Restriction map for
pFN21AE5382
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MspA1I MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 243 GTCGAC GT^[AC][GT]AC
AccIII 674 TCCGGA T^CCGGA
AcsI 31 GAATTC [GA]^AATT[CT]
AlwNI 70 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 31 GAATTC [GA]^AATT[CT]
BanII 46 GGGCTC G[GA]GC[CT]^C
BbsI 264 GAAGAC GAAGAC(2/6)
BcnI 60 CCCGG CC^[GC]GG
BfaI 118 CTAG C^TAG
BmyI 46 GGGCTC G[AGT]GC[ACT]^C
BpuAI 264 GAAGAC GAAGAC(2/6)
BsaJI 126 CCTGGG C^C[ACGT][ACGT]GG
BseAI 674 TCCGGA T^CCGGA
BsmFI 754 GGGAC GGGAC(10/14)
BsmI 706 GCATTC GAATGC(1/-1)
Bsp1286I 46 GGGCTC G[AGT]GC[ACT]^C
BspEI 674 TCCGGA T^CCGGA
BsrBI 544 CCGCTC CCGCTC(-3/-3)
BstXI 632 CCATCGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 505 AGATCC [GA]^GATC[CT]
CfoI 387 GCGC GCG^C
EarI 466 CTCTTC CTCTTC(1/4)
EcoRI 31 GAATTC G^AATTC
HhaI 387 GCGC GCG^C
HinP1I 387 GCGC G^CGC
HincII 243 GTCGAC GT[CT]^[GA]AC
HindII 243 GTCGAC GT[CT]^[GA]AC
HindIII 553 AAGCTT A^AGCTT
Ksp632I 466 CTCTTC CTCTTC(1/4)
MaeI 118 CTAG C^TAG
MaeII 403 ACGT A^CGT
MflI 505 AGATCC [GA]^GATC[CT]
MroI 674 TCCGGA T^CCGGA
NciI 60 CCCGG CC^[GC]GG
NdeI 727 CATATG CA^TATG
NlaIV 721 GGTTCC GG[ACGT]^[ACGT]CC
PflMI 726 CCATATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 243 GTCGAC G^TCGAC
SfaNI 419 GCATC GCATC(5/9)
SphI 585 GCATGC GCATG^C
StuI 893 AGGCCT AGG^CCT
TaqI 244 TCGA T^CGA
TfiI 805 GAATC G^A[AT]TC
Tsp509I 32 AATT ^AATT
TthHB8I 244 TCGA T^CGA
Van91I 726 CCATATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 505 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 177 GTAC GT^AC
847 GTAC
AlwI 506 GATCC GGATC(4/5)
677 GGATC
BalI 368 TGGCCA TGG^CCA
428 TGGCCA
BbvI 343 GCAGC GCAGC(8/12)
664 GCTGC
BpmI 67 CTCCAG CTGGAG(16/14)
618 CTCCAG
BsaWI 674 TCCGGA [AT]^CCGG[AT]
843 TCCGGT
BsmAI 147 GAGAC GTCTC(1/5)
72 GTCTC
BsrDI 309 CATTGC GCAATG(2/0)
346 GCAATG
BstF5I 477 CATCC GGATG(2/0)
669 CATCC
EaeI 368 TGGCCA [CT]^GGCC[GA]
428 TGGCCA
FauI 234 CCCGC CCCGC(4/6)
540 CCCGC
FokI 477 CATCC GGATG(9/13)
669 CATCC
MaeIII 109 GTCAC ^GT[ACGT]AC
739 GTCAC
MluNI 368 TGGCCA TGG^CCA
428 TGGCCA
MscI 368 TGGCCA TGG^CCA
428 TGGCCA
RsaI 177 GTAC GT^AC
847 GTAC
TseI 343 GCAGC G^C[AT]GC
664 GCTGC
Tsp45I 109 GTCAC ^GT[GC]AC
739 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI BsiYI BslI BsrI
BssKI BstNI Cac8I DdeI DpnII
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NspI PleI
Sau3AI ScrFI SfcI Tru9I TspRI