Restriction map for
pFN21AE5366
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI Ksp632I KspI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tsp45I TspRI Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AciI 412 GCGG CCGC(-3/-1)
AcsI 322 AAATTC [GA]^AATT[CT]
AlwNI 343 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 322 AAATTC [GA]^AATT[CT]
Asp700I 435 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 40 GTGCTC G[AT]GC[AT]^C
BfaI 316 CTAG C^TAG
BglII 260 AGATCT A^GATCT
BlpI 42 GCTCAGC GC^T[ACGT]AGC
BmyI 40 GTGCTC G[AGT]GC[ACT]^C
BpmI 224 CTCCAG CTGGAG(16/14)
Bpu1102I 42 GCTCAGC GC^T[ACGT]AGC
BsaI 85 GAGACC GGTCTC(1/5)
BsgI 327 CTGCAC GTGCAG(16/14)
BsiHKAI 40 GTGCTC G[AT]GC[AT]^C
BsiYI 90 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 90 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 85 GAGAC GTCTC(1/5)
BsmI 408 GAATGC GAATGC(1/-1)
Bsp1286I 40 GTGCTC G[AGT]GC[ACT]^C
BspHI 515 TCATGA T^CATGA
BsrI 210 CCAGT ACTGG(1/-1)
BstF5I 303 GGATG GGATG(2/0)
CelII 42 GCTCAGC GC^T[ACGT]AGC
FokI 303 GGATG GGATG(9/13)
HaeIII 6 GGCC GG^CC
MaeI 316 CTAG C^TAG
MseI 556 TTAA T^TAA
NlaIV 222 GGCTCC GG[ACGT]^[ACGT]CC
PflMI 90 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 415 GACTC GAGTC(4/5)
RcaI 515 TCATGA T^CATGA
Tru9I 556 TTAA T^TAA
Tsp509I 323 AATT ^AATT
Van91I 90 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 435 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwI 252 GATCC GGATC(4/5)
521 GATCC
BsaJI 188 CCCAGG C^C[ACGT][ACGT]GG
491 CCTGGG
BseRI 193 GAGGAG GAGGAG(10/8)
379 GAGGAG
BstYI 260 AGATCT [GA]^GATC[CT]
520 AGATCC
DdeI 43 CTCAG C^T[ACGT]AG
83 CTGAG
DraII 109 AGGACCT [GA]G^G[ACGT]CC[CT]
308 AGGACCT
EcoO109I 109 AGGACCT [GA]G^G[ACGT]CC[CT]
308 AGGACCT
MflI 260 AGATCT [GA]^GATC[CT]
520 AGATCC
MspA1I 45 CAGCTG C[AC]G^C[GT]G
346 CAGCTG
PpuMI 109 AGGACCT [GA]G^G[AT]CC[CT]
308 AGGACCT
PvuII 45 CAGCTG CAG^CTG
346 CAGCTG
SfaNI 117 GATGC GCATC(5/9)
372 GATGC
XhoII 260 AGATCT [GA]^GATC[CT]
520 AGATCC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI AvaII BbvI BssKI BstNI
Cfr13I DpnII EcoRII Fnu4HI HinfI
ItaI MboI MboII MnlI MvaI
MwoI NdeII NlaIII Sau3AI Sau96I
ScrFI TfiI TseI