Restriction map for
pFN21AE6556
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BglII BlnI BmyI BpmI
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FokI FseI FspI
HapII HgaI Hin1I HincII HindII
HpaI HpaII HphI KasI KpnI
Ksp632I KspI MaeI MaeII MfeI
MflI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NlaIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tth111I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 357 GAATTT [GA]^AATT[CT]
AfaI 443 GTAC GT^AC
AhdI 288 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 349 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 357 GAATTT [GA]^AATT[CT]
AspEI 288 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 209 GTCTTC GAAGAC(2/6)
BclI 387 TGATCA T^GATCA
BlpI 402 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 402 GCTGAGC GC^T[ACGT]AGC
BpuAI 209 GTCTTC GAAGAC(2/6)
BsaBI 319 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
CelII 402 GCTGAGC GC^T[ACGT]AGC
CfoI 108 GCGC GCG^C
Cfr13I 379 GGCCC G^G[ACGT]CC
DsaI 424 CCATGG C^C[GA][CT]GG
Eam1105I 288 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 424 CCATGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
FbaI 387 TGATCA T^GATCA
HaeII 107 GGCGCT [GA]GCGC^[CT]
HhaI 108 GCGC GCG^C
HinP1I 108 GCGC G^CGC
HindIII 342 AAGCTT A^AGCTT
HinfI 310 GACTC G^A[ACGT]TC
MamI 319 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 210 TCTTC GAAGA(8/7)
MnlI 300 GAGG CCTC(7/6)
NcoI 424 CCATGG C^CATGG
NsiI 1 ATGCAT ATGCA^T
NspI 220 GCATGC [GA]CATG^[CT]
PflMI 278 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 310 GACTC GAGTC(4/5)
RsaI 443 GTAC GT^AC
Sau96I 379 GGCCC G^G[ACGT]CC
SfaNI 319 GATGC GCATC(5/9)
SphI 220 GCATGC GCATG^C
StyI 424 CCATGG C^C[AT][AT]GG
Tsp45I 308 GTGAC ^GT[GC]AC
TspRI 391 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 278 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 382 CCATCTGATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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BbvI 160 GCTGC GCAGC(8/12)
217 GCTGC
BsaJI 57 CCCAGG C^C[ACGT][ACGT]GG
424 CCATGG
BsiYI 98 CCTTAATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
278 CCAAGAACTGG
BslI 98 CCTTAATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
278 CCAAGAACTGG
BsmI 69 GCATTC GAATGC(1/-1)
81 GCATTC
BsrI 284 ACTGG ACTGG(1/-1)
392 ACTGG
BssKI 58 CCAGG ^CC[ACGT]GG
348 CCAGG
BstNI 58 CCAGG CC^[AT]GG
348 CCAGG
BstXI 226 CCAGAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
424 CCATGGAAATGG
Cac8I 48 GCCTGC GC[ACGT]^[ACGT]GC
220 GCATGC
DdeI 332 CTCAG C^T[ACGT]AG
403 CTGAG
DpnII 325 GATC ^GATC
388 GATC
EcoRII 58 CCAGG ^CC[AT]GG
348 CCAGG
Fnu4HI 160 GCTGC GC^[ACGT]GC
217 GCTGC
HaeIII 379 GGCC GG^CC
418 GGCC
ItaI 160 GCTGC GC^[ACGT]GC
217 GCTGC
MaeIII 11 GTAAC ^GT[ACGT]AC
308 GTGAC
MboI 325 GATC ^GATC
388 GATC
MslI 200 CATCAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
425 CATGGAAATG
MvaI 58 CCAGG CC^[AT]GG
348 CCAGG
NdeII 325 GATC ^GATC
388 GATC
Sau3AI 325 GATC ^GATC
388 GATC
ScrFI 58 CCAGG CC^[ACGT]GG
348 CCAGG
TseI 160 GCTGC G^C[AT]GC
217 GCTGC
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# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
AluI MseI MwoI NlaIII Tru9I
Tsp509I