Restriction map for
pFN21AE6291
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiWI BsmBI BsmFI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrDI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraI Eam1105I EarI Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HindIII HinfI
HpaI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuII RcaI
RsrII SacII SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 763 GTCGAC GT^[AC][GT]AC
AcyI 850 GGCGCC G[GA]^CG[CT]C
Asp700I 777 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 877 GAGCTC G[AT]GC[AT]^C
BanII 877 GAGCTC G[GA]GC[CT]^C
BbeI 850 GGCGCC GGCGC^C
BcgI 125 GCAAGCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 850 GGCGCC G[GA]^CG[CT]C
BsgI 541 GTGCAG GTGCAG(16/14)
BsiHKAI 877 GAGCTC G[AT]GC[AT]^C
Bsp1407I 749 TGTACA T^GTACA
BspMI 108 GCAGGT ACCTGC(4/8)
BsrBI 88 GAGCGG CCGCTC(-3/-3)
BsrGI 749 TGTACA T^GTACA
BstAPI 192 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 512 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 625 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 166 GACTGCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 512 GGGGCCT [GA]G^G[ACGT]CC[CT]
HaeII 850 GGCGCC [GA]GCGC^[CT]
Hin1I 850 GGCGCC G[GA]^CG[CT]C
HincII 763 GTCGAC GT[CT]^[GA]AC
HindII 763 GTCGAC GT[CT]^[GA]AC
KasI 850 GGCGCC G^GCGCC
MaeIII 658 GTGAC ^GT[ACGT]AC
NarI 850 GGCGCC GG^CGCC
NspI 599 GCATGC [GA]CATG^[CT]
PstI 106 CTGCAG CTGCA^G
PvuI 83 CGATCG CGAT^CG
SacI 877 GAGCTC GAGCT^C
SalI 763 GTCGAC G^TCGAC
SfcI 106 CTGCAG C^T[GA][CT]AG
SfoI 850 GGCGCC GGC^GCC
SphI 599 GCATGC GCATG^C
SspBI 749 TGTACA T^GTACA
Tsp45I 658 GTGAC ^GT[GC]AC
TspRI 531 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 777 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BanI 757 GGCACC G^G[CT][GA]CC
850 GGCGCC
BglII 290 AGATCT A^GATCT
779 AGATCT
BmyI 756 GGGCAC G[AGT]GC[ACT]^C
877 GAGCTC
BpmI 210 CTGGAG CTGGAG(16/14)
327 CTGGAG
BsmAI 473 GAGAC GTCTC(1/5)
535 GTCTC
Bsp1286I 756 GGGCAC G[AGT]GC[ACT]^C
877 GAGCTC
BsrFI 367 GCCGGC [GA]^CCGG[CT]
670 GCCGGC
BstYI 290 AGATCT [GA]^GATC[CT]
779 AGATCT
Cfr10I 367 GCCGGC [GA]^CCGG[CT]
670 GCCGGC
DsaI 74 CCGTGG C^C[GA][CT]GG
665 CCGTGG
EcoNI 206 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
516 CCTGTACCAGG
EcoT14I 185 CCAAGG C^C[AT][AT]GG
593 CCAAGG
MflI 290 AGATCT [GA]^GATC[CT]
779 AGATCT
MslI 627 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
711 CATGATGATG
MspA1I 397 CCGCTG C[AC]G^C[GT]G
425 CAGCGG
NaeI 367 GCCGGC GCC^GGC
670 GCCGGC
NgoMIV 367 GCCGGC G^CCGGC
670 GCCGGC
SmlI 251 CTCAAG C^T[CT][GA]AG
880 CTCAAG
StuI 465 AGGCCT AGG^CCT
803 AGGCCT
StyI 185 CCAAGG C^C[AT][AT]GG
593 CCAAGG
XhoII 290 AGATCT [GA]^GATC[CT]
779 AGATCT
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BbvI BcnI BsaJI BsiEI BsiYI
BslI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DpnII EaeI
EagI EclXI Eco52I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TthHB8I XcmI