##################################
# NO CUT ENZYMES (   56 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AgeI        ApoI        AscI        AseI        AsnI        
Asp718I     BbrPI       BciVI       BfrI        BseAI       
BspDI       BspEI       BssHII      Bst1107I    BstBI       
BstZ17I     ClaI        DraI        EcoRI       EcoRV       
HpaI        KpnI        MfeI        MluI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        SalI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        SspI        SwaI        TfiI        Tsp509I     
XbaI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

Alw44I       4449  GTGCAC               G^TGCAC
AocI          546  CCTGAGG              CC^T[ACGT]AGG
ApaLI        4449  GTGCAC               G^TGCAC
AvrII        2769  CCTAGG               C^CTAGG
BlnI         2769  CCTAGG               C^CTAGG
BlpI           49  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       49  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1093  TACGTG               [CT]AC^GT[GA]
BsaWI        5798  TCCGGT               [AT]^CCGG[AT]
BsiWI         979  CGTACG               C^GTACG
Bsp1407I     4157  TGTACA               T^GTACA
BspLU11I     4271  ACATGT               A^CATGT
BsrGI        4157  TGTACA               T^GTACA
Bsu36I        546  CCTGAGG              CC^T[ACGT]AGG
CelII          49  GCTGAGC              GC^T[ACGT]AGC
CpoI         6545  CGGACCG              CG^G[AT]CCG
Eco81I        546  CCTGAGG              CC^T[ACGT]AGG
EcoT22I      2341  ATGCAT               ATGCA^T
FseI         6168  GGCCGGCC             GGCCGG^CC
HindIII      4841  AAGCTT               A^AGCTT
MseI         5422  TTAA                 T^TAA
NsiI         2341  ATGCAT               ATGCA^T
RsrII        6545  CGGACCG              CG^G[AT]CCG
SbfI          342  CCTGCAGG             CCTGCA^GG
Sse8387I      342  CCTGCAGG             CCTGCA^GG
SspBI        4157  TGTACA               T^GTACA
Tru9I        5422  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccI          898  GTCTAC               GT^[AC][GT]AC
             3448  GTCTAC              
AflIII       4271  ACATGT               A^C[GA][CT]GT
             6041  ACACGT              
Aor51HI      1250  AGCGCT               AGC^GCT
             2891  AGCGCT              
Asp700I      2403  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3468  GAACAGGTTC          
BamHI         587  GGATCC               G^GATCC
             5748  GGATCC              
BbvCI        3779  CCTCAGC              CCTCAGC(-5/-2)
             6378  CCTCAGC             
BfaI         2770  CTAG                 C^TAG
             2869  CTAG                
BsmI         4811  GAATGC               GAATGC(1/-1)
             5710  GAATGC              
BstEII       4503  GGTCACC              G^GT[ACGT]ACC
             5993  GGTCACC             
BstPI        4503  GGTCACC              G^GT[ACGT]ACC
             5993  GGTCACC             
DraIII       2756  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3372  CACCCTGTG           
Eco47III     1250  AGCGCT               AGC^GCT
             2891  AGCGCT              
EcoO65I      4503  GGTCACC              G^GT[ACGT]ACC
             5993  GGTCACC             
MaeI         2770  CTAG                 C^TAG
             2869  CTAG                
XmnI         2403  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3468  GAACAGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  160 enzymes) #
#########################################

AatII       AccII       AciI        AcyI        AfaI        
AhdI        AluI        AlwI        AlwNI       ApaI        
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       BalI        BanI        BanII       BbeI        
BbsI        BbvI        BcgI        BclI        BcnI        
BglI        BglII       BmyI        BpmI        BpuAI       
BsaBI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspHI       BspMI       BsrBI       BsrDI       BsrFI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco52I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     KspI        MaeII       
MaeIII      MamI        MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PaeR7I      PflFI       
PflMI       PleI        PpuMI       PshAI       PstI        
PvuII       RcaI        RsaI        SacI        SacII       
SapI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SfoI        SmaI        SmlI        
SphI        StuI        StyI        TaqI        TseI        
Tsp45I      TspRI       Tth111I     TthHB8I     Van91I      
XcmI        XhoI        XhoII       XmaCI       XmaI