Restriction map for
pFN21AE6548
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AlwI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BmyI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Cfr10I Cfr13I
ClaI CpoI DpnII DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MslI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI Sau3AI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AfaI 243 GTAC GT^AC
AlwNI 179 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 299 CCTCAGG CC^T[ACGT]AGG
BbsI 45 GAAGAC GAAGAC(2/6)
BbvCI 289 CCTCAGC CCTCAGC(-5/-2)
BlpI 187 GCTGAGC GC^T[ACGT]AGC
BpmI 55 CTCCAG CTGGAG(16/14)
Bpu1102I 187 GCTGAGC GC^T[ACGT]AGC
BpuAI 45 GAAGAC GAAGAC(2/6)
BsaAI 240 CACGTA [CT]AC^GT[GA]
BseRI 297 CTCCTC GAGGAG(10/8)
BspHI 228 TCATGA T^CATGA
BspMI 218 ACCTGC ACCTGC(4/8)
BsrI 178 CCAGT ACTGG(1/-1)
BstF5I 284 GGATG GGATG(2/0)
Bsu36I 299 CCTCAGG CC^T[ACGT]AGG
CelII 187 GCTGAGC GC^T[ACGT]AGC
CfoI 40 GCGC GCG^C
EarI 117 GAAGAG CTCTTC(1/4)
Eco81I 299 CCTCAGG CC^T[ACGT]AGG
EcoRI 141 GAATTC G^AATTC
FokI 284 GGATG GGATG(9/13)
HaeII 39 GGCGCT [GA]GCGC^[CT]
HaeIII 304 GGCC GG^CC
HhaI 40 GCGC GCG^C
HinP1I 40 GCGC G^CGC
HphI 313 TCACC GGTGA(8/7)
Ksp632I 117 GAAGAG CTCTTC(1/4)
MaeII 241 ACGT A^CGT
MaeIII 312 GTCAC ^GT[ACGT]AC
MwoI 183 GCCTGCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 229 CATG CATG^
PleI 310 GAGTC GAGTC(4/5)
PstI 220 CTGCAG CTGCA^G
RcaI 228 TCATGA T^CATGA
RsaI 243 GTAC GT^AC
TfiI 109 GAATC G^A[AT]TC
Tsp45I 312 GTCAC ^GT[GC]AC
TspRI 179 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 21 GCGG CCGC(-3/-1)
106 GCGG
AcsI 141 GAATTC [GA]^AATT[CT]
277 AAATTC
ApoI 141 GAATTC [GA]^AATT[CT]
277 AAATTC
BcnI 35 CCGGG CC^[GC]GG
306 CCGGG
BsaJI 35 CCGGGG C^C[ACGT][ACGT]GG
65 CCCAGG
BsiYI 295 CCCTCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
299 CCTCAGGCCGG
BslI 295 CCCTCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
299 CCTCAGGCCGG
BstNI 57 CCAGG CC^[AT]GG
66 CCAGG
Cac8I 171 GCCAGC GC[ACGT]^[ACGT]GC
183 GCCTGC
EcoRII 57 CCAGG ^CC[AT]GG
66 CCAGG
HapII 35 CCGG C^CGG
306 CCGG
HinfI 109 GAATC G^A[ACGT]TC
310 GAGTC
HpaII 35 CCGG C^CGG
306 CCGG
MboII 45 GAAGA GAAGA(8/7)
117 GAAGA
MspI 35 CCGG C^CGG
306 CCGG
MvaI 57 CCAGG CC^[AT]GG
66 CCAGG
NciI 35 CCGGG CC^[GC]GG
306 CCGGG
SfaNI 285 GATGC GCATC(5/9)
225 GCATC
SfcI 220 CTGCAG C^T[GA][CT]AG
234 CTACAG
Tsp509I 142 AATT ^AATT
278 AATT
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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BbvI BssKI DdeI Fnu4HI ItaI
MnlI ScrFI TaqI TseI TthHB8I