Restriction map for
pFN21AE5296
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BmyI BpmI BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BsrI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HapII Hin1I HindIII HpaI
HpaII HphI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tsp509I TspRI Tth111I
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 162 GTAC GT^AC
AflIII 386 ACATGT A^C[GA][CT]GT
AlwNI 214 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 100 CCTGAGG CC^T[ACGT]AGG
Aor51HI 6 AGCGCT AGC^GCT
Asp700I 197 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfaI 119 CTAG C^TAG
BglII 363 AGATCT A^GATCT
BseRI 103 GAGGAG GAGGAG(10/8)
BsgI 42 CTGCAC GTGCAG(16/14)
BspLU11I 386 ACATGT A^CATGT
BsrDI 378 CATTGC GCAATG(2/0)
BssSI 110 CTCGTG CACGAG(-5/-1)
BstYI 363 AGATCT [GA]^GATC[CT]
Bsu36I 100 CCTGAGG CC^T[ACGT]AGG
Cfr13I 455 GGCCC G^G[ACGT]CC
DpnII 364 GATC ^GATC
Eco47III 6 AGCGCT AGC^GCT
Eco81I 100 CCTGAGG CC^T[ACGT]AGG
HgaI 122 GCGTC GACGC(5/10)
HincII 313 GTCAAC GT[CT]^[GA]AC
HindII 313 GTCAAC GT[CT]^[GA]AC
MaeI 119 CTAG C^TAG
MaeIII 25 GTCAC ^GT[ACGT]AC
MboI 364 GATC ^GATC
MflI 363 AGATCT [GA]^GATC[CT]
MslI 153 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 364 GATC ^GATC
PaeR7I 69 CTCGAG C^TCGAG
PleI 106 GAGTC GAGTC(4/5)
PstI 206 CTGCAG CTGCA^G
RsaI 162 GTAC GT^AC
Sau3AI 364 GATC ^GATC
Sau96I 455 GGCCC G^G[ACGT]CC
SfcI 206 CTGCAG C^T[GA][CT]AG
TaqI 70 TCGA T^CGA
TfiI 197 GAATC G^A[AT]TC
Tsp45I 25 GTCAC ^GT[GC]AC
TthHB8I 70 TCGA T^CGA
XhoI 69 CTCGAG C^TCGAG
XhoII 363 AGATCT [GA]^GATC[CT]
XmnI 197 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 155 CCGC CCGC(-3/-1)
251 GCGG
AvaI 69 CTCGAG C^[CT]CG[GA]G
263 CCCGAG
BlpI 211 GCTCAGC GC^T[ACGT]AGC
369 GCTGAGC
Bpu1102I 211 GCTCAGC GC^T[ACGT]AGC
369 GCTGAGC
BsiYI 244 CCAACAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
457 CCCATCACAGG
BslI 244 CCAACAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
457 CCCATCACAGG
BsoBI 69 CTCGAG C^[CT]CG[GA]G
263 CCCGAG
BssKI 144 CCTGG ^CC[ACGT]GG
438 CCTGG
BstNI 144 CCTGG CC^[AT]GG
438 CCTGG
CelII 211 GCTCAGC GC^T[ACGT]AGC
369 GCTGAGC
CfoI 7 GCGC GCG^C
141 GCGC
EcoRII 144 CCTGG ^CC[AT]GG
438 CCTGG
HaeII 6 AGCGCT [GA]GCGC^[CT]
140 AGCGCC
HaeIII 39 GGCC GG^CC
455 GGCC
HhaI 7 GCGC GCG^C
141 GCGC
HinP1I 7 GCGC G^CGC
141 GCGC
HinfI 106 GAGTC G^A[ACGT]TC
197 GAATC
MboII 361 GAAGA GAAGA(8/7)
56 TCTTC
MvaI 144 CCTGG CC^[AT]GG
438 CCTGG
NspI 386 ACATGT [GA]CATG^[CT]
407 ACATGC
ScrFI 144 CCTGG CC^[ACGT]GG
438 CCTGG
SmlI 69 CTCGAG C^T[CT][GA]AG
184 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BbvI BsmAI BstF5I Cac8I
DdeI Fnu4HI FokI ItaI MnlI
MwoI NlaIII SfaNI TseI