Restriction map for
pFN21AE6544
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI HpaII HphI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MspI MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AciI 41 GCGG CCGC(-3/-1)
AlwNI 115 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 144 GGGCCC GGGCC^C
AvaII 136 GGTCC G^G[AT]CC
BamHI 4 GGATCC G^GATCC
BanII 144 GGGCCC G[GA]GC[CT]^C
BciVI 249 GTATCC GTATCC(6/5)
BmyI 144 GGGCCC G[AGT]GC[ACT]^C
BpmI 218 CTGGAG CTGGAG(16/14)
BsiYI 166 CCTACAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 166 CCTACAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 144 GGGCCC G[AGT]GC[ACT]^C
BspHI 288 TCATGA T^CATGA
BspMI 209 ACCTGC ACCTGC(4/8)
BstYI 4 GGATCC [GA]^GATC[CT]
Cac8I 279 GCTGGC GC[ACGT]^[ACGT]GC
DdeI 259 CTCAG C^T[ACGT]AG
DpnII 5 GATC ^GATC
DraII 135 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 295 TGGCCG [CT]^GGCC[GA]
EarI 70 GAAGAG CTCTTC(1/4)
EcoO109I 135 GGGTCCT [GA]G^G[ACGT]CC[CT]
HgaI 29 GCGTC GACGC(5/10)
Ksp632I 70 GAAGAG CTCTTC(1/4)
MboI 5 GATC ^GATC
MboII 70 GAAGA GAAGA(8/7)
MflI 4 GGATCC [GA]^GATC[CT]
MseI 304 TTAA T^TAA
NdeII 5 GATC ^GATC
PpuMI 135 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 288 TCATGA T^CATGA
Sau3AI 5 GATC ^GATC
SfcI 167 CTACAG C^T[GA][CT]AG
Tru9I 304 TTAA T^TAA
Tsp45I 238 GTGAC ^GT[GC]AC
TspRI 115 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 131 CCAAGGGTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 4 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwI 5 GATCC GGATC(4/5)
4 GGATC
BsgI 13 GTGCAG GTGCAG(16/14)
90 GTGCAG
BsmFI 34 GGGAC GGGAC(10/14)
163 GTCCC
BsrI 293 ACTGG ACTGG(1/-1)
246 CCAGT
BstF5I 102 CATCC GGATG(2/0)
149 CATCC
EcoT14I 51 CCTTGG C^C[AT][AT]GG
131 CCAAGG
FokI 102 CATCC GGATG(9/13)
149 CATCC
MaeIII 176 GTTAC ^GT[ACGT]AC
238 GTGAC
MspA1I 170 CAGCTG C[AC]G^C[GT]G
277 CAGCTG
NlaIII 264 CATG CATG^
289 CATG
PvuII 170 CAGCTG CAG^CTG
277 CAGCTG
StyI 51 CCTTGG C^C[AT][AT]GG
131 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BbvI BsaJI BssKI BstNI
Cfr13I EcoRII Fnu4HI HaeIII ItaI
MnlI MvaI NlaIV Sau96I ScrFI
TseI