Restriction map for
pFN21AE3874
##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII BamHI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlpI Bpu1102I BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HindIII KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuII RsrII SacII SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1897 GTCGAC GT^[AC][GT]AC
AgeI 1607 ACCGGT A^CCGGT
AlwNI 794 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 926 CCTCAGG CC^T[ACGT]AGG
AspI 628 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1378 CCTAGG C^CTAGG
BanI 1108 GGCGCC G^G[CT][GA]CC
BbeI 1108 GGCGCC GGCGC^C
BclI 1515 TGATCA T^GATCA
BlnI 1378 CCTAGG C^CTAGG
BsaAI 891 CACGTA [CT]AC^GT[GA]
BsmBI 1657 GAGACG CGTCTC(1/5)
BstXI 1416 CCAGGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1327 AGATCC [GA]^GATC[CT]
Bsu36I 926 CCTCAGG CC^T[ACGT]AGG
DsaI 48 CCACGG C^C[GA][CT]GG
Eco81I 926 CCTCAGG CC^T[ACGT]AGG
FbaI 1515 TGATCA T^GATCA
HaeII 1108 GGCGCC [GA]GCGC^[CT]
HpaI 1465 GTTAAC GTT^AAC
KasI 1108 GGCGCC G^GCGCC
MflI 1327 AGATCC [GA]^GATC[CT]
MslI 514 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1353 CAGCGG C[AC]G^C[GT]G
NarI 1108 GGCGCC GG^CGCC
NspI 668 ACATGC [GA]CATG^[CT]
PaeR7I 203 CTCGAG C^TCGAG
PflFI 628 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1811 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1607 ACCGGT A^CCGGT
SacI 695 GAGCTC GAGCT^C
SalI 1897 GTCGAC G^TCGAC
ScaI 227 AGTACT AGT^ACT
SfoI 1108 GGCGCC GGC^GCC
SmaI 209 CCCGGG CCC^GGG
SmlI 203 CTCGAG C^T[CT][GA]AG
SspI 320 AATATT AAT^ATT
Tth111I 628 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1811 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 203 CTCGAG C^TCGAG
XhoII 1327 AGATCC [GA]^GATC[CT]
XmaCI 209 CCCGGG C^CCGGG
XmaI 209 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 1108 GGCGCC G[GA]^CG[CT]C
1760 GACGCC
AfaI 228 GTAC GT^AC
1493 GTAC
ApaI 133 GGGCCC GGGCC^C
688 GGGCCC
BbsI 2042 GAAGAC GAAGAC(2/6)
1804 GTCTTC
BpuAI 2042 GAAGAC GAAGAC(2/6)
1804 GTCTTC
BsaHI 1108 GGCGCC G[GA]^CG[CT]C
1760 GACGCC
BsaI 157 GAGACC GGTCTC(1/5)
237 GAGACC
BsaWI 563 TCCGGT [AT]^CCGG[AT]
1607 ACCGGT
BseRI 409 CTCCTC GAGGAG(10/8)
412 CTCCTC
BsgI 813 CTGCAC GTGCAG(16/14)
2071 GTGCAG
BsmFI 973 GGGAC GGGAC(10/14)
567 GTCCC
BsrBI 1152 CCGCTC CCGCTC(-3/-3)
304 GAGCGG
BsrI 1073 ACTGG ACTGG(1/-1)
1423 ACTGG
BstAPI 224 GCAAGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1435 GCAGATGATGC
EcoNI 922 CCTCCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1931 CCTGTCCGAGG
Hin1I 1108 GGCGCC G[GA]^CG[CT]C
1760 GACGCC
HincII 1465 GTTAAC GT[CT]^[GA]AC
1897 GTCGAC
HindII 1465 GTTAAC GT[CT]^[GA]AC
1897 GTCGAC
PpuMI 808 AGGTCCT [GA]G^G[AT]CC[CT]
1010 AGGACCT
PstI 121 CTGCAG CTGCA^G
1578 CTGCAG
PvuI 184 CGATCG CGAT^CG
1661 CGATCG
RsaI 228 GTAC GT^AC
1493 GTAC
SapI 1077 GAAGAGC GCTCTTC(1/4)
1154 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AspHI AvaI AvaII BalI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BsiEI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BspHI
BspMI BsrFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RcaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I