 557 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
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Alw44I 73 GTGCAC G^TGCAC
371 GTGCAC
ApaLI 73 GTGCAC G^TGCAC
371 GTGCAC
BpmI 211 CTCCAG CTGGAG(16/14)
968 CTCCAG
Bsp1407I 1291 TGTACA T^GTACA
1389 TGTACA
BsrGI 1291 TGTACA T^GTACA
1389 TGTACA
BssKI 228 CCAGG ^CC[ACGT]GG
993 CCAGG
BstEII 759 GGTTACC G^GT[ACGT]ACC
1024 GGTGACC
BstNI 228 CCAGG CC^[AT]GG
993 CCAGG
BstPI 759 GGTTACC G^GT[ACGT]ACC
1024 GGTGACC
DsaI 863 CCATGG C^C[GA][CT]GG
1335 CCGTGG
EcoO65I 759 GGTTACC G^GT[ACGT]ACC
1024 GGTGACC
EcoRII 228 CCAGG ^CC[AT]GG
993 CCAGG
EcoT14I 471 CCTTGG C^C[AT][AT]GG
863 CCATGG
HincII 196 GTCAAC GT[CT]^[GA]AC
419 GTCAAC
HindII 196 GTCAAC GT[CT]^[GA]AC
419 GTCAAC
MvaI 228 CCAGG CC^[AT]GG
993 CCAGG
NheI 355 GCTAGC G^CTAGC
1375 GCTAGC
NlaIV 223 GGAGCC GG[ACGT]^[ACGT]CC
231 GGTACC
PflMI 331 CCACTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
533 CCAATCTATGG
PvuII 793 CAGCTG CAG^CTG
1117 CAGCTG
ScrFI 228 CCAGG CC^[ACGT]GG
993 CCAGG
SmlI 557 CTCGAG C^T[CT][GA]AG
642 CTCAAG
SspBI 1291 TGTACA T^GTACA
1389 TGTACA
StuI 596 AGGCCT AGG^CCT
1051 AGGCCT
StyI 471 CCTTGG C^C[AT][AT]GG
863 CCATGG
Tsp45I 403 GTGAC ^GT[GC]AC
1025 GTGAC
Van91I 331 CCACTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
533 CCAATCTATGG
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
BanII BbvCI BbvI BfaI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI Cac8I Cfr13I
DdeI EarI Fnu4HI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboII MnlI MseI MspA1I MwoI
NlaIII NspI PleI RsaI Sau96I
SfaNI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I