Restriction map for
pFN21AE5784
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII BalI BamHI BbeI
BcgI BclI BglI BglII BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstYI BstZ17I Cfr10I
ClaI CpoI DrdI EaeI EagI
Eam1105I EclXI Eco52I EcoNI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HpaI KasI KpnI MamI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI SspI SwaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 2055 CGCG CG^CG
AflII 1414 CTTAAG C^TTAAG
AlwI 684 GATCC GGATC(4/5)
Aor51HI 1785 AGCGCT AGC^GCT
AspI 513 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2077 CCCGAG C^[CT]CG[GA]G
AvrII 394 CCTAGG C^CTAGG
BbrPI 905 CACGTG CAC^GTG
BbsI 2071 GAAGAC GAAGAC(2/6)
BciVI 523 GGATAC GTATCC(6/5)
BfrI 1414 CTTAAG C^TTAAG
BlnI 394 CCTAGG C^CTAGG
BlpI 1711 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1711 GCTAAGC GC^T[ACGT]AGC
BpuAI 2071 GAAGAC GAAGAC(2/6)
BsaAI 905 CACGTG [CT]AC^GT[GA]
BseRI 1546 CTCCTC GAGGAG(10/8)
BsmBI 1197 CGTCTC CGTCTC(1/5)
BsoBI 2077 CCCGAG C^[CT]CG[GA]G
BspHI 1931 TCATGA T^CATGA
BspMI 662 ACCTGC ACCTGC(4/8)
BsrDI 281 CATTGC GCAATG(2/0)
BstAPI 1225 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 55 TTCGAA TT^CGAA
BstEII 1381 GGTCACC G^GT[ACGT]ACC
BstPI 1381 GGTCACC G^GT[ACGT]ACC
BstUI 2055 CGCG CG^CG
BstXI 954 CCAGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1711 GCTAAGC GC^T[ACGT]AGC
DraI 301 TTTAAA TTT^AAA
DsaI 2054 CCGCGG C^C[GA][CT]GG
Eco47III 1785 AGCGCT AGC^GCT
EcoO65I 1381 GGTCACC G^GT[ACGT]ACC
EcoRI 259 GAATTC G^AATTC
FauI 1881 GCGGG CCCGC(4/6)
HaeII 1785 AGCGCT [GA]GCGC^[CT]
HincII 613 GTTGAC GT[CT]^[GA]AC
HindII 613 GTTGAC GT[CT]^[GA]AC
KspI 2054 CCGCGG CCGC^GG
MaeII 906 ACGT A^CGT
MslI 1791 CACGGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 2055 CGCG CG^CG
NspV 55 TTCGAA TT^CGAA
PflFI 513 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 905 CACGTG CAC^GTG
PmlI 905 CACGTG CAC^GTG
RcaI 1931 TCATGA T^CATGA
SacII 2054 CCGCGG CCGC^GG
SapI 1926 GCTCTTC GCTCTTC(1/4)
SbfI 1020 CCTGCAGG CCTGCA^GG
SfuI 55 TTCGAA TT^CGAA
SphI 1988 GCATGC GCATG^C
Sse8387I 1020 CCTGCAGG CCTGCA^GG
StuI 795 AGGCCT AGG^CCT
TaqI 56 TCGA T^CGA
Tth111I 513 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 56 TCGA T^CGA
XbaI 186 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 149 AAATTT [GA]^AATT[CT]
259 GAATTC
AflIII 488 ACATGT A^C[GA][CT]GT
1628 ACATGT
ApoI 149 AAATTT [GA]^AATT[CT]
259 GAATTC
BanI 745 GGCACC G^G[CT][GA]CC
1578 GGCACC
BcnI 890 CCCGG CC^[GC]GG
1978 CCGGG
BsaI 914 GAGACC GGTCTC(1/5)
1102 GAGACC
BsmI 865 GCATTC GAATGC(1/-1)
1160 GCATTC
BspLU11I 488 ACATGT A^CATGT
1628 ACATGT
CfoI 1786 GCGC GCG^C
1872 GCGC
HapII 891 CCGG C^CGG
1978 CCGG
HhaI 1786 GCGC GCG^C
1872 GCGC
HinP1I 1786 GCGC G^CGC
1872 GCGC
HindIII 606 AAGCTT A^AGCTT
1714 AAGCTT
HpaII 891 CCGG C^CGG
1978 CCGG
MspI 891 CCGG C^CGG
1978 CCGG
NciI 890 CCCGG CC^[GC]GG
1978 CCGGG
PpuMI 1836 GGGACCC [GA]G^G[AT]CC[CT]
1949 AGGTCCT
SacI 1089 GAGCTC GAGCT^C
1543 GAGCTC
SexAI 1172 ACCTGGT A^CC[AT]GGT
1967 ACCAGGT
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwNI AocI
Asp700I AspHI AvaII BanII BbvCI
BbvI BfaI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
Cfr13I DdeI DpnII DraII DraIII
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XmnI