Restriction map for
pFN21AE5005
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AccI AccII AccIII AclI AflII
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AviII BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrI BlpI
Bpu1102I BpuAI BsaBI BsaI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
CelII ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoRV FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PmeI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tru9I Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 997 GACGTC GACGT^C
Acc65I 179 GGTACC G^GTACC
AcyI 997 GACGTC G[GA]^CG[CT]C
AgeI 209 ACCGGT A^CCGGT
ApaI 1102 GGGCCC GGGCC^C
Asp718I 179 GGTACC G^GTACC
AvaI 366 CTCGAG C^[CT]CG[GA]G
AvrII 1098 CCTAGG C^CTAGG
BalI 935 TGGCCA TGG^CCA
BanI 179 GGTACC G^G[CT][GA]CC
BbrPI 1255 CACGTG CAC^GTG
BglI 437 GCCGGATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 136 AGATCT A^GATCT
BlnI 1098 CCTAGG C^CTAGG
BsaAI 1255 CACGTG [CT]AC^GT[GA]
BsaHI 997 GACGTC G[GA]^CG[CT]C
BsiEI 1191 CGGCCG CG[GA][CT]^CG
BsmFI 111 GTCCC GGGAC(10/14)
BsmI 1016 GCATTC GAATGC(1/-1)
BsoBI 366 CTCGAG C^[CT]CG[GA]G
BstAPI 1007 GCATTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 424 GGTCACC G^GT[ACGT]ACC
BstPI 424 GGTCACC G^GT[ACGT]ACC
BstXI 926 CCACCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 615 CCACGG C^C[GA][CT]GG
EagI 1191 CGGCCG C^GGCCG
EclXI 1191 CGGCCG C^GGCCG
Eco52I 1191 CGGCCG C^GGCCG
EcoNI 167 CCTACCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 424 GGTCACC G^GT[ACGT]ACC
EcoRI 807 GAATTC G^AATTC
EcoT14I 1098 CCTAGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
Hin1I 997 GACGTC G[GA]^CG[CT]C
KpnI 179 GGTACC GGTAC^C
MluNI 935 TGGCCA TGG^CCA
MscI 935 TGGCCA TGG^CCA
NsiI 1 ATGCAT ATGCA^T
PaeR7I 366 CTCGAG C^TCGAG
PflMI 229 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 209 ACCGGT A^CCGGT
PmaCI 1255 CACGTG CAC^GTG
PmlI 1255 CACGTG CAC^GTG
PpuMI 70 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 640 GAGCTC GAGCT^C
SapI 217 GAAGAGC GCTCTTC(1/4)
ScaI 1028 AGTACT AGT^ACT
SfcI 75 CTACAG C^T[GA][CT]AG
SphI 315 GCATGC GCATG^C
SspI 1145 AATATT AAT^ATT
StyI 1098 CCTAGG C^C[AT][AT]GG
TaqI 367 TCGA T^CGA
TfiI 759 GAATC G^A[AT]TC
TthHB8I 367 TCGA T^CGA
Van91I 229 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 872 CCAGCATCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 366 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 807 GAATTC [GA]^AATT[CT]
1291 GAATTT
AflIII 623 ACATGT A^C[GA][CT]GT
705 ACATGT
AocI 958 CCTGAGG CC^T[ACGT]AGG
1360 CCTGAGG
ApoI 807 GAATTC [GA]^AATT[CT]
1291 GAATTT
Asp700I 678 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1143 GAAATATTTC
BpmI 1002 CTGGAG CTGGAG(16/14)
1249 CTGGAG
BsaWI 209 ACCGGT [AT]^CCGG[AT]
302 ACCGGA
BsgI 1333 CTGCAC GTGCAG(16/14)
1258 GTGCAG
BsmAI 96 GAGAC GTCTC(1/5)
471 GTCTC
BspLU11I 623 ACATGT A^CATGT
705 ACATGT
BsrFI 209 ACCGGT [GA]^CCGG[CT]
1193 GCCGGT
BsrI 602 ACTGG ACTGG(1/-1)
1317 ACTGG
BstYI 136 AGATCT [GA]^GATC[CT]
1094 AGATCC
Bsu36I 958 CCTGAGG CC^T[ACGT]AGG
1360 CCTGAGG
Cfr10I 209 ACCGGT [GA]^CCGG[CT]
1193 GCCGGT
DraIII 427 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1075 CACCTGGTG
Eco81I 958 CCTGAGG CC^T[ACGT]AGG
1360 CCTGAGG
FauI 308 CCCGC CCCGC(4/6)
954 CCCGC
HaeII 188 AGCGCC [GA]GCGC^[CT]
358 AGCGCC
MflI 136 AGATCT [GA]^GATC[CT]
1094 AGATCC
MspA1I 763 CAGCTG C[AC]G^C[GT]G
1261 CAGCTG
PleI 1243 GACTC GAGTC(4/5)
469 GAGTC
PvuII 763 CAGCTG CAG^CTG
1261 CAGCTG
SmlI 366 CTCGAG C^T[CT][GA]AG
970 CTTGAG
Tsp509I 808 AATT ^AATT
1292 AATT
XhoII 136 AGATCT [GA]^GATC[CT]
1094 AGATCC
XmnI 678 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1143 GAAATATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BcnI BfaI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BsrBI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI TseI Tsp45I
TspRI