Restriction map for
pFN21AE5169
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# NO CUT ENZYMES ( 197 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI Fnu4HI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HpaI HpaII ItaI
KasI KpnI Ksp632I KspI MaeI
MaeII MfeI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SapI Sau96I SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TseI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 368 GTCGAC GT^[AC][GT]AC
AciI 283 GCGG CCGC(-3/-1)
AcsI 157 GAATTT [GA]^AATT[CT]
ApoI 157 GAATTT [GA]^AATT[CT]
AseI 315 ATTAAT AT^TAAT
AsnI 315 ATTAAT AT^TAAT
AspHI 443 GAGCAC G[AT]GC[AT]^C
BamHI 63 GGATCC G^GATCC
BbsI 378 GAAGAC GAAGAC(2/6)
BglII 456 AGATCT A^GATCT
BmyI 443 GAGCAC G[AGT]GC[ACT]^C
BpmI 373 CTCCAG CTGGAG(16/14)
BpuAI 378 GAAGAC GAAGAC(2/6)
BsaBI 205 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 443 GAGCAC G[AT]GC[AT]^C
Bsp1286I 443 GAGCAC G[AGT]GC[ACT]^C
BssKI 72 CCAGG ^CC[ACGT]GG
BstNI 72 CCAGG CC^[AT]GG
BstXI 375 CCAGAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 18 GCTTGC GC[ACGT]^[ACGT]GC
EcoRII 72 CCAGG ^CC[AT]GG
HaeIII 257 GGCC GG^CC
HincII 368 GTCGAC GT[CT]^[GA]AC
HindII 368 GTCGAC GT[CT]^[GA]AC
HindIII 118 AAGCTT A^AGCTT
HphI 403 TCACC GGTGA(8/7)
MamI 205 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 316 TTAA T^TAA
MvaI 72 CCAGG CC^[AT]GG
MwoI 111 GCGAGAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 9 CATG CATG^
NlaIV 63 GGATCC GG[ACGT]^[ACGT]CC
PshBI 315 ATTAAT AT^TAAT
SalI 368 GTCGAC G^TCGAC
ScrFI 72 CCAGG CC^[ACGT]GG
SmlI 464 CTCAAG C^T[CT][GA]AG
TaqI 369 TCGA T^CGA
Tru9I 316 TTAA T^TAA
Tsp45I 12 GTCAC ^GT[GC]AC
TspRI 132 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 369 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AluI 17 AGCT AG^CT
119 AGCT
AlwI 64 GATCC GGATC(4/5)
63 GGATC
BsrI 224 ACTGG ACTGG(1/-1)
382 ACTGG
BstF5I 147 GGATG GGATG(2/0)
438 GGATG
BstYI 63 GGATCC [GA]^GATC[CT]
456 AGATCT
DdeI 121 CTTAG C^T[ACGT]AG
164 CTAAG
DpnII 64 GATC ^GATC
457 GATC
FokI 147 GGATG GGATG(9/13)
438 GGATG
MaeIII 12 GTCAC ^GT[ACGT]AC
394 GTAAC
MboI 64 GATC ^GATC
457 GATC
MboII 310 GAAGA GAAGA(8/7)
378 GAAGA
MflI 63 GGATCC [GA]^GATC[CT]
456 AGATCT
NdeII 64 GATC ^GATC
457 GATC
PleI 371 GACTC GAGTC(4/5)
462 GACTC
Sau3AI 64 GATC ^GATC
457 GATC
TfiI 138 GATTC G^A[AT]TC
228 GAATC
XhoII 63 GGATCC [GA]^GATC[CT]
456 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
HinfI MnlI Tsp509I