Restriction map for
pFN21AE6477
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BciVI BfrI BlnI BlpI Bpu1102I
BsaAI BsaI BsiEI BsiWI BsoBI
BspDI BspHI BspMI BsrDI BssHII
BstAPI BstBI BstXI CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tru9I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 1408 GACGTC GACGT^C
AccII 1215 CGCG CG^CG
AccIII 875 TCCGGA T^CCGGA
AcsI 552 GAATTC [GA]^AATT[CT]
AflIII 110 ACATGT A^C[GA][CT]GT
AlwNI 777 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 760 CCTGAGG CC^T[ACGT]AGG
ApaI 224 GGGCCC GGGCC^C
ApoI 552 GAATTC [GA]^AATT[CT]
AspHI 424 GAGCTC G[AT]GC[AT]^C
BamHI 878 GGATCC G^GATCC
BanI 1312 GGCACC G^G[CT][GA]CC
BbsI 1384 GTCTTC GAAGAC(2/6)
BbvCI 721 CCTCAGC CCTCAGC(-5/-2)
BclI 1181 TGATCA T^GATCA
BglII 1451 AGATCT A^GATCT
BpmI 45 CTCCAG CTGGAG(16/14)
BpuAI 1384 GTCTTC GAAGAC(2/6)
BsaBI 1176 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 875 TCCGGA [AT]^CCGG[AT]
BseAI 875 TCCGGA T^CCGGA
BseRI 719 CTCCTC GAGGAG(10/8)
BsiHKAI 424 GAGCTC G[AT]GC[AT]^C
BsmBI 1059 CGTCTC CGTCTC(1/5)
BsmI 362 GAATGC GAATGC(1/-1)
Bsp1407I 1133 TGTACA T^GTACA
BspEI 875 TCCGGA T^CCGGA
BspLU11I 110 ACATGT A^CATGT
BsrGI 1133 TGTACA T^GTACA
BssSI 421 CACGAG CACGAG(-5/-1)
Bst1107I 1441 GTATAC GTA^TAC
BstEII 492 GGTGACC G^GT[ACGT]ACC
BstPI 492 GGTGACC G^GT[ACGT]ACC
BstUI 1215 CGCG CG^CG
BstZ17I 1441 GTATAC GTA^TAC
Bsu36I 760 CCTGAGG CC^T[ACGT]AGG
DraIII 207 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 760 CCTGAGG CC^T[ACGT]AGG
EcoNI 41 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 492 GGTGACC G^GT[ACGT]ACC
EcoRI 552 GAATTC G^AATTC
FauI 528 GCGGG CCCGC(4/6)
FbaI 1181 TGATCA T^GATCA
HaeII 870 GGCGCT [GA]GCGC^[CT]
HgaI 665 GCGTC GACGC(5/10)
MaeII 1409 ACGT A^CGT
MamI 1176 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 875 TCCGGA T^CCGGA
MvnI 1215 CGCG CG^CG
NaeI 595 GCCGGC GCC^GGC
NgoMIV 595 GCCGGC G^CCGGC
SacI 424 GAGCTC GAGCT^C
SapI 841 GAAGAGC GCTCTTC(1/4)
SmlI 805 CTTGAG C^T[CT][GA]AG
SspBI 1133 TGTACA T^GTACA
StuI 66 AGGCCT AGG^CCT
XcmI 447 CCAGGTCTGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1008 GTCTAC GT^[AC][GT]AC
1441 GTATAC
AcyI 664 GGCGTC G[GA]^CG[CT]C
1408 GACGTC
AfaI 432 GTAC GT^AC
1134 GTAC
AlwI 879 GATCC GGATC(4/5)
878 GGATC
BalI 815 TGGCCA TGG^CCA
1146 TGGCCA
BcgI 840 CGAAGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
506 GCAACTGGTTCG
BcnI 19 CCGGG CC^[GC]GG
36 CCGGG
BsaHI 664 GGCGTC G[GA]^CG[CT]C
1408 GACGTC
BsgI 148 CTGCAC GTGCAG(16/14)
216 GTGCAG
BsrI 509 ACTGG ACTGG(1/-1)
947 ACTGG
BstYI 878 GGATCC [GA]^GATC[CT]
1451 AGATCT
EcoT14I 827 CCAAGG C^C[AT][AT]GG
989 CCAAGG
Hin1I 664 GGCGTC G[GA]^CG[CT]C
1408 GACGTC
MflI 878 GGATCC [GA]^GATC[CT]
1451 AGATCT
MluNI 815 TGGCCA TGG^CCA
1146 TGGCCA
MscI 815 TGGCCA TGG^CCA
1146 TGGCCA
NciI 19 CCGGG CC^[GC]GG
36 CCGGG
PflMI 473 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1367 CCACGGGCTGG
PpuMI 764 AGGACCC [GA]G^G[AT]CC[CT]
1001 GGGACCT
RsaI 432 GTAC GT^AC
1134 GTAC
StyI 827 CCAAGG C^C[AT][AT]GG
989 CCAAGG
Tsp45I 493 GTGAC ^GT[GC]AC
1294 GTCAC
Tsp509I 93 AATT ^AATT
553 AATT
Van91I 473 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1367 CCACGGGCTGG
XhoII 878 GGATCC [GA]^GATC[CT]
1451 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BfaI BglI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrFI BssKI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PvuII Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TspRI
TthHB8I