################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TfiI Tru9I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AlwI 369 GGATC GGATC(4/5) AlwNI 124 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 358 GAGCAC G[AT]GC[AT]^C BbsI 208 GTCTTC GAAGAC(2/6) BbvI 39 GCTGC GCAGC(8/12) BlpI 42 GCTGAGC GC^T[ACGT]AGC BmyI 358 GAGCAC G[AGT]GC[ACT]^C Bpu1102I 42 GCTGAGC GC^T[ACGT]AGC BpuAI 208 GTCTTC GAAGAC(2/6) BsaI 175 GAGACC GGTCTC(1/5) BsiHKAI 358 GAGCAC G[AT]GC[AT]^C BsmAI 175 GAGAC GTCTC(1/5) BsmFI 93 GTCCC GGGAC(10/14) Bsp1286I 358 GAGCAC G[AGT]GC[ACT]^C BspMI 249 GCAGGT ACCTGC(4/8) BssSI 361 CACGAG CACGAG(-5/-1) BstXI 185 CCACCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 42 GCTGAGC GC^T[ACGT]AGC CfoI 341 GCGC GCG^C FauI 204 GCGGG CCCGC(4/6) HaeII 340 AGCGCC [GA]GCGC^[CT] HaeIII 157 GGCC GG^CC HapII 198 CCGG C^CGG HhaI 341 GCGC GCG^C HinP1I 341 GCGC G^CGC HpaII 198 CCGG C^CGG MaeII 71 ACGT A^CGT MspI 198 CCGG C^CGG PpuMI 91 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 6 GACTTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SspI 235 AATATT AAT^ATT TseI 39 GCTGC G^C[AT]GC Tsp509I 266 AATT ^AATT TspRI 162 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AvaII 92 GGTCC G^G[AT]CC 195 GGTCC BstF5I 117 CATCC GGATG(2/0) 273 GGATG DdeI 43 CTGAG C^T[ACGT]AG 160 CTCAG DpnII 303 GATC ^GATC 370 GATC DraII 91 GGGTCCC [GA]G^G[ACGT]CC[CT] 155 AGGGCCT EcoO109I 91 GGGTCCC [GA]G^G[ACGT]CC[CT] 155 AGGGCCT FokI 117 CATCC GGATG(9/13) 273 GGATG MboI 303 GATC ^GATC 370 GATC MslI 30 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 186 CACCAGGGTG MspA1I 19 CAGCTG C[AC]G^C[GT]G 60 CAGCTG MwoI 33 GCTGGTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 343 GCCGATGCCGC NdeII 303 GATC ^GATC 370 GATC NlaIV 91 GGGTCC GG[ACGT]^[ACGT]CC 92 GGTCCC PflMI 18 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 123 CCAGGAGCTGG PvuII 19 CAGCTG CAG^CTG 60 CAGCTG Sau3AI 303 GATC ^GATC 370 GATC SfaNI 75 GATGC GCATC(5/9) 346 GATGC TaqI 321 TCGA T^CGA 379 TCGA TthHB8I 321 TCGA T^CGA 379 TCGA Van91I 18 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 123 CCAGGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AciI AluI BsaJI BseRI BsiYI BslI BssKI BstNI Cfr13I EcoRII Fnu4HI HphI ItaI MaeIII MboII MnlI MvaI Sau96I ScrFI Tsp45I