Restriction map for
pFN21AB7131
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BbeI BbvCI
BcgI BciVI BclI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI Sau96I SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AflII 670 CTTAAG C^TTAAG
AflIII 691 ACACGT A^C[GA][CT]GT
BamHI 6 GGATCC G^GATCC
BanI 474 GGCACC G^G[CT][GA]CC
BbrPI 692 CACGTG CAC^GTG
BcnI 582 CCGGG CC^[GC]GG
BfaI 124 CTAG C^TAG
BfrI 670 CTTAAG C^TTAAG
BsaAI 692 CACGTG [CT]AC^GT[GA]
BsgI 464 CTGCAC GTGCAG(16/14)
BsmAI 727 GTCTC GTCTC(1/5)
BsmBI 726 CGTCTC CGTCTC(1/5)
BspHI 557 TCATGA T^CATGA
BsrBI 513 CCGCTC CCGCTC(-3/-3)
BsrDI 17 GCAATG GCAATG(2/0)
BssSI 422 CTCGTG CACGAG(-5/-1)
BstAPI 162 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 6 GGATCC [GA]^GATC[CT]
DpnII 7 GATC ^GATC
EarI 67 GAAGAG CTCTTC(1/4)
EcoRV 577 GATATC GAT^ATC
FauI 456 GCGGG CCCGC(4/6)
HapII 582 CCGG C^CGG
HgaI 725 GCGTC GACGC(5/10)
HpaII 582 CCGG C^CGG
Ksp632I 67 GAAGAG CTCTTC(1/4)
MaeI 124 CTAG C^TAG
MboI 7 GATC ^GATC
MfeI 869 CAATTG C^AATTG
MflI 6 GGATCC [GA]^GATC[CT]
MseI 671 TTAA T^TAA
MspI 582 CCGG C^CGG
MunI 869 CAATTG C^AATTG
NciI 582 CCGGG CC^[GC]GG
NdeII 7 GATC ^GATC
NspI 197 GCATGC [GA]CATG^[CT]
PflMI 254 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 692 CACGTG CAC^GTG
PmlI 692 CACGTG CAC^GTG
PvuII 499 CAGCTG CAG^CTG
RcaI 557 TCATGA T^CATGA
Sau3AI 7 GATC ^GATC
ScaI 408 AGTACT AGT^ACT
SfcI 301 CTACAG C^T[GA][CT]AG
SmlI 670 CTTAAG C^T[CT][GA]AG
SphI 197 GCATGC GCATG^C
StuI 51 AGGCCT AGG^CCT
Tru9I 671 TTAA T^TAA
Van91I 254 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 6 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 409 GTAC GT^AC
760 GTAC
AluI 500 AGCT AG^CT
563 AGCT
AlwI 7 GATCC GGATC(4/5)
6 GGATC
BanII 175 GAGCCC G[GA]GC[CT]^C
946 GAGCCC
BbsI 885 GAAGAC GAAGAC(2/6)
487 GTCTTC
BpuAI 885 GAAGAC GAAGAC(2/6)
487 GTCTTC
BsrI 26 CCAGT ACTGG(1/-1)
282 CCAGT
BstXI 242 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
623 CCATTGGCCTGG
CfoI 396 GCGC GCG^C
906 GCGC
DdeI 74 CTCAG C^T[ACGT]AG
158 CTGAG
DsaI 356 CCATGG C^C[GA][CT]GG
779 CCATGG
HhaI 396 GCGC GCG^C
906 GCGC
HinP1I 396 GCGC G^CGC
906 GCGC
HphI 387 TCACC GGTGA(8/7)
428 TCACC
MaeII 415 ACGT A^CGT
693 ACGT
MslI 547 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
733 CATGTGTGTG
MspA1I 499 CAGCTG C[AC]G^C[GT]G
814 CCGCTG
NcoI 356 CCATGG C^CATGG
779 CCATGG
PleI 634 GACTC GAGTC(4/5)
808 GACTC
RsaI 409 GTAC GT^AC
760 GTAC
TaqI 900 TCGA T^CGA
915 TCGA
TthHB8I 900 TCGA T^CGA
915 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AspHI BalI BbvI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MaeIII MboII MluNI
MnlI MscI MvaI MwoI NlaIII
NlaIV ScrFI SfaNI StyI TfiI
TseI Tsp45I Tsp509I TspRI XcmI