Restriction map for
pFN21AE5007
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TfiI Tth111I Van91I
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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Aor51HI 56 AGCGCT AGC^GCT
ApaI 213 GGGCCC GGGCC^C
AvaI 209 CCCGGG C^[CT]CG[GA]G
BbsI 602 GAAGAC GAAGAC(2/6)
BbvI 632 GCAGC GCAGC(8/12)
BciVI 268 GGATAC GTATCC(6/5)
BpuAI 602 GAAGAC GAAGAC(2/6)
BseRI 82 CTCCTC GAGGAG(10/8)
BsgI 360 GTGCAG GTGCAG(16/14)
BsiEI 225 CGATCG CG[GA][CT]^CG
BsoBI 209 CCCGGG C^[CT]CG[GA]G
DraI 375 TTTAAA TTT^AAA
DraII 212 GGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 75 TGGCCG [CT]^GGCC[GA]
Eco47III 56 AGCGCT AGC^GCT
EcoO109I 212 GGGGCCC [GA]G^G[ACGT]CC[CT]
HaeII 56 AGCGCT [GA]GCGC^[CT]
MseI 376 TTAA T^TAA
NlaIII 474 CATG CATG^
NruI 222 TCGCGA TCG^CGA
PvuI 225 CGATCG CGAT^CG
SacI 175 GAGCTC GAGCT^C
SfaNI 418 GATGC GCATC(5/9)
SfcI 237 CTACAG C^T[GA][CT]AG
SmaI 209 CCCGGG CCC^GGG
SspI 523 AATATT AAT^ATT
Tru9I 376 TTAA T^TAA
TseI 632 GCAGC G^C[AT]GC
XmaCI 209 CCCGGG C^CCGGG
XmaI 209 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 10 GTCTAC GT^[AC][GT]AC
91 GTAGAC
AfaI 108 GTAC GT^AC
320 GTAC
AspHI 175 GAGCTC G[AT]GC[AT]^C
202 GAGCAC
BsaI 590 GGTCTC GGTCTC(1/5)
824 GGTCTC
BsaJI 209 CCCGGG C^C[ACGT][ACGT]GG
210 CCGGGG
BsiHKAI 175 GAGCTC G[AT]GC[AT]^C
202 GAGCAC
BspMI 397 GCAGGT ACCTGC(4/8)
821 GCAGGT
BsrI 495 ACTGG ACTGG(1/-1)
891 CCAGT
FauI 134 CCCGC CCCGC(4/6)
216 CCCGC
HapII 39 CCGG C^CGG
210 CCGG
HinfI 130 GACTC G^A[ACGT]TC
621 GAGTC
HpaII 39 CCGG C^CGG
210 CCGG
HphI 400 GGTGA GGTGA(8/7)
859 GGTGA
MaeII 35 ACGT A^CGT
702 ACGT
MboII 538 GAAGA GAAGA(8/7)
602 GAAGA
MspI 39 CCGG C^CGG
210 CCGG
PleI 130 GACTC GAGTC(4/5)
621 GAGTC
RsaI 108 GTAC GT^AC
320 GTAC
Tsp45I 230 GTGAC ^GT[GC]AC
609 GTCAC
Tsp509I 450 AATT ^AATT
904 AATT
TspRI 66 CAGTG CA[GC]TG[ACGT][ACGT]^
255 CAGTG
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaII BanII BcnI BfaI BglII
BmyI BsaHI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HgaI HhaI Hin1I HinP1I
ItaI MaeI MaeIII MboI MflI
MnlI MvaI MvnI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
TaqI TthHB8I XbaI XhoII