Restriction map for
pFN21AE6479
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AvaI AvaII
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrDI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HgaI HindIII HpaI KspI MfeI
MluI MluNI MroI MscI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TspRI Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 929 GGTACC G^GTACC
AccII 1392 CGCG CG^CG
AcyI 961 GGCGCC G[GA]^CG[CT]C
AlwNI 503 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 627 CCTCAGG CC^T[ACGT]AGG
ApaI 402 GGGCCC GGGCC^C
Asp718I 929 GGTACC G^GTACC
AspHI 817 GAGCAC G[AT]GC[AT]^C
AspI 565 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 961 GGCGCC GGCGC^C
BfaI 517 CTAG C^TAG
BpmI 319 CTGGAG CTGGAG(16/14)
BsaBI 34 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 961 GGCGCC G[GA]^CG[CT]C
BsiHKAI 817 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1127 TGTACA T^GTACA
BspMI 1065 GCAGGT ACCTGC(4/8)
BsrBI 226 CCGCTC CCGCTC(-3/-3)
BsrFI 412 GCCGGC [GA]^CCGG[CT]
BsrGI 1127 TGTACA T^GTACA
BssSI 1030 CTCGTG CACGAG(-5/-1)
BstUI 1392 CGCG CG^CG
Bsu36I 627 CCTCAGG CC^T[ACGT]AGG
Cfr10I 412 GCCGGC [GA]^CCGG[CT]
Eco81I 627 CCTCAGG CC^T[ACGT]AGG
EcoNI 1398 CCTGTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 187 GATATC GAT^ATC
FauI 225 CCCGC CCCGC(4/6)
HaeII 961 GGCGCC [GA]GCGC^[CT]
Hin1I 961 GGCGCC G[GA]^CG[CT]C
HincII 466 GTCAAC GT[CT]^[GA]AC
HindII 466 GTCAAC GT[CT]^[GA]AC
KasI 961 GGCGCC G^GCGCC
KpnI 929 GGTACC GGTAC^C
MaeI 517 CTAG C^TAG
MamI 34 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1392 CGCG CG^CG
NaeI 412 GCCGGC GCC^GGC
NarI 961 GGCGCC GG^CGCC
NcoI 948 CCATGG C^CATGG
NgoMIV 412 GCCGGC G^CCGGC
PflFI 565 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1162 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1414 CAGCTG CAG^CTG
SapI 1193 GAAGAGC GCTCTTC(1/4)
SfoI 961 GGCGCC GGC^GCC
SspBI 1127 TGTACA T^GTACA
Tsp509I 586 AATT ^AATT
Tth111I 565 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BbsI 273 GAAGAC GAAGAC(2/6)
1203 GAAGAC
BglI 482 GCCTCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
626 GCCTCAGGGGC
BpuAI 273 GAAGAC GAAGAC(2/6)
1203 GAAGAC
BsaI 1155 GGTCTC GGTCTC(1/5)
1407 GGTCTC
BsmBI 935 CGTCTC CGTCTC(1/5)
362 GAGACG
BsmFI 332 GGGAC GGGAC(10/14)
942 GTCCC
BsrI 180 ACTGG ACTGG(1/-1)
590 ACTGG
BstYI 221 AGATCC [GA]^GATC[CT]
881 AGATCC
CfoI 877 GCGC GCG^C
962 GCGC
DraII 401 AGGGCCC [GA]G^G[ACGT]CC[CT]
512 AGGGCCT
EcoO109I 401 AGGGCCC [GA]G^G[ACGT]CC[CT]
512 AGGGCCT
EcoT14I 863 CCAAGG C^C[AT][AT]GG
948 CCATGG
HhaI 877 GCGC GCG^C
962 GCGC
HinP1I 877 GCGC G^CGC
962 GCGC
MaeII 470 ACGT A^CGT
1292 ACGT
MflI 221 AGATCC [GA]^GATC[CT]
881 AGATCC
MspA1I 1316 CAGCGG C[AC]G^C[GT]G
1414 CAGCTG
PleI 1044 GACTC GAGTC(4/5)
499 GAGTC
PstI 146 CTGCAG CTGCA^G
311 CTGCAG
SfcI 146 CTGCAG C^T[GA][CT]AG
311 CTGCAG
StyI 863 CCAAGG C^C[AT][AT]GG
948 CCATGG
XhoII 221 AGATCC [GA]^GATC[CT]
881 AGATCC
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwI BanI
BanII BbvI BcnI BmyI BsaJI
BseRI BsgI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp45I
TthHB8I