Restriction map for
pFN21AE5661
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI Aor51HI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BbeI BbsI BcgI BclI
BfrI BglII BlnI BpuAI BsaWI
BseAI BsiWI BspDI BspEI BspLU11I
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI DraIII DrdI Eco47III
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI HincII
HindII HpaI KasI KpnI MfeI
MluI MroI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StyI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AhdI 568 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1093 GTGCAC G^TGCAC
AocI 113 CCTCAGG CC^T[ACGT]AGG
ApaI 1384 GGGCCC GGGCC^C
ApaLI 1093 GTGCAC G^TGCAC
Asp700I 813 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 568 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 209 TGGCCA TGG^CCA
BbrPI 205 CACGTG CAC^GTG
BbvCI 177 GCTGAGG CCTCAGC(-5/-2)
BciVI 1229 GTATCC GTATCC(6/5)
BfaI 370 CTAG C^TAG
BlpI 1509 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1509 GCTGAGC GC^T[ACGT]AGC
BsaAI 205 CACGTG [CT]AC^GT[GA]
BsaBI 357 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 598 GAGACC GGTCTC(1/5)
BsgI 973 CTGCAC GTGCAG(16/14)
BsiEI 1466 CGGCCG CG[GA][CT]^CG
BsmBI 1087 GAGACG CGTCTC(1/5)
BsmFI 1347 GGGAC GGGAC(10/14)
BsmI 1169 GCATTC GAATGC(1/-1)
Bsp1407I 1040 TGTACA T^GTACA
BspHI 680 TCATGA T^CATGA
BsrDI 1242 CATTGC GCAATG(2/0)
BsrGI 1040 TGTACA T^GTACA
BssHII 1450 GCGCGC G^CGCGC
BstEII 403 GGTAACC G^GT[ACGT]ACC
BstPI 403 GGTAACC G^GT[ACGT]ACC
Bsu36I 113 CCTCAGG CC^T[ACGT]AGG
CelII 1509 GCTGAGC GC^T[ACGT]AGC
EagI 1466 CGGCCG C^GGCCG
Eam1105I 568 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1466 CGGCCG C^GGCCG
Eco52I 1466 CGGCCG C^GGCCG
Eco81I 113 CCTCAGG CC^T[ACGT]AGG
EcoO65I 403 GGTAACC G^GT[ACGT]ACC
HaeII 419 AGCGCC [GA]GCGC^[CT]
HindIII 76 AAGCTT A^AGCTT
HinfI 1066 GAGTC G^A[ACGT]TC
KspI 603 CCGCGG CCGC^GG
MaeI 370 CTAG C^TAG
MamI 357 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 209 TGGCCA TGG^CCA
MscI 209 TGGCCA TGG^CCA
MslI 858 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 499 GCCGGC GCC^GGC
NgoMIV 499 GCCGGC G^CCGGC
NspI 953 GCATGT [GA]CATG^[CT]
PleI 1066 GAGTC GAGTC(4/5)
PmaCI 205 CACGTG CAC^GTG
PmlI 205 CACGTG CAC^GTG
PvuII 485 CAGCTG CAG^CTG
RcaI 680 TCATGA T^CATGA
SacII 603 CCGCGG CCGC^GG
ScaI 581 AGTACT AGT^ACT
SexAI 737 ACCTGGT A^CC[AT]GGT
SfcI 219 CTACAG C^T[GA][CT]AG
SmlI 1537 CTCAAG C^T[CT][GA]AG
SspBI 1040 TGTACA T^GTACA
StuI 87 AGGCCT AGG^CCT
TspRI 107 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 813 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AatII 573 GACGTC GACGT^C
847 GACGTC
AcyI 573 GACGTC G[GA]^CG[CT]C
847 GACGTC
AlwI 546 GGATC GGATC(4/5)
1154 GGATC
AvaI 304 CCCGAG C^[CT]CG[GA]G
1027 CCCGAG
BanI 496 GGTGCC G^G[CT][GA]CC
1405 GGTGCC
BsaHI 573 GACGTC G[GA]^CG[CT]C
847 GACGTC
BseRI 781 GAGGAG GAGGAG(10/8)
1030 GAGGAG
BsoBI 304 CCCGAG C^[CT]CG[GA]G
1027 CCCGAG
BsrBI 1441 CCGCTC CCGCTC(-3/-3)
519 GAGCGG
BsrFI 499 GCCGGC [GA]^CCGG[CT]
587 ACCGGC
BssSI 39 CACGAG CACGAG(-5/-1)
289 CACGAG
BstYI 546 GGATCT [GA]^GATC[CT]
1154 GGATCT
Cfr10I 499 GCCGGC [GA]^CCGG[CT]
587 ACCGGC
DsaI 603 CCGCGG C^C[GA][CT]GG
1427 CCACGG
EarI 903 GAAGAG CTCTTC(1/4)
1302 GAAGAG
Hin1I 573 GACGTC G[GA]^CG[CT]C
847 GACGTC
Ksp632I 903 GAAGAG CTCTTC(1/4)
1302 GAAGAG
MflI 546 GGATCT [GA]^GATC[CT]
1154 GGATCT
PpuMI 214 AGGACCT [GA]G^G[AT]CC[CT]
1347 GGGACCC
XhoII 546 GGATCT [GA]^GATC[CT]
1154 GGATCT
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI ApoI AspHI AvaII BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I Tsp509I
TthHB8I