Restriction map for
pFN21AE2581
##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AviII
AvrII BbeI BbrPI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BseAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI KpnI KspI MamI MluI
MroI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AatII 2825 GACGTC GACGT^C
AccI 2434 GTCTAC GT^[AC][GT]AC
AflIII 2430 ACACGT A^C[GA][CT]GT
AseI 1804 ATTAAT AT^TAAT
AsnI 1804 ATTAAT AT^TAAT
AvaI 132 CCCGAG C^[CT]CG[GA]G
BamHI 1956 GGATCC G^GATCC
BsaAI 655 TACGTG [CT]AC^GT[GA]
BsiWI 892 CGTACG C^GTACG
BsoBI 132 CCCGAG C^[CT]CG[GA]G
BspMI 612 ACCTGC ACCTGC(4/8)
BssSI 1334 CTCGTG CACGAG(-5/-1)
DrdI 1678 GACAGGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 1687 GTCAAC GT[CT]^[GA]AC
HindII 1687 GTCAAC GT[CT]^[GA]AC
MfeI 291 CAATTG C^AATTG
MunI 291 CAATTG C^AATTG
NaeI 1730 GCCGGC GCC^GGC
NcoI 2855 CCATGG C^CATGG
NgoMIV 1730 GCCGGC G^CCGGC
NspI 1724 ACATGC [GA]CATG^[CT]
PshBI 1804 ATTAAT AT^TAAT
SspI 2762 AATATT AAT^ATT
XcmI 560 CCAGAGAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Asp700I 1279 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1629 GAACACCTTC
AspHI 2036 GTGCTC G[AT]GC[AT]^C
2287 GAGCAC
AspI 931 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1311 GACAGAGTC
BbsI 2833 GAAGAC GAAGAC(2/6)
2940 GTCTTC
BbvCI 349 CCTCAGC CCTCAGC(-5/-2)
1525 CCTCAGC
BclI 1910 TGATCA T^GATCA
2963 TGATCA
BfaI 77 CTAG C^TAG
602 CTAG
BpuAI 2833 GAAGAC GAAGAC(2/6)
2940 GTCTTC
BsaWI 594 ACCGGA [AT]^CCGG[AT]
702 ACCGGA
BsgI 1108 GTGCAG GTGCAG(16/14)
1337 GTGCAG
BsiEI 807 CGGCCG CG[GA][CT]^CG
819 CGGCCG
BsiHKAI 2036 GTGCTC G[AT]GC[AT]^C
2287 GAGCAC
BsrBI 2061 CCGCTC CCGCTC(-3/-3)
2467 GAGCGG
BsrDI 167 CATTGC GCAATG(2/0)
2372 CATTGC
BsrFI 1730 GCCGGC [GA]^CCGG[CT]
2989 ACCGGC
Cfr10I 1730 GCCGGC [GA]^CCGG[CT]
2989 ACCGGC
DraIII 1102 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2884 CACTCAGTG
EagI 807 CGGCCG C^GGCCG
819 CGGCCG
EclXI 807 CGGCCG C^GGCCG
819 CGGCCG
Eco52I 807 CGGCCG C^GGCCG
819 CGGCCG
FbaI 1910 TGATCA T^GATCA
2963 TGATCA
MaeI 77 CTAG C^TAG
602 CTAG
MseI 1805 TTAA T^TAA
2462 TTAA
PflFI 931 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1311 GACAGAGTC
PpuMI 1832 AGGACCT [GA]G^G[AT]CC[CT]
2110 AGGACCC
SmlI 2148 CTCAAG C^T[CT][GA]AG
2407 CTCAAG
Tru9I 1805 TTAA T^TAA
2462 TTAA
Tth111I 931 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1311 GACAGAGTC
XmnI 1279 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1629 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AvaII
BalI BanI BanII BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII