Restriction map for
pFN21AE6758
##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI BbrPI
BclI BfrI BglI BglII BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FauI FbaI FseI HindIII HpaI
KpnI KspI MaeII MamI MfeI
MluI MroI MunI MvnI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcyI 1374 GGCGCC G[GA]^CG[CT]C
AflIII 301 ACATGT A^C[GA][CT]GT
AocI 334 CCTGAGG CC^T[ACGT]AGG
AviII 1348 TGCGCA TGC^GCA
AvrII 807 CCTAGG C^CTAGG
BamHI 768 GGATCC G^GATCC
BanI 1374 GGCGCC G^G[CT][GA]CC
BanII 330 GAGCCC G[GA]GC[CT]^C
BbeI 1374 GGCGCC GGCGC^C
BbsI 680 GAAGAC GAAGAC(2/6)
BbvCI 1369 GCTGAGG CCTCAGC(-5/-2)
BcgI 1463 GCAGGGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1246 GTATCC GTATCC(6/5)
BcnI 948 CCGGG CC^[GC]GG
BlnI 807 CCTAGG C^CTAGG
BlpI 246 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 246 GCTAAGC GC^T[ACGT]AGC
BpuAI 680 GAAGAC GAAGAC(2/6)
BsaHI 1374 GGCGCC G[GA]^CG[CT]C
BspLU11I 301 ACATGT A^CATGT
BspMI 523 ACCTGC ACCTGC(4/8)
BsrFI 638 GCCGGC [GA]^CCGG[CT]
BstAPI 852 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 708 GGTTACC G^GT[ACGT]ACC
BstPI 708 GGTTACC G^GT[ACGT]ACC
Bsu36I 334 CCTGAGG CC^T[ACGT]AGG
CelII 246 GCTAAGC GC^T[ACGT]AGC
Cfr10I 638 GCCGGC [GA]^CCGG[CT]
DraII 476 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 334 CCTGAGG CC^T[ACGT]AGG
EcoNI 1566 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 476 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 708 GGTTACC G^GT[ACGT]ACC
FspI 1348 TGCGCA TGC^GCA
HaeII 1374 GGCGCC [GA]GCGC^[CT]
HgaI 1258 GACGC GACGC(5/10)
Hin1I 1374 GGCGCC G[GA]^CG[CT]C
HincII 139 GTTGAC GT[CT]^[GA]AC
HindII 139 GTTGAC GT[CT]^[GA]AC
KasI 1374 GGCGCC G^GCGCC
NaeI 638 GCCGGC GCC^GGC
NarI 1374 GGCGCC GG^CGCC
NciI 948 CCGGG CC^[GC]GG
NgoMIV 638 GCCGGC G^CCGGC
PpuMI 476 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1501 CAGCTG CAG^CTG
ScaI 933 AGTACT AGT^ACT
SfoI 1374 GGCGCC GGC^GCC
SpeI 1476 ACTAGT A^CTAGT
TaqI 974 TCGA T^CGA
TthHB8I 974 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 519 GTCTAC GT^[AC][GT]AC
1255 GTAGAC
AciI 834 CCGC CCGC(-3/-1)
1668 CCGC
AfaI 934 GTAC GT^AC
1570 GTAC
AlwNI 1498 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1561 CAGCTCCTG
BalI 531 TGGCCA TGG^CCA
1526 TGGCCA
BsmFI 1467 GGGAC GGGAC(10/14)
199 GTCCC
HapII 639 CCGG C^CGG
948 CCGG
HpaII 639 CCGG C^CGG
948 CCGG
MluNI 531 TGGCCA TGG^CCA
1526 TGGCCA
MscI 531 TGGCCA TGG^CCA
1526 TGGCCA
MseI 356 TTAA T^TAA
745 TTAA
MspA1I 834 CCGCTG C[AC]G^C[GT]G
1501 CAGCTG
MspI 639 CCGG C^CGG
948 CCGG
NcoI 824 CCATGG C^CATGG
1241 CCATGG
NspI 301 ACATGT [GA]CATG^[CT]
1345 ACATGC
PstI 817 CTGCAG CTGCA^G
1282 CTGCAG
RsaI 934 GTAC GT^AC
1570 GTAC
SfaNI 635 GATGC GCATC(5/9)
391 GCATC
SfcI 817 CTGCAG C^T[GA][CT]AG
1282 CTGCAG
Tru9I 356 TTAA T^TAA
745 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
AspHI AvaII BbvI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
StyI TfiI TseI Tsp45I Tsp509I
TspRI XhoII XmnI