Restriction map for
pFN21AE6383
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HapII
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MslI
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 832 AAATTT [GA]^AATT[CT]
AfaI 553 GTAC GT^AC
ApoI 832 AAATTT [GA]^AATT[CT]
BalI 492 TGGCCA TGG^CCA
BanI 682 GGTGCC G^G[CT][GA]CC
BciVI 804 GGATAC GTATCC(6/5)
BfaI 928 CTAG C^TAG
BsaAI 631 TACGTG [CT]AC^GT[GA]
BsgI 711 CTGCAC GTGCAG(16/14)
BsiEI 66 CGGCCG CG[GA][CT]^CG
BsmFI 202 GGGAC GGGAC(10/14)
BsmI 777 GAATGC GAATGC(1/-1)
BspMI 122 GCAGGT ACCTGC(4/8)
BstF5I 826 GGATG GGATG(2/0)
BstYI 719 AGATCC [GA]^GATC[CT]
CfoI 19 GCGC GCG^C
DraIII 668 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 800 CCATGG C^C[GA][CT]GG
EagI 66 CGGCCG C^GGCCG
EclXI 66 CGGCCG C^GGCCG
Eco52I 66 CGGCCG C^GGCCG
EcoT14I 800 CCATGG C^C[AT][AT]GG
FauI 102 CCCGC CCCGC(4/6)
FokI 826 GGATG GGATG(9/13)
HhaI 19 GCGC GCG^C
HinP1I 19 GCGC G^CGC
HphI 211 GGTGA GGTGA(8/7)
MaeI 928 CTAG C^TAG
MflI 719 AGATCC [GA]^GATC[CT]
MluNI 492 TGGCCA TGG^CCA
MscI 492 TGGCCA TGG^CCA
NcoI 800 CCATGG C^CATGG
NotI 65 GCGGCCGC GC^GGCCGC
PflMI 664 CCATCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 288 GAGTC GAGTC(4/5)
PstI 562 CTGCAG CTGCA^G
RsaI 553 GTAC GT^AC
SfaNI 676 GCATC GCATC(5/9)
SfcI 562 CTGCAG C^T[GA][CT]AG
SmlI 603 CTCAAG C^T[CT][GA]AG
StyI 800 CCATGG C^C[AT][AT]GG
Tsp45I 505 GTGAC ^GT[GC]AC
Van91I 664 CCATCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 791 CCATTATCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 719 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AspHI 82 GAGCTC G[AT]GC[AT]^C
863 GAGCTC
BanII 82 GAGCTC G[GA]GC[CT]^C
863 GAGCTC
BclI 575 TGATCA T^GATCA
840 TGATCA
BmyI 82 GAGCTC G[AGT]GC[ACT]^C
863 GAGCTC
BseRI 112 CTCCTC GAGGAG(10/8)
79 GAGGAG
BsiHKAI 82 GAGCTC G[AT]GC[AT]^C
863 GAGCTC
BsmAI 446 GAGAC GTCTC(1/5)
290 GTCTC
Bsp1286I 82 GAGCTC G[AGT]GC[ACT]^C
863 GAGCTC
BspHI 689 TCATGA T^CATGA
897 TCATGA
BstAPI 676 GCATCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
772 GCAGGGAATGC
Cac8I 494 GCCAGC GC[ACGT]^[ACGT]GC
735 GCTTGC
FbaI 575 TGATCA T^GATCA
840 TGATCA
HgaI 119 GACGC GACGC(5/10)
55 GCGTC
MaeII 632 ACGT A^CGT
763 ACGT
MseI 128 TTAA T^TAA
464 TTAA
RcaI 689 TCATGA T^CATGA
897 TCATGA
SacI 82 GAGCTC GAGCT^C
863 GAGCTC
TaqI 186 TCGA T^CGA
592 TCGA
Tru9I 128 TTAA T^TAA
464 TTAA
Tsp509I 619 AATT ^AATT
833 AATT
TspRI 87 CACTG CA[GC]TG[ACGT][ACGT]^
368 CACTG
TthHB8I 186 TCGA T^CGA
592 TCGA
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BsaJI BsiYI BslI BssKI BstNI
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI HaeIII HinfI ItaI MaeIII
MboI MboII MnlI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PvuII
Sau3AI Sau96I ScrFI TfiI TseI