Restriction map for
pFN21AE5360
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KspI MaeI
MaeIII MamI MfeI MluI MluNI
MscI MslI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp45I Tsp509I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 614 GGTACC G^GTACC
AccII 658 CGCG CG^CG
AccIII 299 TCCGGA T^CCGGA
AcyI 147 GGCGCC G[GA]^CG[CT]C
AlwI 564 GGATC GGATC(4/5)
AlwNI 121 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 140 GGGCCC GGGCC^C
Asp718I 614 GGTACC G^GTACC
AspHI 193 GAGCTC G[AT]GC[AT]^C
BbeI 147 GGCGCC GGCGC^C
BlpI 37 GCTCAGC GC^T[ACGT]AGC
BpmI 90 CTCCAG CTGGAG(16/14)
Bpu1102I 37 GCTCAGC GC^T[ACGT]AGC
BsaHI 147 GGCGCC G[GA]^CG[CT]C
BseAI 299 TCCGGA T^CCGGA
BsiHKAI 193 GAGCTC G[AT]GC[AT]^C
BsmAI 539 GAGAC GTCTC(1/5)
BsmBI 539 GAGACG CGTCTC(1/5)
BspEI 299 TCCGGA T^CCGGA
BspMI 173 ACCTGC ACCTGC(4/8)
BstUI 658 CGCG CG^CG
BstXI 251 CCAGCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 564 GGATCT [GA]^GATC[CT]
CelII 37 GCTCAGC GC^T[ACGT]AGC
DpnII 565 GATC ^GATC
DraII 521 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 606 GAAGAG CTCTTC(1/4)
EcoO109I 521 AGGACCC [GA]G^G[ACGT]CC[CT]
Hin1I 147 GGCGCC G[GA]^CG[CT]C
HinfI 226 GACTC G^A[ACGT]TC
HphI 626 TCACC GGTGA(8/7)
KasI 147 GGCGCC G^GCGCC
KpnI 614 GGTACC GGTAC^C
Ksp632I 606 GAAGAG CTCTTC(1/4)
MaeII 542 ACGT A^CGT
MboI 565 GATC ^GATC
MflI 564 GGATCT [GA]^GATC[CT]
MroI 299 TCCGGA T^CCGGA
MseI 764 TTAA T^TAA
MvnI 658 CGCG CG^CG
NaeI 374 GCCGGC GCC^GGC
NarI 147 GGCGCC GG^CGCC
NcoI 414 CCATGG C^CATGG
NdeII 565 GATC ^GATC
NgoMIV 374 GCCGGC G^CCGGC
PflMI 120 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 226 GACTC GAGTC(4/5)
PpuMI 521 AGGACCC [GA]G^G[AT]CC[CT]
PstI 493 CTGCAG CTGCA^G
SacI 193 GAGCTC GAGCT^C
Sau3AI 565 GATC ^GATC
SfaNI 294 GATGC GCATC(5/9)
SfcI 493 CTGCAG C^T[GA][CT]AG
SfoI 147 GGCGCC GGC^GCC
SmaI 143 CCCGGG CCC^GGG
TaqI 455 TCGA T^CGA
Tru9I 764 TTAA T^TAA
TthHB8I 455 TCGA T^CGA
Van91I 120 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 716 CCATGAACCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 564 GGATCT [GA]^GATC[CT]
XmaCI 143 CCCGGG C^CCGGG
XmaI 143 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 582 GTAC GT^AC
615 GTAC
AvaI 143 CCCGGG C^[CT]CG[GA]G
360 CCCGAG
BbsI 463 GAAGAC GAAGAC(2/6)
679 GTCTTC
BpuAI 463 GAAGAC GAAGAC(2/6)
679 GTCTTC
BsaWI 299 TCCGGA [AT]^CCGG[AT]
722 ACCGGA
BsgI 175 CTGCAC GTGCAG(16/14)
340 GTGCAG
BsiEI 372 CGGCCG CG[GA][CT]^CG
448 CGGCCG
BsmFI 56 GGGAC GGGAC(10/14)
434 GGGAC
BsoBI 143 CCCGGG C^[CT]CG[GA]G
360 CCCGAG
BsrBI 451 CCGCTC CCGCTC(-3/-3)
136 GAGCGG
BsrFI 374 GCCGGC [GA]^CCGG[CT]
395 ACCGGC
BssSI 742 CACGAG CACGAG(-5/-1)
473 CTCGTG
BstF5I 575 CATCC GGATG(2/0)
725 GGATG
BstNI 189 CCAGG CC^[AT]GG
578 CCAGG
Cfr10I 374 GCCGGC [GA]^CCGG[CT]
395 ACCGGC
DsaI 414 CCATGG C^C[GA][CT]GG
430 CCACGG
EaeI 372 CGGCCG [CT]^GGCC[GA]
448 CGGCCG
EagI 372 CGGCCG C^GGCCG
448 CGGCCG
EclXI 372 CGGCCG C^GGCCG
448 CGGCCG
Eco52I 372 CGGCCG C^GGCCG
448 CGGCCG
EcoRII 189 CCAGG ^CC[AT]GG
578 CCAGG
EcoT14I 414 CCATGG C^C[AT][AT]GG
686 CCAAGG
FokI 575 CATCC GGATG(9/13)
725 GGATG
MvaI 189 CCAGG CC^[AT]GG
578 CCAGG
NlaIII 415 CATG CATG^
717 CATG
RsaI 582 GTAC GT^AC
615 GTAC
StyI 414 CCATGG C^C[AT][AT]GG
686 CCAAGG
TspRI 346 CACTG CA[GC]TG[ACGT][ACGT]^
773 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AvaII BanI BanII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
Cac8I CfoI Cfr13I DdeI FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI MboII MnlI
MspA1I MspI MwoI NciI NlaIV
PvuII Sau96I ScrFI TseI