Restriction map for
pFN21AE9521
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BcgI BciVI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BseRI BsiWI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HincII HindII HindIII HinfI
HpaI KpnI MaeI MamI MfeI
MluI MroI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PleI PmeI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 406 GGCGCC G[GA]^CG[CT]C
Alw44I 311 GTGCAC G^TGCAC
ApaI 762 GGGCCC GGGCC^C
ApaLI 311 GTGCAC G^TGCAC
AspI 349 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 13 GGATCC G^GATCC
BbeI 406 GGCGCC GGCGC^C
BbvCI 135 CCTCAGC CCTCAGC(-5/-2)
BclI 365 TGATCA T^GATCA
BsaHI 406 GGCGCC G[GA]^CG[CT]C
BsaWI 197 ACCGGA [AT]^CCGG[AT]
BsiEI 284 CGGCCG CG[GA][CT]^CG
BsmAI 280 GAGAC GTCTC(1/5)
BsmBI 280 GAGACG CGTCTC(1/5)
BsrBI 261 GAGCGG CCGCTC(-3/-3)
BsrDI 866 GCAATG GCAATG(2/0)
BsrFI 307 GCCGGT [GA]^CCGG[CT]
BssSI 574 CTCGTG CACGAG(-5/-1)
BstYI 13 GGATCC [GA]^GATC[CT]
Cfr10I 307 GCCGGT [GA]^CCGG[CT]
DdeI 136 CTCAG C^T[ACGT]AG
EagI 284 CGGCCG C^GGCCG
EclXI 284 CGGCCG C^GGCCG
Eco52I 284 CGGCCG C^GGCCG
EcoNI 297 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 365 TGATCA T^GATCA
HgaI 921 GACGC GACGC(5/10)
Hin1I 406 GGCGCC G[GA]^CG[CT]C
KasI 406 GGCGCC G^GCGCC
KspI 475 CCGCGG CCGC^GG
MflI 13 GGATCC [GA]^GATC[CT]
MseI 710 TTAA T^TAA
NarI 406 GGCGCC GG^CGCC
PflFI 349 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 624 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 282 GACGGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 910 GAGCTC GAGCT^C
SacII 475 CCGCGG CCGC^GG
SfoI 406 GGCGCC GGC^GCC
SmlI 925 CTCAAG C^T[CT][GA]AG
TaqI 878 TCGA T^CGA
Tru9I 710 TTAA T^TAA
Tth111I 349 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 878 TCGA T^CGA
Van91I 624 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 13 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 36 enzymes) #
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AfaI 219 GTAC GT^AC
618 GTAC
AlwI 14 GATCC GGATC(4/5)
13 GGATC
AlwNI 340 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
390 CAGCCTCTG
BalI 179 TGGCCA TGG^CCA
509 TGGCCA
BanI 101 GGTGCC G^G[CT][GA]CC
406 GGCGCC
BbrPI 553 CACGTG CAC^GTG
813 CACGTG
BglI 490 GCCGTTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
707 GCCTTAAAGGC
BsaAI 553 CACGTG [CT]AC^GT[GA]
813 CACGTG
BsgI 205 CTGCAC GTGCAG(16/14)
7 GTGCAG
BsrI 315 ACTGG ACTGG(1/-1)
512 CCAGT
DpnII 14 GATC ^GATC
366 GATC
DraII 761 GGGGCCC [GA]G^G[ACGT]CC[CT]
762 GGGCCCC
DsaI 416 CCGTGG C^C[GA][CT]GG
475 CCGCGG
EarI 130 CTCTTC CTCTTC(1/4)
532 CTCTTC
EcoO109I 761 GGGGCCC [GA]G^G[ACGT]CC[CT]
762 GGGCCCC
FauI 263 GCGGG CCCGC(4/6)
477 GCGGG
Ksp632I 130 CTCTTC CTCTTC(1/4)
532 CTCTTC
MaeIII 466 GTGAC ^GT[ACGT]AC
721 GTCAC
MboI 14 GATC ^GATC
366 GATC
MluNI 179 TGGCCA TGG^CCA
509 TGGCCA
MscI 179 TGGCCA TGG^CCA
509 TGGCCA
NdeII 14 GATC ^GATC
366 GATC
NspI 626 ACATGC [GA]CATG^[CT]
940 ACATGC
PmaCI 553 CACGTG CAC^GTG
813 CACGTG
PmlI 553 CACGTG CAC^GTG
813 CACGTG
PstI 337 CTGCAG CTGCA^G
372 CTGCAG
PvuII 67 CAGCTG CAG^CTG
343 CAGCTG
RsaI 219 GTAC GT^AC
618 GTAC
SapI 129 GCTCTTC GCTCTTC(1/4)
531 GCTCTTC
Sau3AI 14 GATC ^GATC
366 GATC
SfcI 337 CTGCAG C^T[GA][CT]AG
372 CTGCAG
SmaI 323 CCCGGG CCC^GGG
635 CCCGGG
Tsp45I 466 GTGAC ^GT[GC]AC
721 GTCAC
XcmI 59 CCATCCCCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
693 CCACCAGGGCTTTGG
XmaCI 323 CCCGGG C^CCGGG
635 CCCGGG
XmaI 323 CCCGGG C^CCGGG
635 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BspMI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr13I EaeI EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV Sau96I ScrFI SfaNI
TseI TspRI