Restriction map for
pFN21AE6646
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
AscI AseI AsnI AspEI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FokI
FseI FspI Hin1I HindIII HinfI
HpaI KasI KspI MamI MfeI
MluI MroI MslI MspA1I MunI
MvnI MwoI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TaqI TfiI TthHB8I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 614 GGTACC G^GTACC
AcsI 438 AAATTT [GA]^AATT[CT]
AlwI 452 GATCC GGATC(4/5)
Aor51HI 589 AGCGCT AGC^GCT
ApoI 438 AAATTT [GA]^AATT[CT]
Asp700I 402 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 614 GGTACC G^GTACC
AspHI 484 GAGCAC G[AT]GC[AT]^C
AspI 627 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 201 GGACC G^G[AT]CC
BbvI 511 GCTGC GCAGC(8/12)
BfaI 523 CTAG C^TAG
BmyI 484 GAGCAC G[AGT]GC[ACT]^C
BpmI 371 CTCCAG CTGGAG(16/14)
BsaJI 324 CCCCGG C^C[ACGT][ACGT]GG
BsgI 356 GTGCAG GTGCAG(16/14)
BsiHKAI 484 GAGCAC G[AT]GC[AT]^C
BsmFI 200 GGGAC GGGAC(10/14)
Bsp1286I 484 GAGCAC G[AGT]GC[ACT]^C
BstNI 204 CCAGG CC^[AT]GG
BstYI 451 AGATCC [GA]^GATC[CT]
CfoI 590 GCGC GCG^C
Cfr13I 201 GGACC G^G[ACGT]CC
DdeI 32 CTAAG C^T[ACGT]AG
DpnII 452 GATC ^GATC
EarI 406 CTCTTC CTCTTC(1/4)
Eco47III 589 AGCGCT AGC^GCT
EcoRII 204 CCAGG ^CC[AT]GG
HaeII 589 AGCGCT [GA]GCGC^[CT]
HgaI 329 GACGC GACGC(5/10)
HhaI 590 GCGC GCG^C
HinP1I 590 GCGC G^CGC
HphI 641 TCACC GGTGA(8/7)
KpnI 614 GGTACC GGTAC^C
Ksp632I 406 CTCTTC CTCTTC(1/4)
MaeI 523 CTAG C^TAG
MboI 452 GATC ^GATC
MflI 451 AGATCC [GA]^GATC[CT]
MvaI 204 CCAGG CC^[AT]GG
NdeII 452 GATC ^GATC
PflFI 627 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 122 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 405 GCTCTTC GCTCTTC(1/4)
Sau3AI 452 GATC ^GATC
Sau96I 201 GGACC G^G[ACGT]CC
SfaNI 9 GCATC GCATC(5/9)
SspI 173 AATATT AAT^ATT
TseI 511 GCTGC G^C[AT]GC
Tth111I 627 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 122 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 373 CCAGCCTTTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 451 AGATCC [GA]^GATC[CT]
XmnI 402 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 367 CCGC CCGC(-3/-1)
229 GCGG
AluI 404 AGCT AG^CT
521 AGCT
BalI 98 TGGCCA TGG^CCA
414 TGGCCA
BanI 313 GGCACC G^G[CT][GA]CC
614 GGTACC
BcnI 325 CCCGG CC^[GC]GG
584 CCGGG
BsmI 220 GAATGC GAATGC(1/-1)
663 GAATGC
BspMI 3 GCAGGT ACCTGC(4/8)
358 GCAGGT
BsrI 103 ACTGG ACTGG(1/-1)
570 CCAGT
Cac8I 538 GCGAGC GC[ACGT]^[ACGT]GC
542 GCCTGC
EaeI 98 TGGCCA [CT]^GGCC[GA]
414 TGGCCA
Fnu4HI 366 GCCGC GC^[ACGT]GC
511 GCTGC
HapII 326 CCGG C^CGG
584 CCGG
HincII 277 GTCAAC GT[CT]^[GA]AC
624 GTTGAC
HindII 277 GTCAAC GT[CT]^[GA]AC
624 GTTGAC
HpaII 326 CCGG C^CGG
584 CCGG
ItaI 366 GCCGC GC^[ACGT]GC
511 GCTGC
MaeII 80 ACGT A^CGT
473 ACGT
MluNI 98 TGGCCA TGG^CCA
414 TGGCCA
MscI 98 TGGCCA TGG^CCA
414 TGGCCA
MseI 305 TTAA T^TAA
551 TTAA
MspI 326 CCGG C^CGG
584 CCGG
NciI 325 CCCGG CC^[GC]GG
584 CCGGG
Tru9I 305 TTAA T^TAA
551 TTAA
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AfaI BsiYI BslI BssKI HaeIII
MaeIII MboII MnlI NlaIV RsaI
ScrFI Tsp45I Tsp509I TspRI