Restriction map for
pFN21AE5827
##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BbrPI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 979 GACGTC GACGT^C
AccI 339 GTCGAC GT^[AC][GT]AC
AccIII 239 TCCGGA T^CCGGA
AcsI 1045 AAATTC [GA]^AATT[CT]
AhdI 349 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 476 AGCGCT AGC^GCT
ApaI 2014 GGGCCC GGGCC^C
ApoI 1045 AAATTC [GA]^AATT[CT]
AspEI 349 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1408 GGATCC G^GATCC
BbvCI 1930 GCTGAGG CCTCAGC(-5/-2)
BciVI 618 GGATAC GTATCC(6/5)
BglI 2007 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 54 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1620 GGTCTC GGTCTC(1/5)
BsaWI 239 TCCGGA [AT]^CCGG[AT]
BseAI 239 TCCGGA T^CCGGA
BspEI 239 TCCGGA T^CCGGA
BsrDI 2120 GCAATG GCAATG(2/0)
BstXI 800 CCAAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1946 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 349 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 476 AGCGCT AGC^GCT
KspI 2303 CCGCGG CCGC^GG
MamI 54 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 239 TCCGGA T^CCGGA
MseI 2723 TTAA T^TAA
MslI 483 CACCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 390 CCATGG C^CATGG
NruI 2546 TCGCGA TCG^CGA
PflMI 120 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1659 GAGCTC GAGCT^C
SacII 2303 CCGCGG CCGC^GG
SalI 339 GTCGAC G^TCGAC
SapI 945 GCTCTTC GCTCTTC(1/4)
SexAI 431 ACCTGGT A^CC[AT]GGT
SmaI 1580 CCCGGG CCC^GGG
Tru9I 2723 TTAA T^TAA
Tsp509I 1046 AATT ^AATT
Van91I 120 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 834 TCTAGA T^CTAGA
XmaCI 1580 CCCGGG C^CCGGG
XmaI 1580 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 609 GTAC GT^AC
2682 GTAC
AlwNI 163 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1679 CAGCTACTG
AocI 984 CCTGAGG CC^T[ACGT]AGG
1726 CCTGAGG
Asp700I 3 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2328 GAAGTCATTC
BalI 491 TGGCCA TGG^CCA
1293 TGGCCA
BbeI 1970 GGCGCC GGCGC^C
2062 GGCGCC
BbsI 696 GAAGAC GAAGAC(2/6)
1036 GAAGAC
BcgI 2011 CGAGGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1904 GCAGCATGATCG
BpuAI 696 GAAGAC GAAGAC(2/6)
1036 GAAGAC
BsmBI 1201 GAGACG CGTCTC(1/5)
1915 GAGACG
BsrBI 1358 CCGCTC CCGCTC(-3/-3)
2175 GAGCGG
BssSI 232 CACGAG CACGAG(-5/-1)
268 CTCGTG
BstAPI 23 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
510 GCACACCCTGC
Bsu36I 984 CCTGAGG CC^T[ACGT]AGG
1726 CCTGAGG
Eco81I 984 CCTGAGG CC^T[ACGT]AGG
1726 CCTGAGG
HgaI 1977 GACGC GACGC(5/10)
2618 GCGTC
HincII 339 GTCGAC GT[CT]^[GA]AC
2569 GTCAAC
HindII 339 GTCGAC GT[CT]^[GA]AC
2569 GTCAAC
KasI 1970 GGCGCC G^GCGCC
2062 GGCGCC
MaeII 785 ACGT A^CGT
980 ACGT
MluNI 491 TGGCCA TGG^CCA
1293 TGGCCA
MscI 491 TGGCCA TGG^CCA
1293 TGGCCA
NarI 1970 GGCGCC GG^CGCC
2062 GGCGCC
RsaI 609 GTAC GT^AC
2682 GTAC
SfoI 1970 GGCGCC GGC^GCC
2062 GGCGCC
StuI 196 AGGCCT AGG^CCT
669 AGGCCT
XmnI 3 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2328 GAAGTCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BglII BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XhoII