##################################
# NO CUT ENZYMES (  212 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AciI           64  GCGG                 CCGC(-3/-1)
AcsI          201  GAATTT               [GA]^AATT[CT]
AfaI          211  GTAC                 GT^AC
AlwI           90  GGATC                GGATC(4/5)
ApoI          201  GAATTT               [GA]^AATT[CT]
BanI           66  GGTGCC               G^G[CT][GA]CC
BbvI          132  GCAGC                GCAGC(8/12)
BglI          100  GCCTGTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmAI         240  GTCTC                GTCTC(1/5)
BspDI         126  ATCGAT               AT^CGAT
BssKI         148  CCTGG                ^CC[ACGT]GG
BstNI         148  CCTGG                CC^[AT]GG
ClaI          126  ATCGAT               AT^CGAT
DsaI          216  CCGTGG               C^C[GA][CT]GG
EcoRII        148  CCTGG                ^CC[AT]GG
Fnu4HI        132  GCAGC                GC^[ACGT]GC
HaeIII         39  GGCC                 GG^CC
HinfI         144  GAATC                G^A[ACGT]TC
ItaI          132  GCAGC                GC^[ACGT]GC
MboII         261  GAAGA                GAAGA(8/7)
MnlI          198  GAGG                 CCTC(7/6)
MseI          269  TTAA                 T^TAA
MvaI          148  CCTGG                CC^[AT]GG
MwoI          100  GCCTGTGTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
RsaI          211  GTAC                 GT^AC
ScrFI         148  CCTGG                CC^[ACGT]GG
StuI           38  AGGCCT               AGG^CCT
TaqI          127  TCGA                 T^CGA
TfiI          144  GAATC                G^A[AT]TC
Tru9I         269  TTAA                 T^TAA
TseI          132  GCAGC                G^C[AT]GC
Tsp509I       202  AATT                 ^AATT
TthHB8I       127  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BsaJI         148  CCTGGG               C^C[ACGT][ACGT]GG
              216  CCGTGG              
BsrI          188  ACTGG                ACTGG(1/-1)
              159  CCAGT               
DpnII          91  GATC                 ^GATC
              264  GATC                
HphI           21  GGTGA                GGTGA(8/7)
              220  GGTGA               
MboI           91  GATC                 ^GATC
              264  GATC                
MslI           11  CACAAGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              214  CACCGTGGTG          
NdeII          91  GATC                 ^GATC
              264  GATC                
NlaIV          66  GGTGCC               GG[ACGT]^[ACGT]CC
              155  GGAGCC              
Sau3AI         91  GATC                 ^GATC
              264  GATC                
SfaNI         129  GATGC                GCATC(5/9)
              124  GCATC               
TspRI          77  CAGTG                CA[GC]TG[ACGT][ACGT]^
              187  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

MaeIII      Tsp45I