Restriction map for
pFN21AE5676
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcnI BfaI BfrI
BglI BlnI BsaAI BsaHI BsaI
BsgI BsiEI BsiWI BsmAI BsmBI
BsoBI BspDI BspHI BspMI BsrBI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MfeI
MluI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 7 CGCG CG^CG
AccIII 87 TCCGGA T^CCGGA
AlwNI 617 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 643 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 910 GGACC G^G[AT]CC
BalI 1591 TGGCCA TGG^CCA
BanII 28 GGGCTC G[GA]GC[CT]^C
BcgI 690 CGAAGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1213 GGATAC GTATCC(6/5)
BclI 1269 TGATCA T^GATCA
BlpI 21 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 21 GCTCAGC GC^T[ACGT]AGC
BsaBI 1234 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 87 TCCGGA [AT]^CCGG[AT]
BseAI 87 TCCGGA T^CCGGA
BseRI 808 GAGGAG GAGGAG(10/8)
BsiYI 570 CCTTAGTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 570 CCTTAGTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 973 GAATGC GAATGC(1/-1)
Bsp1407I 1108 TGTACA T^GTACA
BspEI 87 TCCGGA T^CCGGA
BsrFI 889 GCCGGT [GA]^CCGG[CT]
BsrGI 1108 TGTACA T^GTACA
BstBI 688 TTCGAA TT^CGAA
BstUI 7 CGCG CG^CG
CelII 21 GCTCAGC GC^T[ACGT]AGC
Cfr10I 889 GCCGGT [GA]^CCGG[CT]
EaeI 1591 TGGCCA [CT]^GGCC[GA]
EcoT14I 282 CCAAGG C^C[AT][AT]GG
FbaI 1269 TGATCA T^GATCA
HaeII 1550 GGCGCT [GA]GCGC^[CT]
HgaI 8 GCGTC GACGC(5/10)
MamI 1234 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1591 TGGCCA TGG^CCA
MroI 87 TCCGGA T^CCGGA
MscI 1591 TGGCCA TGG^CCA
MspA1I 24 CAGCGG C[AC]G^C[GT]G
MvnI 7 CGCG CG^CG
NspV 688 TTCGAA TT^CGAA
PstI 681 CTGCAG CTGCA^G
SfuI 688 TTCGAA TT^CGAA
SspBI 1108 TGTACA T^GTACA
SspI 989 AATATT AAT^ATT
StuI 958 AGGCCT AGG^CCT
StyI 282 CCAAGG C^C[AT][AT]GG
TaqI 689 TCGA T^CGA
TthHB8I 689 TCGA T^CGA
XmnI 643 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AspHI 511 GAGCAC G[AT]GC[AT]^C
612 GTGCTC
BpmI 1454 CTCCAG CTGGAG(16/14)
388 CTGGAG
BsaJI 282 CCAAGG C^C[ACGT][ACGT]GG
386 CCCTGG
BsiHKAI 511 GAGCAC G[AT]GC[AT]^C
612 GTGCTC
BsmFI 823 GGGAC GGGAC(10/14)
723 GTCCC
BssKI 387 CCTGG ^CC[ACGT]GG
1486 CCTGG
BstNI 387 CCTGG CC^[AT]GG
1486 CCTGG
BstXI 524 CCATAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1582 CCAATGAGATGG
CfoI 6 GCGC GCG^C
1551 GCGC
Cfr13I 910 GGACC G^G[ACGT]CC
1141 GGCCC
DraI 493 TTTAAA TTT^AAA
883 TTTAAA
DraIII 1218 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
1220 CACAGTGTG
EcoRII 387 CCTGG ^CC[AT]GG
1486 CCTGG
FauI 26 GCGGG CCCGC(4/6)
35 GCGGG
HapII 88 CCGG C^CGG
890 CCGG
HhaI 6 GCGC GCG^C
1551 GCGC
HinP1I 6 GCGC G^CGC
1551 GCGC
HpaII 88 CCGG C^CGG
890 CCGG
MspI 88 CCGG C^CGG
890 CCGG
MvaI 387 CCTGG CC^[AT]GG
1486 CCTGG
Sau96I 910 GGACC G^G[ACGT]CC
1141 GGCCC
ScrFI 387 CCTGG CC^[ACGT]GG
1486 CCTGG
Tsp45I 815 GTGAC ^GT[GC]AC
1430 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI BbsI BbvI BglII
BmyI BpuAI Bsp1286I BspLU11I BsrDI
BsrI BstF5I BstYI Cac8I DdeI
DpnII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MflI MnlI MseI MslI MwoI
NdeII NlaIII NspI PleI RsaI
Sau3AI SfaNI SfcI TfiI Tru9I
TseI Tsp509I TspRI XhoII