Restriction map for
pFN21AE2753
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI AluI Alw44I AlwNI
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DdeI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeIII HapII HincII HindII HindIII
HinfI HpaI HpaII KasI KpnI
Ksp632I KspI MaeI MaeII MaeIII
MfeI MluI MluNI MroI MscI
MseI MslI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tsp509I TspRI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AciI 40 CCGC CCGC(-3/-1)
AcyI 31 GACGCC G[GA]^CG[CT]C
AlwI 60 GATCC GGATC(4/5)
Aor51HI 149 AGCGCT AGC^GCT
BanII 169 GAGCCC G[GA]GC[CT]^C
BbvI 137 GCTGC GCAGC(8/12)
BglII 11 AGATCT A^GATCT
BmyI 169 GAGCCC G[AGT]GC[ACT]^C
BsaBI 54 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 31 GACGCC G[GA]^CG[CT]C
BseRI 166 GAGGAG GAGGAG(10/8)
BsmFI 26 GGGAC GGGAC(10/14)
Bsp1286I 169 GAGCCC G[AGT]GC[ACT]^C
BspMI 191 ACCTGC ACCTGC(4/8)
BstAPI 8 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 150 GCGC GCG^C
DraII 188 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 161 CCATGG C^C[GA][CT]GG
Eco47III 149 AGCGCT AGC^GCT
EcoO109I 188 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 39 CCCGC CCCGC(4/6)
Fnu4HI 137 GCTGC GC^[ACGT]GC
HaeII 149 AGCGCT [GA]GCGC^[CT]
HgaI 31 GACGC GACGC(5/10)
HhaI 150 GCGC GCG^C
Hin1I 31 GACGCC G[GA]^CG[CT]C
HinP1I 150 GCGC G^CGC
ItaI 137 GCTGC GC^[ACGT]GC
MamI 54 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 57 GAAGA GAAGA(8/7)
MspA1I 40 CCGCTG C[AC]G^C[GT]G
MwoI 8 GCAAGATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 161 CCATGG C^CATGG
NlaIII 162 CATG CATG^
PflMI 95 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 188 AGGACCT [GA]G^G[AT]CC[CT]
SfaNI 80 GCATC GCATC(5/9)
TaqI 125 TCGA T^CGA
TseI 137 GCTGC G^C[AT]GC
TthHB8I 125 TCGA T^CGA
Van91I 95 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AvaII 157 GGACC G^G[AT]CC
189 GGACC
BsiYI 63 CCAACCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
95 CCATCAAGTGG
BslI 63 CCAACCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
95 CCATCAAGTGG
BssKI 84 CCTGG ^CC[ACGT]GG
174 CCTGG
BstF5I 81 CATCC GGATG(2/0)
53 GGATG
BstNI 84 CCTGG CC^[AT]GG
174 CCTGG
BstYI 11 AGATCT [GA]^GATC[CT]
59 AGATCC
Cfr13I 157 GGACC G^G[ACGT]CC
189 GGACC
DpnII 12 GATC ^GATC
60 GATC
EcoRII 84 CCTGG ^CC[AT]GG
174 CCTGG
EcoT14I 68 CCAAGG C^C[AT][AT]GG
161 CCATGG
FokI 81 CATCC GGATG(9/13)
53 GGATG
MboI 12 GATC ^GATC
60 GATC
MflI 11 AGATCT [GA]^GATC[CT]
59 AGATCC
MnlI 120 CCTC CCTC(7/6)
166 GAGG
MvaI 84 CCTGG CC^[AT]GG
174 CCTGG
NdeII 12 GATC ^GATC
60 GATC
NlaIV 157 GGACCC GG[ACGT]^[ACGT]CC
168 GGAGCC
Sau3AI 12 GATC ^GATC
60 GATC
Sau96I 157 GGACC G^G[ACGT]CC
189 GGACC
ScrFI 84 CCTGG CC^[ACGT]GG
174 CCTGG
StyI 68 CCAAGG C^C[AT][AT]GG
161 CCATGG
XhoII 11 AGATCT [GA]^GATC[CT]
59 AGATCC
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# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
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BsaJI HphI