Restriction map for
pFN21AE5549
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BseAI BseRI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI Ksp632I KspI MamI MflI
MluI MluNI MroI MscI MslI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tsp45I Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AciI 477 CCGC CCGC(-3/-1)
AclI 814 AACGTT AA^CGTT
AfaI 574 GTAC GT^AC
ApaI 20 GGGCCC GGGCC^C
AseI 132 ATTAAT AT^TAAT
AsnI 132 ATTAAT AT^TAAT
AvaII 1137 GGACC G^G[AT]CC
BbsI 659 GTCTTC GAAGAC(2/6)
BcnI 1094 CCGGG CC^[GC]GG
BlpI 103 GCTAAGC GC^T[ACGT]AGC
BpmI 604 CTGGAG CTGGAG(16/14)
Bpu1102I 103 GCTAAGC GC^T[ACGT]AGC
BpuAI 659 GTCTTC GAAGAC(2/6)
BsaI 316 GAGACC GGTCTC(1/5)
BsaWI 878 TCCGGT [AT]^CCGG[AT]
BsgI 716 GTGCAG GTGCAG(16/14)
BsiEI 15 CGATCG CG[GA][CT]^CG
BsiYI 460 CCACAGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 460 CCACAGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 316 GAGAC GTCTC(1/5)
BsmFI 438 GTCCC GGGAC(10/14)
BsrDI 97 GCAATG GCAATG(2/0)
Cac8I 418 GCTCGC GC[ACGT]^[ACGT]GC
CelII 103 GCTAAGC GC^T[ACGT]AGC
DraIII 461 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 566 ATGCAT ATGCA^T
HincII 735 GTTGAC GT[CT]^[GA]AC
HindII 735 GTTGAC GT[CT]^[GA]AC
HphI 1102 GGTGA GGTGA(8/7)
MaeII 815 ACGT A^CGT
MfeI 481 CAATTG C^AATTG
MspA1I 1004 CAGCTG C[AC]G^C[GT]G
MunI 481 CAATTG C^AATTG
NciI 1094 CCGGG CC^[GC]GG
NsiI 566 ATGCAT ATGCA^T
PflMI 460 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 132 ATTAAT AT^TAAT
Psp1406I 814 AACGTT AA^CGTT
PvuI 15 CGATCG CGAT^CG
PvuII 1004 CAGCTG CAG^CTG
RsaI 574 GTAC GT^AC
TspRI 999 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 460 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 163 GAATTT [GA]^AATT[CT]
399 AAATTT
ApoI 163 GAATTT [GA]^AATT[CT]
399 AAATTT
Asp700I 241 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
793 GAAATTATTC
AspHI 47 GAGCTC G[AT]GC[AT]^C
416 GAGCTC
BfaI 56 CTAG C^TAG
823 CTAG
BsaJI 110 CCCAGG C^C[ACGT][ACGT]GG
1094 CCGGGG
BsiHKAI 47 GAGCTC G[AT]GC[AT]^C
416 GAGCTC
BsmI 153 GAATGC GAATGC(1/-1)
675 GAATGC
BsrI 890 ACTGG ACTGG(1/-1)
188 CCAGT
DraII 20 GGGCCCC [GA]G^G[ACGT]CC[CT]
37 AGGCCCC
EcoO109I 20 GGGCCCC [GA]G^G[ACGT]CC[CT]
37 AGGCCCC
HapII 879 CCGG C^CGG
1094 CCGG
HindIII 865 AAGCTT A^AGCTT
1154 AAGCTT
HpaII 879 CCGG C^CGG
1094 CCGG
MaeI 56 CTAG C^TAG
823 CTAG
MspI 879 CCGG C^CGG
1094 CCGG
NlaIII 565 CATG CATG^
904 CATG
PleI 91 GAGTC GAGTC(4/5)
899 GAGTC
PstI 834 CTGCAG CTGCA^G
1001 CTGCAG
SacI 47 GAGCTC GAGCT^C
416 GAGCTC
SfcI 834 CTGCAG C^T[GA][CT]AG
1001 CTGCAG
XmnI 241 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
793 GAAATTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI AlwI BanII BbvI BmyI
Bsp1286I BssKI BstF5I BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIV Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp509I TthHB8I