Restriction map for
pFN21AE5362
##################################
# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiHKAI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 120 GACGCC G[GA]^CG[CT]C
Aor51HI 267 AGCGCT AGC^GCT
AvaII 27 GGACC G^G[AT]CC
BbsI 779 GAAGAC GAAGAC(2/6)
BciVI 599 GGATAC GTATCC(6/5)
BfaI 328 CTAG C^TAG
BmyI 635 GTGCCC G[AGT]GC[ACT]^C
BpuAI 779 GAAGAC GAAGAC(2/6)
BsaHI 120 GACGCC G[GA]^CG[CT]C
BsiWI 17 CGTACG C^GTACG
Bsp1286I 635 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 392 TGTACA T^GTACA
BspMI 178 GCAGGT ACCTGC(4/8)
BsrGI 392 TGTACA T^GTACA
BssSI 259 CTCGTG CACGAG(-5/-1)
CfoI 268 GCGC GCG^C
Cfr13I 27 GGACC G^G[ACGT]CC
DdeI 167 CTCAG C^T[ACGT]AG
DraI 313 TTTAAA TTT^AAA
Eco47III 267 AGCGCT AGC^GCT
FauI 131 GCGGG CCCGC(4/6)
HaeII 267 AGCGCT [GA]GCGC^[CT]
HhaI 268 GCGC GCG^C
Hin1I 120 GACGCC G[GA]^CG[CT]C
HinP1I 268 GCGC G^CGC
HincII 404 GTCAAC GT[CT]^[GA]AC
HindII 404 GTCAAC GT[CT]^[GA]AC
HphI 208 TCACC GGTGA(8/7)
MaeI 328 CTAG C^TAG
MaeIII 367 GTGAC ^GT[ACGT]AC
MseI 314 TTAA T^TAA
NlaIII 220 CATG CATG^
PleI 230 GACTC GAGTC(4/5)
PstI 377 CTGCAG CTGCA^G
Sau96I 27 GGACC G^G[ACGT]CC
SfaNI 306 GATGC GCATC(5/9)
SfcI 377 CTGCAG C^T[GA][CT]AG
SmaI 125 CCCGGG CCC^GGG
SmlI 58 CTCAAG C^T[CT][GA]AG
SspBI 392 TGTACA T^GTACA
Tru9I 314 TTAA T^TAA
Tsp45I 367 GTGAC ^GT[GC]AC
TspRI 527 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 125 CCCGGG C^CCGGG
XmaI 125 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 277 AAATTT [GA]^AATT[CT]
334 GAATTT
AlwI 466 GATCC GGATC(4/5)
440 GGATC
ApoI 277 AAATTT [GA]^AATT[CT]
334 GAATTT
AvaI 8 CTCGGG C^[CT]CG[GA]G
125 CCCGGG
BbvI 652 GCAGC GCAGC(8/12)
376 GCTGC
BseRI 22 GAGGAG GAGGAG(10/8)
67 GAGGAG
BsiEI 89 CGGCCG CG[GA][CT]^CG
153 CGGCCG
BsmAI 13 GAGAC GTCTC(1/5)
56 GTCTC
BsmBI 55 CGTCTC CGTCTC(1/5)
13 GAGACG
BsmFI 283 GGGAC GGGAC(10/14)
755 GGGAC
BsoBI 8 CTCGGG C^[CT]CG[GA]G
125 CCCGGG
BsrDI 396 CATTGC GCAATG(2/0)
607 GCAATG
BstF5I 531 GGATG GGATG(2/0)
682 GGATG
BstNI 45 CCAGG CC^[AT]GG
451 CCAGG
EagI 89 CGGCCG C^GGCCG
153 CGGCCG
EclXI 89 CGGCCG C^GGCCG
153 CGGCCG
Eco52I 89 CGGCCG C^GGCCG
153 CGGCCG
EcoRII 45 CCAGG ^CC[AT]GG
451 CCAGG
FokI 531 GGATG GGATG(9/13)
682 GGATG
HgaI 120 GACGC GACGC(5/10)
54 GCGTC
MaeII 16 ACGT A^CGT
573 ACGT
MboII 779 GAAGA GAAGA(8/7)
489 TCTTC
MvaI 45 CCAGG CC^[AT]GG
451 CCAGG
TaqI 80 TCGA T^CGA
225 TCGA
TseI 376 GCTGC G^C[AT]GC
652 GCAGC
TthHB8I 80 TCGA T^CGA
225 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AfaI AluI BcnI BsaJI
BsiYI BslI BssKI DpnII EaeI
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MboI MnlI MspI MwoI
NciI NdeII RsaI Sau3AI ScrFI
TfiI Tsp509I