##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       BalI        BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BfrI        BglI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        Fnu4HI      FokI        FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HphI        ItaI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MunI        MvnI        
MwoI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SwaI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     Tth111I     TthHB8I     XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AciI          163  CCGC                 CCGC(-3/-1)
AlwNI         347  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          319  GGGCCC               GGGCC^C
AspHI         280  GAGCTC               G[AT]GC[AT]^C
AvrII         295  CCTAGG               C^CTAGG
BamHI          24  GGATCC               G^GATCC
BglII         376  AGATCT               A^GATCT
BlnI          295  CCTAGG               C^CTAGG
BpmI          389  CTCCAG               CTGGAG(16/14)
BsaWI          66  TCCGGT               [AT]^CCGG[AT]
BseRI          31  CTCCTC               GAGGAG(10/8)
BsgI          151  GTGCAG               GTGCAG(16/14)
BsiHKAI       280  GAGCTC               G[AT]GC[AT]^C
BsmAI         229  GAGAC                GTCTC(1/5)
BsmFI         343  GTCCC                GGGAC(10/14)
BsrBI         163  CCGCTC               CCGCTC(-3/-3)
BstXI         139  CCAAGGAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          113  GCGC                 GCG^C
EarI          277  GAAGAG               CTCTTC(1/4)
HhaI          113  GCGC                 GCG^C
HinP1I        113  GCGC                 G^CGC
Ksp632I       277  GAAGAG               CTCTTC(1/4)
MaeIII        103  GTTAC                ^GT[ACGT]AC
NcoI          417  CCATGG               C^CATGG
NlaIII        418  CATG                 CATG^
PflMI         346  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI          280  GAGCTC               GAGCT^C
SapI          277  GAAGAGC              GCTCTTC(1/4)
SspI          169  AATATT               AAT^ATT
StuI          286  AGGCCT               AGG^CCT
TspRI          99  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        346  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AfaI           70  GTAC                 GT^AC
              426  GTAC                
AvaII         120  GGACC                G^G[AT]CC
              342  GGTCC               
BanI          218  GGCACC               G^G[CT][GA]CC
              413  GGTGCC              
BanII         280  GAGCTC               G[GA]GC[CT]^C
              319  GGGCCC              
BcnI            8  CCCGG                CC^[GC]GG
               36  CCGGG               
BmyI          280  GAGCTC               G[AGT]GC[ACT]^C
              319  GGGCCC              
Bsp1286I      280  GAGCTC               G[AGT]GC[ACT]^C
              319  GGGCCC              
DsaI          116  CCGTGG               C^C[GA][CT]GG
              417  CCATGG              
HinfI          63  GATTC                G^A[ACGT]TC
              190  GATTC               
MboII         277  GAAGA                GAAGA(8/7)
              374  GAAGA               
NciI            8  CCCGG                CC^[GC]GG
               36  CCGGG               
RsaI           70  GTAC                 GT^AC
              426  GTAC                
TfiI           63  GATTC                G^A[AT]TC
              190  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AluI        AlwI        BfaI        BsaJI       BsiYI       
BslI        BssKI       BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      EcoT14I     HaeIII      HapII       HpaII       
MaeI        MboI        MflI        MnlI        MspI        
MvaI        NdeII       NlaIV       Sau3AI      Sau96I      
ScrFI       StyI        XhoII