Restriction map for
pFN21AE5002
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspHI AspI AvaI
AviII AvrII BamHI BanII BbrPI
BbvCI BcgI BclI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
FauI FbaI FseI FspI HpaI
KspI MfeI MluI MroI MunI
NdeI NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 926 GGTACC G^GTACC
AccI 563 GTCTAC GT^[AC][GT]AC
AccII 229 CGCG CG^CG
AclI 274 AACGTT AA^CGTT
AflII 549 CTTAAG C^TTAAG
AocI 135 CCTCAGG CC^T[ACGT]AGG
Asp718I 926 GGTACC G^GTACC
BalI 841 TGGCCA TGG^CCA
BbeI 39 GGCGCC GGCGC^C
BciVI 224 GGATAC GTATCC(6/5)
BcnI 612 CCGGG CC^[GC]GG
BfrI 549 CTTAAG C^TTAAG
BsaBI 1191 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 9 TCCGGT [AT]^CCGG[AT]
BspHI 1007 TCATGA T^CATGA
BsrFI 36 GCCGGC [GA]^CCGG[CT]
BssSI 1045 CACGAG CACGAG(-5/-1)
BstBI 154 TTCGAA TT^CGAA
BstEII 615 GGTAACC G^GT[ACGT]ACC
BstPI 615 GGTAACC G^GT[ACGT]ACC
BstUI 229 CGCG CG^CG
BstYI 1190 AGATCC [GA]^GATC[CT]
Bsu36I 135 CCTCAGG CC^T[ACGT]AGG
Cfr10I 36 GCCGGC [GA]^CCGG[CT]
DsaI 1823 CCATGG C^C[GA][CT]GG
EaeI 841 TGGCCA [CT]^GGCC[GA]
Eco81I 135 CCTCAGG CC^T[ACGT]AGG
EcoO65I 615 GGTAACC G^GT[ACGT]ACC
EcoRV 1375 GATATC GAT^ATC
EcoT22I 588 ATGCAT ATGCA^T
HaeII 39 GGCGCC [GA]GCGC^[CT]
HincII 795 GTCAAC GT[CT]^[GA]AC
HindII 795 GTCAAC GT[CT]^[GA]AC
HindIII 1095 AAGCTT A^AGCTT
KasI 39 GGCGCC G^GCGCC
KpnI 926 GGTACC GGTAC^C
MamI 1191 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1190 AGATCC [GA]^GATC[CT]
MluNI 841 TGGCCA TGG^CCA
MscI 841 TGGCCA TGG^CCA
MslI 1596 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1483 CAGCTG C[AC]G^C[GT]G
MvnI 229 CGCG CG^CG
NaeI 36 GCCGGC GCC^GGC
NarI 39 GGCGCC GG^CGCC
NciI 612 CCGGG CC^[GC]GG
NcoI 1823 CCATGG C^CATGG
NgoMIV 36 GCCGGC G^CCGGC
NsiI 588 ATGCAT ATGCA^T
NspV 154 TTCGAA TT^CGAA
PpuMI 899 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 274 AACGTT AA^CGTT
PvuII 1483 CAGCTG CAG^CTG
RcaI 1007 TCATGA T^CATGA
SfoI 39 GGCGCC GGC^GCC
SfuI 154 TTCGAA TT^CGAA
SmlI 549 CTTAAG C^T[CT][GA]AG
Tsp45I 13 GTGAC ^GT[GC]AC
XhoII 1190 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwI 1191 GATCC GGATC(4/5)
372 GGATC
BanI 39 GGCGCC G^G[CT][GA]CC
926 GGTACC
BsiYI 917 CCAGTGATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1194 CCGAATCCTGG
BslI 917 CCAGTGATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1194 CCGAATCCTGG
BsmBI 63 CGTCTC CGTCTC(1/5)
97 GAGACG
BspLU11I 1242 ACATGT A^CATGT
1849 ACATGT
BstF5I 1864 CATCC GGATG(2/0)
1001 GGATG
DraII 139 AGGCCCT [GA]G^G[ACGT]CC[CT]
899 GGGACCC
EarI 157 GAAGAG CTCTTC(1/4)
163 GAAGAG
EcoO109I 139 AGGCCCT [GA]G^G[ACGT]CC[CT]
899 GGGACCC
FokI 1864 CATCC GGATG(9/13)
1001 GGATG
Ksp632I 157 GAAGAG CTCTTC(1/4)
163 GAAGAG
PleI 666 GACTC GAGTC(4/5)
249 GAGTC
TaqI 155 TCGA T^CGA
375 TCGA
TthHB8I 155 TCGA T^CGA
375 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwNI ApoI Asp700I AvaII
BbsI BbvI BfaI BpuAI BsaHI
BsaJI BseRI BsmAI BsmFI BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp509I TspRI XmnI