Restriction map for
pFN21AE5008
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI Alw44I AlwI
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsiEI
BsiHKAI BsiWI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MaeIII
MamI MluI MluNI MscI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 10 TCCGGA T^CCGGA
AcsI 112 GAATTC [GA]^AATT[CT]
AfaI 834 GTAC GT^AC
AhdI 187 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 419 AGCGCT AGC^GCT
ApoI 112 GAATTC [GA]^AATT[CT]
Asp700I 490 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 187 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 627 GGGCTC G[GA]GC[CT]^C
BbsI 406 GAAGAC GAAGAC(2/6)
BcgI 438 CGAAAGAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 406 GAAGAC GAAGAC(2/6)
BsaWI 10 TCCGGA [AT]^CCGG[AT]
BseAI 10 TCCGGA T^CCGGA
BseRI 31 GAGGAG GAGGAG(10/8)
BspEI 10 TCCGGA T^CCGGA
BspMI 738 ACCTGC ACCTGC(4/8)
BsrDI 648 CATTGC GCAATG(2/0)
BsrFI 269 ACCGGC [GA]^CCGG[CT]
BssHII 384 GCGCGC G^CGCGC
BstXI 581 CCAGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 269 ACCGGC [GA]^CCGG[CT]
DsaI 623 CCACGG C^C[GA][CT]GG
Eam1105I 187 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 419 AGCGCT AGC^GCT
EcoRI 112 GAATTC G^AATTC
EcoRV 750 GATATC GAT^ATC
EcoT14I 284 CCTTGG C^C[AT][AT]GG
FauI 899 GCGGG CCCGC(4/6)
HaeII 419 AGCGCT [GA]GCGC^[CT]
HgaI 153 GACGC GACGC(5/10)
MaeII 699 ACGT A^CGT
MfeI 325 CAATTG C^AATTG
MroI 10 TCCGGA T^CCGGA
MseI 317 TTAA T^TAA
MunI 325 CAATTG C^AATTG
PflMI 865 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 834 GTAC GT^AC
SexAI 985 ACCAGGT A^CC[AT]GGT
SmlI 669 CTCAAG C^T[CT][GA]AG
StyI 284 CCTTGG C^C[AT][AT]GG
Tru9I 317 TTAA T^TAA
Van91I 865 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 923 CCAGCGTGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 490 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 300 CGCG CG^CG
385 CGCG
BcnI 719 CCGGG CC^[GC]GG
746 CCCGG
BglII 57 AGATCT A^GATCT
275 AGATCT
BmyI 627 GGGCTC G[AGT]GC[ACT]^C
794 GGGCAC
BpmI 349 CTGGAG CTGGAG(16/14)
799 CTGGAG
BsaI 185 GAGACC GGTCTC(1/5)
773 GAGACC
BsmBI 151 GAGACG CGTCTC(1/5)
906 GAGACG
BsmFI 724 GGGAC GGGAC(10/14)
843 GTCCC
Bsp1286I 627 GGGCTC G[AGT]GC[ACT]^C
794 GGGCAC
BsrI 537 ACTGG ACTGG(1/-1)
798 ACTGG
BssSI 954 CACGAG CACGAG(-5/-1)
829 CTCGTG
BstF5I 291 GGATG GGATG(2/0)
510 GGATG
BstUI 300 CGCG CG^CG
385 CGCG
BstYI 57 AGATCT [GA]^GATC[CT]
275 AGATCT
EaeI 539 TGGCCG [CT]^GGCC[GA]
910 CGGCCA
FokI 291 GGATG GGATG(9/13)
510 GGATG
HphI 937 GGTGA GGTGA(8/7)
654 TCACC
MboII 37 GAAGA GAAGA(8/7)
406 GAAGA
MflI 57 AGATCT [GA]^GATC[CT]
275 AGATCT
MvnI 300 CGCG CG^CG
385 CGCG
NciI 719 CCGGG CC^[GC]GG
746 CCCGG
PvuII 157 CAGCTG CAG^CTG
611 CAGCTG
TaqI 404 TCGA T^CGA
437 TCGA
TspRI 587 CAGTG CA[GC]TG[ACGT][ACGT]^
797 CACTG
TthHB8I 404 TCGA T^CGA
437 TCGA
XhoII 57 AGATCT [GA]^GATC[CT]
275 AGATCT
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BsaJI BsgI BsiYI BslI BsmAI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboI MnlI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
Sau3AI Sau96I ScrFI TfiI TseI
Tsp509I