Restriction map for
pFN21AE9686
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AflII AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaBI BsaI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 828 GTCTAC GT^[AC][GT]AC
AcyI 1492 GACGCC G[GA]^CG[CT]C
AfaI 1156 GTAC GT^AC
AflIII 641 ACGTGT A^C[GA][CT]GT
AgeI 1108 ACCGGT A^CCGGT
AvaI 734 CCCGGG C^[CT]CG[GA]G
AvaII 1093 GGTCC G^G[AT]CC
BamHI 216 GGATCC G^GATCC
BbsI 838 GAAGAC GAAGAC(2/6)
BpuAI 838 GAAGAC GAAGAC(2/6)
BsaAI 487 TACGTA [CT]AC^GT[GA]
BsaHI 1492 GACGCC G[GA]^CG[CT]C
BsaWI 1108 ACCGGT [AT]^CCGG[AT]
BseRI 1016 CTCCTC GAGGAG(10/8)
BsiYI 1549 CCTGGAATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1549 CCTGGAATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 377 GTCCC GGGAC(10/14)
BsoBI 734 CCCGGG C^[CT]CG[GA]G
BsrDI 1088 GCAATG GCAATG(2/0)
BstYI 216 GGATCC [GA]^GATC[CT]
Cfr13I 1093 GGTCC G^G[ACGT]CC
DraIII 1453 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 1048 GATATC GAT^ATC
HgaI 1492 GACGC GACGC(5/10)
Hin1I 1492 GACGCC G[GA]^CG[CT]C
HindIII 45 AAGCTT A^AGCTT
MflI 216 GGATCC [GA]^GATC[CT]
MslI 175 CACCTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 685 CATATG CA^TATG
NspI 892 GCATGT [GA]CATG^[CT]
PinAI 1108 ACCGGT A^CCGGT
RsaI 1156 GTAC GT^AC
Sau96I 1093 GGTCC G^G[ACGT]CC
SmaI 734 CCCGGG CCC^GGG
SmlI 1159 CTTGAG C^T[CT][GA]AG
SnaBI 487 TACGTA TAC^GTA
StuI 34 AGGCCT AGG^CCT
Tsp45I 303 GTCAC ^GT[GC]AC
XhoII 216 GGATCC [GA]^GATC[CT]
XmaCI 734 CCCGGG C^CCGGG
XmaI 734 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BalI 333 TGGCCA TGG^CCA
1508 TGGCCA
BanI 150 GGTGCC G^G[CT][GA]CC
173 GGCACC
BbvI 1482 GCAGC GCAGC(8/12)
1085 GCTGC
BcnI 734 CCCGG CC^[GC]GG
735 CCGGG
BsgI 1527 CTGCAC GTGCAG(16/14)
1559 GTGCAG
BspMI 114 ACCTGC ACCTGC(4/8)
225 GCAGGT
BsrI 1237 ACTGG ACTGG(1/-1)
269 CCAGT
BstAPI 109 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
979 GCAGATGATGC
Cac8I 127 GCCTGC GC[ACGT]^[ACGT]GC
1357 GCAGGC
EaeI 333 TGGCCA [CT]^GGCC[GA]
1508 TGGCCA
Fnu4HI 1085 GCTGC GC^[ACGT]GC
1482 GCAGC
ItaI 1085 GCTGC GC^[ACGT]GC
1482 GCAGC
MluNI 333 TGGCCA TGG^CCA
1508 TGGCCA
MscI 333 TGGCCA TGG^CCA
1508 TGGCCA
MspA1I 943 CAGCTG C[AC]G^C[GT]G
1483 CAGCTG
NciI 734 CCCGG CC^[GC]GG
735 CCGGG
PstI 977 CTGCAG CTGCA^G
1021 CTGCAG
PvuII 943 CAGCTG CAG^CTG
1483 CAGCTG
TseI 1085 GCTGC G^C[AT]GC
1482 GCAGC
TspRI 270 CAGTG CA[GC]TG[ACGT][ACGT]^
1562 CAGTG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AluI AlwI AlwNI ApoI
BfaI BsaJI BsmI BsrFI BssKI
BstF5I BstNI Cfr10I DdeI DpnII
DraI EarI EcoRII FokI HaeIII
HapII HinfI HpaII HphI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI ScrFI
SfaNI SfcI TfiI Tru9I Tsp509I