Restriction map for
pFN21AE3446
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BbeI BbrPI BbsI BbvCI BciVI
BfaI BfrI BglI BlnI BpuAI
BsaAI BsaHI BsaI BseAI BseRI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeII
MluI MroI MvnI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 367 GTAGAC GT^[AC][GT]AC
ApaI 1074 GGGCCC GGGCC^C
AspI 1106 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 752 GGATCC G^GATCC
BanI 8 GGCACC G^G[CT][GA]CC
BcgI 977 GCACGAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 381 CCGGG CC^[GC]GG
BglII 413 AGATCT A^GATCT
BlpI 620 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 620 GCTCAGC GC^T[ACGT]AGC
BsaBI 753 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 772 CTGCAC GTGCAG(16/14)
BsmAI 651 GAGAC GTCTC(1/5)
BsrBI 807 CCGCTC CCGCTC(-3/-3)
BsrI 275 ACTGG ACTGG(1/-1)
BssSI 978 CACGAG CACGAG(-5/-1)
BstAPI 9 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 620 GCTCAGC GC^T[ACGT]AGC
CfoI 1019 GCGC GCG^C
DraII 998 GGGACCC [GA]G^G[ACGT]CC[CT]
EarI 310 CTCTTC CTCTTC(1/4)
EcoO109I 998 GGGACCC [GA]G^G[ACGT]CC[CT]
HaeII 1018 GGCGCT [GA]GCGC^[CT]
HgaI 983 GCGTC GACGC(5/10)
HhaI 1019 GCGC GCG^C
HinP1I 1019 GCGC G^CGC
HinfI 3 GAATC G^A[ACGT]TC
Ksp632I 310 CTCTTC CTCTTC(1/4)
MaeIII 1112 GTCAC ^GT[ACGT]AC
MamI 753 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 420 CAATTG C^AATTG
MseI 1033 TTAA T^TAA
MspA1I 930 CCGCTG C[AC]G^C[GT]G
MunI 420 CAATTG C^AATTG
NaeI 299 GCCGGC GCC^GGC
NciI 381 CCGGG CC^[GC]GG
NcoI 1054 CCATGG C^CATGG
NgoMIV 299 GCCGGC G^CCGGC
PflFI 1106 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 998 GGGACCC [GA]G^G[AT]CC[CT]
PstI 16 CTGCAG CTGCA^G
SacI 988 GAGCTC GAGCT^C
SapI 309 GCTCTTC GCTCTTC(1/4)
SfcI 16 CTGCAG C^T[GA][CT]AG
SgrAI 92 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 166 CTCAAG C^T[CT][GA]AG
TfiI 3 GAATC G^A[AT]TC
Tru9I 1033 TTAA T^TAA
Tsp45I 1112 GTCAC ^GT[GC]AC
TspRI 462 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1106 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 804 GTAC GT^AC
879 GTAC
AlwNI 209 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
540 CAGCATCTG
AspHI 307 GTGCTC G[AT]GC[AT]^C
988 GAGCTC
BalI 707 TGGCCA TGG^CCA
1037 TGGCCA
BclI 227 TGATCA T^GATCA
923 TGATCA
BsaWI 557 TCCGGT [AT]^CCGG[AT]
1045 TCCGGT
BsiHKAI 307 GTGCTC G[AT]GC[AT]^C
988 GAGCTC
BsrDI 75 CATTGC GCAATG(2/0)
131 GCAATG
BstYI 413 AGATCT [GA]^GATC[CT]
752 GGATCC
DdeI 621 CTCAG C^T[ACGT]AG
838 CTCAG
DsaI 872 CCGTGG C^C[GA][CT]GG
1054 CCATGG
EaeI 707 TGGCCA [CT]^GGCC[GA]
1037 TGGCCA
EcoT14I 80 CCAAGG C^C[AT][AT]GG
1054 CCATGG
FbaI 227 TGATCA T^GATCA
923 TGATCA
MflI 413 AGATCT [GA]^GATC[CT]
752 GGATCC
MluNI 707 TGGCCA TGG^CCA
1037 TGGCCA
MscI 707 TGGCCA TGG^CCA
1037 TGGCCA
NspI 666 GCATGC [GA]CATG^[CT]
860 GCATGC
RsaI 804 GTAC GT^AC
879 GTAC
SphI 666 GCATGC GCATG^C
860 GCATGC
StyI 80 CCAAGG C^C[AT][AT]GG
1054 CCATGG
TaqI 986 TCGA T^CGA
1157 TCGA
TthHB8I 986 TCGA T^CGA
1157 TCGA
XhoII 413 AGATCT [GA]^GATC[CT]
752 GGATCC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BmyI BpmI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrFI BssKI
BstF5I BstNI Cac8I Cfr10I Cfr13I
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MboI
MboII MnlI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TseI Tsp509I