Restriction map for
pFN21AE6580
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BpmI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I Cac8I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MseI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI TfiI
Tru9I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AlwI 369 GGATC GGATC(4/5)
AlwNI 124 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 358 GAGCAC G[AT]GC[AT]^C
BbsI 208 GTCTTC GAAGAC(2/6)
BbvI 39 GCTGC GCAGC(8/12)
BlpI 42 GCTGAGC GC^T[ACGT]AGC
BmyI 358 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 42 GCTGAGC GC^T[ACGT]AGC
BpuAI 208 GTCTTC GAAGAC(2/6)
BsaI 175 GAGACC GGTCTC(1/5)
BsiHKAI 358 GAGCAC G[AT]GC[AT]^C
BsmAI 175 GAGAC GTCTC(1/5)
BsmFI 93 GTCCC GGGAC(10/14)
Bsp1286I 358 GAGCAC G[AGT]GC[ACT]^C
BspMI 249 GCAGGT ACCTGC(4/8)
BssSI 361 CACGAG CACGAG(-5/-1)
BstXI 185 CCACCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 42 GCTGAGC GC^T[ACGT]AGC
CfoI 341 GCGC GCG^C
FauI 204 GCGGG CCCGC(4/6)
HaeII 340 AGCGCC [GA]GCGC^[CT]
HaeIII 157 GGCC GG^CC
HapII 198 CCGG C^CGG
HhaI 341 GCGC GCG^C
HinP1I 341 GCGC G^CGC
HpaII 198 CCGG C^CGG
MaeII 71 ACGT A^CGT
MspI 198 CCGG C^CGG
PpuMI 91 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 6 GACTTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SspI 235 AATATT AAT^ATT
TseI 39 GCTGC G^C[AT]GC
Tsp509I 266 AATT ^AATT
TspRI 162 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 20 enzymes) #
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AvaII 92 GGTCC G^G[AT]CC
195 GGTCC
BstF5I 117 CATCC GGATG(2/0)
273 GGATG
DdeI 43 CTGAG C^T[ACGT]AG
160 CTCAG
DpnII 303 GATC ^GATC
370 GATC
DraII 91 GGGTCCC [GA]G^G[ACGT]CC[CT]
155 AGGGCCT
EcoO109I 91 GGGTCCC [GA]G^G[ACGT]CC[CT]
155 AGGGCCT
FokI 117 CATCC GGATG(9/13)
273 GGATG
MboI 303 GATC ^GATC
370 GATC
MslI 30 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
186 CACCAGGGTG
MspA1I 19 CAGCTG C[AC]G^C[GT]G
60 CAGCTG
MwoI 33 GCTGGTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
343 GCCGATGCCGC
NdeII 303 GATC ^GATC
370 GATC
NlaIV 91 GGGTCC GG[ACGT]^[ACGT]CC
92 GGTCCC
PflMI 18 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
123 CCAGGAGCTGG
PvuII 19 CAGCTG CAG^CTG
60 CAGCTG
Sau3AI 303 GATC ^GATC
370 GATC
SfaNI 75 GATGC GCATC(5/9)
346 GATGC
TaqI 321 TCGA T^CGA
379 TCGA
TthHB8I 321 TCGA T^CGA
379 TCGA
Van91I 18 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
123 CCAGGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI BsaJI BseRI BsiYI
BslI BssKI BstNI Cfr13I EcoRII
Fnu4HI HphI ItaI MaeIII MboII
MnlI MvaI Sau96I ScrFI Tsp45I