Restriction map for
pFN21AE4296
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# NO CUT ENZYMES ( 44 enzymes) #
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AclI AgeI Aor51HI AscI AviII
BbeI BsiWI BssHII Bst1107I BstBI
BstZ17I CpoI DrdI EagI EclXI
Eco47III Eco52I FseI FspI HpaI
KasI KspI MluI NarI NdeI
NotI NruI NspV PacI PinAI
PmeI Psp1406I PvuI RsrII SacII
SbfI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI
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# ONE CUT ENZYMES ( 25 enzymes) #
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AatII 2977 GACGTC GACGT^C
Acc65I 1747 GGTACC G^GTACC
AflII 6438 CTTAAG C^TTAAG
AseI 5125 ATTAAT AT^TAAT
AsnI 5125 ATTAAT AT^TAAT
Asp718I 1747 GGTACC G^GTACC
BciVI 3877 GGATAC GTATCC(6/5)
BfrI 6438 CTTAAG C^TTAAG
BglII 5769 AGATCT A^GATCT
EcoT22I 2890 ATGCAT ATGCA^T
KpnI 1747 GGTACC GGTAC^C
MfeI 3934 CAATTG C^AATTG
MunI 3934 CAATTG C^AATTG
NheI 6426 GCTAGC G^CTAGC
NsiI 2890 ATGCAT ATGCA^T
PaeR7I 496 CTCGAG C^TCGAG
PshAI 1179 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5125 ATTAAT AT^TAAT
SalI 6016 GTCGAC G^TCGAC
ScaI 5149 AGTACT AGT^ACT
SfiI 7497 GGCCGTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 6659 CCCGGG CCC^GGG
XhoI 496 CTCGAG C^TCGAG
XmaCI 6659 CCCGGG C^CCGGG
XmaI 6659 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccII 9180 CGCG CG^CG
9201 CGCG
AhdI 406 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8833 GACAGCGAGTC
AspEI 406 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8833 GACAGCGAGTC
AvrII 3873 CCTAGG C^CTAGG
6444 CCTAGG
BlnI 3873 CCTAGG C^CTAGG
6444 CCTAGG
BsiEI 9137 CGGTCG CG[GA][CT]^CG
9315 CGACCG
BspDI 2263 ATCGAT AT^CGAT
2545 ATCGAT
BstEII 7253 GGTCACC G^GT[ACGT]ACC
9408 GGTCACC
BstPI 7253 GGTCACC G^GT[ACGT]ACC
9408 GGTCACC
BstUI 9180 CGCG CG^CG
9201 CGCG
ClaI 2263 ATCGAT AT^CGAT
2545 ATCGAT
DraI 2694 TTTAAA TTT^AAA
4290 TTTAAA
Eam1105I 406 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8833 GACAGCGAGTC
EcoO65I 7253 GGTCACC G^GT[ACGT]ACC
9408 GGTCACC
EcoRI 4541 GAATTC G^AATTC
5018 GAATTC
EcoRV 1576 GATATC GAT^ATC
5010 GATATC
MvnI 9180 CGCG CG^CG
9201 CGCG
NaeI 7146 GCCGGC GCC^GGC
7549 GCCGGC
NgoMIV 7146 GCCGGC G^CCGGC
7549 GCCGGC
SspI 848 AATATT AAT^ATT
7693 AATATT
XbaI 3622 TCTAGA T^CTAGA
8943 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 168 enzymes) #
#########################################
AccI AccIII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbrPI
BbsI BbvCI BbvI BcgI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmnI