Restriction map for
pFN21AE5180
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DraI DraIII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 196 GTAGAC GT^[AC][GT]AC
AlwNI 256 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 304 AGCGCT AGC^GCT
ApaI 12 GGGCCC GGGCC^C
AvaII 445 GGACC G^G[AT]CC
BanII 12 GGGCCC G[GA]GC[CT]^C
BbrPI 229 CACGTG CAC^GTG
BglI 366 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 12 GGGCCC G[AGT]GC[ACT]^C
BsaAI 229 CACGTG [CT]AC^GT[GA]
BsaI 88 GGTCTC GGTCTC(1/5)
BsaWI 442 ACCGGA [AT]^CCGG[AT]
BseRI 273 GAGGAG GAGGAG(10/8)
BsgI 28 GTGCAG GTGCAG(16/14)
BsmAI 89 GTCTC GTCTC(1/5)
BsmFI 252 GTCCC GGGAC(10/14)
Bsp1286I 12 GGGCCC G[AGT]GC[ACT]^C
BspMI 30 GCAGGT ACCTGC(4/8)
BsrDI 83 GCAATG GCAATG(2/0)
BstAPI 21 GCAGTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 416 GATC ^GATC
DraII 11 AGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 199 GACATCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 304 AGCGCT AGC^GCT
EcoO109I 11 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 8 CCAAGG C^C[AT][AT]GG
FauI 15 CCCGC CCCGC(4/6)
HaeII 304 AGCGCT [GA]GCGC^[CT]
HapII 443 CCGG C^CGG
HgaI 41 GACGC GACGC(5/10)
HinfI 250 GAGTC G^A[ACGT]TC
HpaII 443 CCGG C^CGG
MaeII 230 ACGT A^CGT
MaeIII 227 GTCAC ^GT[ACGT]AC
MboI 416 GATC ^GATC
MseI 320 TTAA T^TAA
MslI 93 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 443 CCGG C^CGG
NdeII 416 GATC ^GATC
NlaIII 269 CATG CATG^
PflMI 255 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 250 GAGTC GAGTC(4/5)
PmaCI 229 CACGTG CAC^GTG
PmlI 229 CACGTG CAC^GTG
PstI 19 CTGCAG CTGCA^G
PvuII 59 CAGCTG CAG^CTG
Sau3AI 416 GATC ^GATC
StuI 294 AGGCCT AGG^CCT
StyI 8 CCAAGG C^C[AT][AT]GG
Tru9I 320 TTAA T^TAA
Tsp45I 227 GTCAC ^GT[GC]AC
Tsp509I 322 AATT ^AATT
TspRI 70 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 255 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 361 CCAAAGCCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccII 79 CGCG CG^CG
131 CGCG
AfaI 144 GTAC GT^AC
313 GTAC
BbsI 48 GAAGAC GAAGAC(2/6)
34 GTCTTC
BbvI 18 GCTGC GCAGC(8/12)
150 GCTGC
BpmI 115 CTGGAG CTGGAG(16/14)
154 CTGGAG
BpuAI 48 GAAGAC GAAGAC(2/6)
34 GTCTTC
BstUI 79 CGCG CG^CG
131 CGCG
BstXI 237 CCAGATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
361 CCAAAGCCCTGG
Cac8I 14 GCCCGC GC[ACGT]^[ACGT]GC
134 GCACGC
HphI 99 GGTGA GGTGA(8/7)
247 GGTGA
MboII 48 GAAGA GAAGA(8/7)
35 TCTTC
MspA1I 16 CCGCTG C[AC]G^C[GT]G
59 CAGCTG
MvnI 79 CGCG CG^CG
131 CGCG
RsaI 144 GTAC GT^AC
313 GTAC
SfcI 19 CTGCAG C^T[GA][CT]AG
139 CTACAG
TseI 18 GCTGC G^C[AT]GC
150 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI CfoI
Cfr13I EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I ItaI MnlI MvaI
MwoI NlaIV Sau96I ScrFI