Restriction map for
pFN21AE5762
##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AlwNI AscI AseI
AsnI Asp718I AspEI AvrII BbvCI
BcgI BfrI BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI BspDI BspEI
BspLU11I BsrBI BssHII Bst1107I BstBI
BstXI BstZ17I CelII ClaI CpoI
DraI DrdI Eam1105I EcoRV EcoT22I
FseI HindIII HpaI KpnI MamI
MfeI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SbfI SexAI SfuI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 1885 GACGTC GACGT^C
AflIII 615 ACGCGT A^C[GA][CT]GT
Alw44I 601 GTGCAC G^TGCAC
Aor51HI 1157 AGCGCT AGC^GCT
ApaI 2057 GGGCCC GGGCC^C
ApaLI 601 GTGCAC G^TGCAC
AspI 1885 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1277 TGCGCA TGC^GCA
BbeI 2083 GGCGCC GGCGC^C
BbrPI 1240 CACGTG CAC^GTG
BbsI 1110 GAAGAC GAAGAC(2/6)
BciVI 840 GTATCC GTATCC(6/5)
BclI 1856 TGATCA T^GATCA
BfaI 64 CTAG C^TAG
BglI 2101 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1110 GAAGAC GAAGAC(2/6)
BsaAI 1240 CACGTG [CT]AC^GT[GA]
BsaI 1778 GAGACC GGTCTC(1/5)
BsgI 1744 CTGCAC GTGCAG(16/14)
BsiEI 1976 CGGCCG CG[GA][CT]^CG
BsmBI 982 GAGACG CGTCTC(1/5)
BsmI 318 GAATGC GAATGC(1/-1)
Bsp1407I 1760 TGTACA T^GTACA
BspHI 1896 TCATGA T^CATGA
BsrDI 131 GCAATG GCAATG(2/0)
BsrGI 1760 TGTACA T^GTACA
BstAPI 171 GCACACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 1976 CGGCCG C^GGCCG
EclXI 1976 CGGCCG C^GGCCG
Eco47III 1157 AGCGCT AGC^GCT
Eco52I 1976 CGGCCG C^GGCCG
EcoNI 1647 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 265 GAATTC G^AATTC
FbaI 1856 TGATCA T^GATCA
FspI 1277 TGCGCA TGC^GCA
HgaI 614 GACGC GACGC(5/10)
HincII 715 GTCAAC GT[CT]^[GA]AC
HindII 715 GTCAAC GT[CT]^[GA]AC
KasI 2083 GGCGCC G^GCGCC
KspI 1192 CCGCGG CCGC^GG
MaeI 64 CTAG C^TAG
MluI 615 ACGCGT A^CGCGT
NarI 2083 GGCGCC GG^CGCC
NcoI 2075 CCATGG C^CATGG
PaeR7I 367 CTCGAG C^TCGAG
PflFI 1885 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 436 GACTC GAGTC(4/5)
PmaCI 1240 CACGTG CAC^GTG
PmlI 1240 CACGTG CAC^GTG
RcaI 1896 TCATGA T^CATGA
SacI 1045 GAGCTC GAGCT^C
SacII 1192 CCGCGG CCGC^GG
SapI 87 GAAGAGC GCTCTTC(1/4)
SfiI 2100 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2083 GGCGCC GGC^GCC
SgrAI 1468 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 1760 TGTACA T^GTACA
Tth111I 1885 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 367 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 265 GAATTC [GA]^AATT[CT]
487 GAATTT
AcyI 1885 GACGTC G[GA]^CG[CT]C
2083 GGCGCC
AocI 1144 CCTGAGG CC^T[ACGT]AGG
2021 CCTCAGG
ApoI 265 GAATTC [GA]^AATT[CT]
487 GAATTT
Asp700I 87 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2121 GAACCTCTTC
BglII 1997 AGATCT A^GATCT
2471 AGATCT
BsaHI 1885 GACGTC G[GA]^CG[CT]C
2083 GGCGCC
BsrFI 98 ACCGGC [GA]^CCGG[CT]
1469 GCCGGT
BssSI 1714 CACGAG CACGAG(-5/-1)
190 CTCGTG
BstEII 1837 GGTTACC G^GT[ACGT]ACC
1869 GGTCACC
BstPI 1837 GGTTACC G^GT[ACGT]ACC
1869 GGTCACC
Bsu36I 1144 CCTGAGG CC^T[ACGT]AGG
2021 CCTCAGG
Cfr10I 98 ACCGGC [GA]^CCGG[CT]
1469 GCCGGT
Eco81I 1144 CCTGAGG CC^T[ACGT]AGG
2021 CCTCAGG
EcoO65I 1837 GGTTACC G^GT[ACGT]ACC
1869 GGTCACC
Hin1I 1885 GACGTC G[GA]^CG[CT]C
2083 GGCGCC
MseI 500 TTAA T^TAA
1406 TTAA
PflMI 186 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2384 CCAAGGAGTGG
ScaI 890 AGTACT AGT^ACT
2192 AGTACT
SmaI 1491 CCCGGG CCC^GGG
2522 CCCGGG
SmlI 367 CTCGAG C^T[CT][GA]AG
1630 CTCAAG
TfiI 476 GAATC G^A[AT]TC
1769 GAATC
Tru9I 500 TTAA T^TAA
1406 TTAA
Van91I 186 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2384 CCAAGGAGTGG
XmaCI 1491 CCCGGG C^CCGGG
2522 CCCGGG
XmaI 1491 CCCGGG C^CCGGG
2522 CCCGGG
XmnI 87 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2121 GAACCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII