Restriction map for
pFN21AE6294
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AhdI AlwI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbvCI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsaI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BsrI BssHII Bst1107I BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 145 CGCG CG^CG
AgeI 274 ACCGGT A^CCGGT
Alw44I 73 GTGCAC G^TGCAC
AlwNI 184 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 73 GTGCAC G^TGCAC
AspHI 73 GTGCAC G[AT]GC[AT]^C
AvaI 512 CCCGGG C^[CT]CG[GA]G
BbrPI 76 CACGTG CAC^GTG
BclI 318 TGATCA T^GATCA
BsaAI 76 CACGTG [CT]AC^GT[GA]
BsaBI 444 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 73 GTGCAC G[AT]GC[AT]^C
BsoBI 512 CCCGGG C^[CT]CG[GA]G
BspMI 518 ACCTGC ACCTGC(4/8)
BsrDI 543 CATTGC GCAATG(2/0)
BsrFI 274 ACCGGT [GA]^CCGG[CT]
BssSI 454 CTCGTG CACGAG(-5/-1)
BstEII 270 GGTGACC G^GT[ACGT]ACC
BstPI 270 GGTGACC G^GT[ACGT]ACC
BstUI 145 CGCG CG^CG
Cfr10I 274 ACCGGT [GA]^CCGG[CT]
DpnII 319 GATC ^GATC
DraII 515 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 236 CGGCCA [CT]^GGCC[GA]
EcoNI 345 CCTGAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 515 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 270 GGTGACC G^GT[ACGT]ACC
FbaI 318 TGATCA T^GATCA
HaeII 435 GGCGCT [GA]GCGC^[CT]
HincII 37 GTCAAC GT[CT]^[GA]AC
HindII 37 GTCAAC GT[CT]^[GA]AC
MaeII 77 ACGT A^CGT
MamI 444 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 319 GATC ^GATC
MvnI 145 CGCG CG^CG
MwoI 127 GCTAACTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 65 CCATGG C^CATGG
NdeII 319 GATC ^GATC
PinAI 274 ACCGGT A^CCGGT
PleI 59 GAGTC GAGTC(4/5)
PmaCI 76 CACGTG CAC^GTG
PmlI 76 CACGTG CAC^GTG
PpuMI 515 GGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 319 GATC ^GATC
SexAI 585 ACCTGGT A^CC[AT]GGT
SmaI 512 CCCGGG CCC^GGG
TfiI 635 GATTC G^A[AT]TC
Tsp509I 627 AATT ^AATT
TspRI 419 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 246 CCACTTTTCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 512 CCCGGG C^CCGGG
XmaI 512 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BbsI 565 GTCTTC GAAGAC(2/6)
590 GTCTTC
BbvI 112 GCAGC GCAGC(8/12)
208 GCTGC
BpuAI 565 GTCTTC GAAGAC(2/6)
590 GTCTTC
BsaWI 165 TCCGGT [AT]^CCGG[AT]
274 ACCGGT
BsmAI 224 GTCTC GTCTC(1/5)
383 GTCTC
CfoI 136 GCGC GCG^C
436 GCGC
DsaI 65 CCATGG C^C[GA][CT]GG
611 CCACGG
EarI 226 CTCTTC CTCTTC(1/4)
385 CTCTTC
FauI 527 CCCGC CCCGC(4/6)
336 GCGGG
HhaI 136 GCGC GCG^C
436 GCGC
HinP1I 136 GCGC G^CGC
436 GCGC
HinfI 59 GAGTC G^A[ACGT]TC
635 GATTC
Ksp632I 226 CTCTTC CTCTTC(1/4)
385 CTCTTC
SfcI 219 CTACAG C^T[GA][CT]AG
287 CTACAG
TseI 112 GCAGC G^C[AT]GC
208 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AvaII BanII BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeIII MboII MnlI MslI
MspA1I MspI MvaI NciI NlaIII
NlaIV PvuII Sau96I ScrFI SfaNI
StyI Tsp45I