Restriction map for
pFN21AE2760
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfaI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DsaI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tru9I TspRI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccII 430 CGCG CG^CG
AspHI 328 GAGCAC G[AT]GC[AT]^C
BciVI 274 GTATCC GTATCC(6/5)
BglII 658 AGATCT A^GATCT
BpmI 686 CTGGAG CTGGAG(16/14)
BsgI 541 CTGCAC GTGCAG(16/14)
BsiEI 596 CGGCCG CG[GA][CT]^CG
BsiHKAI 328 GAGCAC G[AT]GC[AT]^C
BsmAI 41 GAGAC GTCTC(1/5)
BsmFI 149 GGGAC GGGAC(10/14)
BsmI 535 GCATTC GAATGC(1/-1)
Bsp1407I 482 TGTACA T^GTACA
BsrGI 482 TGTACA T^GTACA
BsrI 377 ACTGG ACTGG(1/-1)
BstAPI 498 GCAATTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 430 CGCG CG^CG
BstYI 658 AGATCT [GA]^GATC[CT]
DrdI 151 GACACAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 596 CGGCCG C^GGCCG
EclXI 596 CGGCCG C^GGCCG
Eco52I 596 CGGCCG C^GGCCG
EcoRI 316 GAATTC G^AATTC
EcoT14I 508 CCTTGG C^C[AT][AT]GG
EcoT22I 533 ATGCAT ATGCA^T
HaeII 399 GGCGCT [GA]GCGC^[CT]
MflI 658 AGATCT [GA]^GATC[CT]
MslI 331 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 235 CAGCTG C[AC]G^C[GT]G
MvnI 430 CGCG CG^CG
NsiI 533 ATGCAT ATGCA^T
PleI 414 GAGTC GAGTC(4/5)
PstI 405 CTGCAG CTGCA^G
PvuII 235 CAGCTG CAG^CTG
SspBI 482 TGTACA T^GTACA
StyI 508 CCTTGG C^C[AT][AT]GG
XhoII 658 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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BanII 425 GAGCCC G[GA]GC[CT]^C
689 GAGCCC
BbvI 237 GCTGC GCAGC(8/12)
611 GCTGC
BsiYI 285 CCCCTGCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
599 CCGCCATGCGG
BslI 285 CCCCTGCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
599 CCGCCATGCGG
BsrBI 132 CCGCTC CCGCTC(-3/-3)
28 GAGCGG
BstF5I 75 CATCC GGATG(2/0)
32 GGATG
CfoI 400 GCGC GCG^C
614 GCGC
EaeI 311 TGGCCG [CT]^GGCC[GA]
596 CGGCCG
FauI 362 CCCGC CCCGC(4/6)
428 CCCGC
FokI 75 CATCC GGATG(9/13)
32 GGATG
HhaI 400 GCGC GCG^C
614 GCGC
HinP1I 400 GCGC G^CGC
614 GCGC
HphI 263 TCACC GGTGA(8/7)
341 TCACC
MaeIII 340 GTCAC ^GT[ACGT]AC
669 GTCAC
SexAI 265 ACCTGGT A^CC[AT]GGT
625 ACCTGGT
SfcI 405 CTGCAG C^T[GA][CT]AG
662 CTACAG
TseI 237 GCTGC G^C[AT]GC
611 GCTGC
Tsp45I 340 GTCAC ^GT[GC]AC
669 GTCAC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BcnI BmyI BsaJI Bsp1286I
BssKI BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI HaeIII HapII
HinfI HpaII ItaI MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI TaqI TfiI Tsp509I
TthHB8I