Restriction map for
pFN21AE2797
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# NO CUT ENZYMES ( 91 enzymes) #
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Acc65I AclI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BanII BbrPI BcgI BclI
BfrI BglII BlpI Bpu1102I BsaAI
BsaBI BsiEI BsmI BspDI BssHII
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRV FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MluI MunI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 718 GACGTC GACGT^C
AccI 1222 GTCTAC GT^[AC][GT]AC
AcsI 2353 GAATTC [GA]^AATT[CT]
AflIII 1316 ACATGT A^C[GA][CT]GT
AlwNI 835 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 2353 GAATTC [GA]^AATT[CT]
AvrII 1452 CCTAGG C^CTAGG
BamHI 563 GGATCC G^GATCC
BbvCI 2130 GCTGAGG CCTCAGC(-5/-2)
BciVI 1947 GTATCC GTATCC(6/5)
BfaI 1453 CTAG C^TAG
BglI 733 GCCAAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1452 CCTAGG C^CTAGG
BsiWI 1142 CGTACG C^GTACG
Bsp1407I 1721 TGTACA T^GTACA
BspHI 1730 TCATGA T^CATGA
BspLU11I 1316 ACATGT A^CATGT
BsrBI 670 GAGCGG CCGCTC(-3/-3)
BsrGI 1721 TGTACA T^GTACA
BstEII 909 GGTGACC G^GT[ACGT]ACC
BstPI 909 GGTGACC G^GT[ACGT]ACC
EcoNI 513 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 909 GGTGACC G^GT[ACGT]ACC
EcoRI 2353 GAATTC G^AATTC
EcoT22I 2333 ATGCAT ATGCA^T
HincII 721 GTCAAC GT[CT]^[GA]AC
HindII 721 GTCAAC GT[CT]^[GA]AC
MaeI 1453 CTAG C^TAG
NdeI 1288 CATATG CA^TATG
NsiI 2333 ATGCAT ATGCA^T
PflMI 1707 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1730 TCATGA T^CATGA
SapI 328 GAAGAGC GCTCTTC(1/4)
SgrAI 708 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 266 CTCAAG C^T[CT][GA]AG
SphI 808 GCATGC GCATG^C
SspBI 1721 TGTACA T^GTACA
Tsp509I 2354 AATT ^AATT
Van91I 1707 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccIII 1109 TCCGGA T^CCGGA
2144 TCCGGA
Alw44I 119 GTGCAC G^TGCAC
311 GTGCAC
ApaLI 119 GTGCAC G^TGCAC
311 GTGCAC
AvaII 1147 GGACC G^G[AT]CC
2093 GGACC
BalI 320 TGGCCA TGG^CCA
1658 TGGCCA
BsaWI 1109 TCCGGA [AT]^CCGG[AT]
2144 TCCGGA
BseAI 1109 TCCGGA T^CCGGA
2144 TCCGGA
BsgI 1273 CTGCAC GTGCAG(16/14)
832 GTGCAG
BspEI 1109 TCCGGA T^CCGGA
2144 TCCGGA
BspMI 508 ACCTGC ACCTGC(4/8)
602 GCAGGT
BsrDI 1506 CATTGC GCAATG(2/0)
2019 CATTGC
BstAPI 1248 GCATGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1275 GCACACGCTGC
DraII 739 GGGGCCT [GA]G^G[ACGT]CC[CT]
2259 GGGGCCT
EcoO109I 739 GGGGCCT [GA]G^G[ACGT]CC[CT]
2259 GGGGCCT
MluNI 320 TGGCCA TGG^CCA
1658 TGGCCA
MroI 1109 TCCGGA T^CCGGA
2144 TCCGGA
MscI 320 TGGCCA TGG^CCA
1658 TGGCCA
MseI 529 TTAA T^TAA
2246 TTAA
NaeI 90 GCCGGC GCC^GGC
709 GCCGGC
NgoMIV 90 GCCGGC G^CCGGC
709 GCCGGC
PleI 250 GACTC GAGTC(4/5)
877 GACTC
SmaI 1388 CCCGGG CCC^GGG
2256 CCCGGG
Tru9I 529 TTAA T^TAA
2246 TTAA
Tsp45I 277 GTGAC ^GT[GC]AC
910 GTGAC
XcmI 308 CCAGTGCACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2090 CCAGGACCATCTTGG
XmaCI 1388 CCCGGG C^CCGGG
2256 CCCGGG
XmaI 1388 CCCGGG C^CCGGG
2256 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI BanI BbeI
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI StuI StyI
TaqI TfiI TseI TspRI TthHB8I
XhoII