Restriction map for
pFN21AE5333
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BseAI BsiHKAI BsiWI
BsmFI BsmI BspDI BspEI BspLU11I
BsrBI BsrDI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DraIII DrdI Eam1105I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
SwaI TaqI TfiI Tru9I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcyI 710 GGCGCC G[GA]^CG[CT]C
AflIII 746 ACGTGT A^C[GA][CT]GT
AluI 496 AGCT AG^CT
Aor51HI 605 AGCGCT AGC^GCT
Asp700I 226 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 472 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 290 TGCGCA TGC^GCA
BanI 710 GGCGCC G^G[CT][GA]CC
BanII 723 GAGCCC G[GA]GC[CT]^C
BbeI 710 GGCGCC GGCGC^C
BbsI 282 GAAGAC GAAGAC(2/6)
BlpI 33 GCTGAGC GC^T[ACGT]AGC
BmyI 723 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 33 GCTGAGC GC^T[ACGT]AGC
BpuAI 282 GAAGAC GAAGAC(2/6)
BsaHI 710 GGCGCC G[GA]^CG[CT]C
BsaWI 240 ACCGGA [AT]^CCGG[AT]
BsiEI 717 CGGCCG CG[GA][CT]^CG
BsmBI 658 CGTCTC CGTCTC(1/5)
Bsp1286I 723 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 101 TGTACA T^GTACA
BspHI 479 TCATGA T^CATGA
BsrGI 101 TGTACA T^GTACA
BsrI 95 ACTGG ACTGG(1/-1)
BssSI 328 CTCGTG CACGAG(-5/-1)
BstNI 66 CCTGG CC^[AT]GG
CelII 33 GCTGAGC GC^T[ACGT]AGC
DpnII 246 GATC ^GATC
EagI 717 CGGCCG C^GGCCG
EarI 322 CTCTTC CTCTTC(1/4)
EclXI 717 CGGCCG C^GGCCG
Eco47III 605 AGCGCT AGC^GCT
Eco52I 717 CGGCCG C^GGCCG
EcoRII 66 CCTGG ^CC[AT]GG
FspI 290 TGCGCA TGC^GCA
Hin1I 710 GGCGCC G[GA]^CG[CT]C
KasI 710 GGCGCC G^GCGCC
Ksp632I 322 CTCTTC CTCTTC(1/4)
KspI 714 CCGCGG CCGC^GG
MaeII 746 ACGT A^CGT
MboI 246 GATC ^GATC
MslI 753 CATCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 714 CCGCGG C[AC]G^C[GT]G
MvaI 66 CCTGG CC^[AT]GG
NarI 710 GGCGCC GG^CGCC
NcoI 311 CCATGG C^CATGG
NdeII 246 GATC ^GATC
PflFI 472 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 479 TCATGA T^CATGA
SacII 714 CCGCGG CCGC^GG
Sau3AI 246 GATC ^GATC
SfoI 710 GGCGCC GGC^GCC
SspBI 101 TGTACA T^GTACA
Tsp509I 250 AATT ^AATT
Tth111I 472 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 226 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 663 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
688 CAGTTCCTG
AvaI 681 CTCGGG C^[CT]CG[GA]G
700 CCCGAG
AvaII 380 GGACC G^G[AT]CC
795 GGACC
BcnI 639 CCCGG CC^[GC]GG
792 CCCGG
BpmI 459 CTCCAG CTGGAG(16/14)
738 CTCCAG
BseRI 616 GAGGAG GAGGAG(10/8)
619 GAGGAG
BsgI 160 CTGCAC GTGCAG(16/14)
802 CTGCAC
BsmAI 397 GTCTC GTCTC(1/5)
659 GTCTC
BsoBI 681 CTCGGG C^[CT]CG[GA]G
700 CCCGAG
BspMI 800 ACCTGC ACCTGC(4/8)
193 GCAGGT
BsrFI 166 GCCGGC [GA]^CCGG[CT]
574 GCCGGC
BstF5I 366 CATCC GGATG(2/0)
753 CATCC
Cfr10I 166 GCCGGC [GA]^CCGG[CT]
574 GCCGGC
DsaI 311 CCATGG C^C[GA][CT]GG
714 CCGCGG
EcoT14I 311 CCATGG C^C[AT][AT]GG
431 CCTTGG
FokI 366 CATCC GGATG(9/13)
753 CATCC
HaeII 605 AGCGCT [GA]GCGC^[CT]
710 GGCGCC
HgaI 762 GACGC GACGC(5/10)
657 GCGTC
HinfI 3 GAGTC G^A[ACGT]TC
179 GAGTC
HphI 255 GGTGA GGTGA(8/7)
599 TCACC
MaeIII 5 GTCAC ^GT[ACGT]AC
256 GTGAC
NaeI 166 GCCGGC GCC^GGC
574 GCCGGC
NciI 639 CCCGG CC^[GC]GG
792 CCCGG
NgoMIV 166 GCCGGC G^CCGGC
574 GCCGGC
PleI 3 GAGTC GAGTC(4/5)
179 GAGTC
PstI 9 CTGCAG CTGCA^G
694 CTGCAG
SfcI 9 CTGCAG C^T[GA][CT]AG
694 CTGCAG
SspI 227 AATATT AAT^ATT
274 AATATT
StyI 311 CCATGG C^C[AT][AT]GG
431 CCTTGG
Tsp45I 5 GTCAC ^GT[GC]AC
256 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AccII AciI AfaI BbvI BsaJI
BsiYI BslI BssKI BstUI Cac8I
CfoI Cfr13I DdeI EaeI FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MboII MnlI MspI
MvnI MwoI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI TseI TspRI