Restriction map for
pFN21AE6078
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# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MroI MspA1I MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI TfiI Tsp509I
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 225 GTCTAC GT^[AC][GT]AC
AciI 498 GCGG CCGC(-3/-1)
AfaI 950 GTAC GT^AC
AflIII 816 ACATGT A^C[GA][CT]GT
AgeI 920 ACCGGT A^CCGGT
AlwI 666 GATCC GGATC(4/5)
BanII 963 GGGCTC G[GA]GC[CT]^C
BbsI 888 GAAGAC GAAGAC(2/6)
BbvCI 717 CCTCAGC CCTCAGC(-5/-2)
BbvI 501 GCTGC GCAGC(8/12)
BcgI 457 CGAAGCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 250 CTAG C^TAG
BpuAI 888 GAAGAC GAAGAC(2/6)
BsaAI 902 TACGTG [CT]AC^GT[GA]
BsaWI 920 ACCGGT [AT]^CCGG[AT]
BspLU11I 816 ACATGT A^CATGT
BsrDI 279 CATTGC GCAATG(2/0)
BsrFI 920 ACCGGT [GA]^CCGG[CT]
BsrI 478 CCAGT ACTGG(1/-1)
Cfr10I 920 ACCGGT [GA]^CCGG[CT]
DsaI 356 CCATGG C^C[GA][CT]GG
HapII 921 CCGG C^CGG
HincII 586 GTTAAC GT[CT]^[GA]AC
HindII 586 GTTAAC GT[CT]^[GA]AC
HindIII 459 AAGCTT A^AGCTT
HpaI 586 GTTAAC GTT^AAC
HpaII 921 CCGG C^CGG
MaeI 250 CTAG C^TAG
MseI 587 TTAA T^TAA
MspI 921 CCGG C^CGG
NcoI 356 CCATGG C^CATGG
NdeI 319 CATATG CA^TATG
NlaIV 730 GGCTCC GG[ACGT]^[ACGT]CC
NspI 816 ACATGT [GA]CATG^[CT]
PflMI 254 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 920 ACCGGT A^CCGGT
RsaI 950 GTAC GT^AC
SspI 805 AATATT AAT^ATT
Tru9I 587 TTAA T^TAA
TseI 501 GCTGC G^C[AT]GC
Van91I 254 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 836 CCAATCTTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AluI 342 AGCT AG^CT
460 AGCT
BalI 377 TGGCCA TGG^CCA
833 TGGCCA
BstXI 614 CCATTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
789 CCATCATTTTGG
Cac8I 201 GCTGGC GC[ACGT]^[ACGT]GC
831 GCTGGC
Cfr13I 246 GGCCC G^G[ACGT]CC
714 GGCCC
DpnII 367 GATC ^GATC
666 GATC
DraII 245 AGGCCCT [GA]G^G[ACGT]CC[CT]
713 AGGCCCT
EcoO109I 245 AGGCCCT [GA]G^G[ACGT]CC[CT]
713 AGGCCCT
Fnu4HI 498 GCGGC GC^[ACGT]GC
501 GCTGC
HinfI 3 GAGTC G^A[ACGT]TC
337 GAGTC
HphI 299 TCACC GGTGA(8/7)
452 TCACC
ItaI 498 GCGGC GC^[ACGT]GC
501 GCTGC
MaeII 162 ACGT A^CGT
903 ACGT
MboI 367 GATC ^GATC
666 GATC
MluNI 377 TGGCCA TGG^CCA
833 TGGCCA
MscI 377 TGGCCA TGG^CCA
833 TGGCCA
NdeII 367 GATC ^GATC
666 GATC
PleI 3 GAGTC GAGTC(4/5)
337 GAGTC
PstI 32 CTGCAG CTGCA^G
116 CTGCAG
Sau3AI 367 GATC ^GATC
666 GATC
Sau96I 246 GGCCC G^G[ACGT]CC
714 GGCCC
SfcI 32 CTGCAG C^T[GA][CT]AG
116 CTGCAG
Tsp45I 5 GTCAC ^GT[GC]AC
298 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI DdeI EaeI
EcoRII EcoT14I FokI HaeIII MaeIII
MboII MnlI MslI MvaI MwoI
NlaIII ScrFI SfaNI StyI TspRI