################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AhdI AlwI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BsaI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 145 CGCG CG^CG AgeI 274 ACCGGT A^CCGGT Alw44I 73 GTGCAC G^TGCAC AlwNI 184 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 73 GTGCAC G^TGCAC AspHI 73 GTGCAC G[AT]GC[AT]^C AvaI 512 CCCGGG C^[CT]CG[GA]G BbrPI 76 CACGTG CAC^GTG BclI 318 TGATCA T^GATCA BsaAI 76 CACGTG [CT]AC^GT[GA] BsaBI 444 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 73 GTGCAC G[AT]GC[AT]^C BsoBI 512 CCCGGG C^[CT]CG[GA]G BspMI 518 ACCTGC ACCTGC(4/8) BsrDI 543 CATTGC GCAATG(2/0) BsrFI 274 ACCGGT [GA]^CCGG[CT] BssSI 454 CTCGTG CACGAG(-5/-1) BstEII 270 GGTGACC G^GT[ACGT]ACC BstPI 270 GGTGACC G^GT[ACGT]ACC BstUI 145 CGCG CG^CG Cfr10I 274 ACCGGT [GA]^CCGG[CT] DpnII 319 GATC ^GATC DraII 515 GGGACCT [GA]G^G[ACGT]CC[CT] EaeI 236 CGGCCA [CT]^GGCC[GA] EcoNI 345 CCTGAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 515 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 270 GGTGACC G^GT[ACGT]ACC FbaI 318 TGATCA T^GATCA HaeII 435 GGCGCT [GA]GCGC^[CT] HincII 37 GTCAAC GT[CT]^[GA]AC HindII 37 GTCAAC GT[CT]^[GA]AC MaeII 77 ACGT A^CGT MamI 444 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 319 GATC ^GATC MvnI 145 CGCG CG^CG MwoI 127 GCTAACTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 65 CCATGG C^CATGG NdeII 319 GATC ^GATC PinAI 274 ACCGGT A^CCGGT PleI 59 GAGTC GAGTC(4/5) PmaCI 76 CACGTG CAC^GTG PmlI 76 CACGTG CAC^GTG PpuMI 515 GGGACCT [GA]G^G[AT]CC[CT] Sau3AI 319 GATC ^GATC SexAI 585 ACCTGGT A^CC[AT]GGT SmaI 512 CCCGGG CCC^GGG TfiI 635 GATTC G^A[AT]TC Tsp509I 627 AATT ^AATT TspRI 419 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 246 CCACTTTTCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 512 CCCGGG C^CCGGG XmaI 512 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BbsI 565 GTCTTC GAAGAC(2/6) 590 GTCTTC BbvI 112 GCAGC GCAGC(8/12) 208 GCTGC BpuAI 565 GTCTTC GAAGAC(2/6) 590 GTCTTC BsaWI 165 TCCGGT [AT]^CCGG[AT] 274 ACCGGT BsmAI 224 GTCTC GTCTC(1/5) 383 GTCTC CfoI 136 GCGC GCG^C 436 GCGC DsaI 65 CCATGG C^C[GA][CT]GG 611 CCACGG EarI 226 CTCTTC CTCTTC(1/4) 385 CTCTTC FauI 527 CCCGC CCCGC(4/6) 336 GCGGG HhaI 136 GCGC GCG^C 436 GCGC HinP1I 136 GCGC G^CGC 436 GCGC HinfI 59 GAGTC G^A[ACGT]TC 635 GATTC Ksp632I 226 CTCTTC CTCTTC(1/4) 385 CTCTTC SfcI 219 CTACAG C^T[GA][CT]AG 287 CTACAG TseI 112 GCAGC G^C[AT]GC 208 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AluI AvaII BanII BcnI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MboII MnlI MslI MspA1I MspI MvaI NciI NlaIII NlaIV PvuII Sau96I ScrFI SfaNI StyI Tsp45I