Restriction map for
pFN21AE5662
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI Alw44I ApaLI
ApoI AscI AseI AsnI Asp718I
AvrII BamHI BbeI BbrPI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
EagI EclXI Eco52I EcoRI EcoRV
EcoT22I FbaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SmlI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 293 TCCGGA T^CCGGA
AflIII 910 ACACGT A^C[GA][CT]GT
AhdI 1494 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 443 CCTCAGG CC^T[ACGT]AGG
Aor51HI 737 AGCGCT AGC^GCT
Asp700I 1377 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1494 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 790 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 419 TGCGCA TGC^GCA
BbvCI 709 GCTGAGG CCTCAGC(-5/-2)
BcgI 858 GCAGGAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 998 GCCTCAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 293 TCCGGA T^CCGGA
BspEI 293 TCCGGA T^CCGGA
BsrBI 1042 GAGCGG CCGCTC(-3/-3)
BstEII 1326 GGTGACC G^GT[ACGT]ACC
BstPI 1326 GGTGACC G^GT[ACGT]ACC
BstYI 1013 AGATCC [GA]^GATC[CT]
Bsu36I 443 CCTCAGG CC^T[ACGT]AGG
Eam1105I 1494 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 189 GAAGAG CTCTTC(1/4)
Eco47III 737 AGCGCT AGC^GCT
Eco81I 443 CCTCAGG CC^T[ACGT]AGG
EcoNI 1152 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1326 GGTGACC G^GT[ACGT]ACC
EcoT14I 509 CCATGG C^C[AT][AT]GG
FseI 1338 GGCCGGCC GGCCGG^CC
FspI 419 TGCGCA TGC^GCA
HaeII 737 AGCGCT [GA]GCGC^[CT]
Ksp632I 189 GAAGAG CTCTTC(1/4)
MflI 1013 AGATCC [GA]^GATC[CT]
MroI 293 TCCGGA T^CCGGA
NaeI 1339 GCCGGC GCC^GGC
NcoI 509 CCATGG C^CATGG
NgoMIV 1339 GCCGGC G^CCGGC
NspI 1175 ACATGC [GA]CATG^[CT]
PflFI 790 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1447 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 62 AGTACT AGT^ACT
SexAI 791 ACCTGGT A^CC[AT]GGT
SfaNI 819 GATGC GCATC(5/9)
SnaBI 650 TACGTA TAC^GTA
StyI 509 CCATGG C^C[AT][AT]GG
Tth111I 790 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1389 CCACACAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1013 AGATCC [GA]^GATC[CT]
XmnI 1377 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 438 CGCG CG^CG
787 CGCG
AvaI 895 CCCGAG C^[CT]CG[GA]G
1057 CCCGAG
BalI 162 TGGCCA TGG^CCA
1278 TGGCCA
BanI 621 GGCACC G^G[CT][GA]CC
873 GGTGCC
BsaAI 650 TACGTA [CT]AC^GT[GA]
911 CACGTA
BsaWI 293 TCCGGA [AT]^CCGG[AT]
1203 ACCGGA
BseRI 10 GAGGAG GAGGAG(10/8)
192 GAGGAG
BsmAI 80 GAGAC GTCTC(1/5)
1492 GAGAC
BsmBI 80 GAGACG CGTCTC(1/5)
1492 GAGACG
BsoBI 895 CCCGAG C^[CT]CG[GA]G
1057 CCCGAG
BsrFI 644 ACCGGC [GA]^CCGG[CT]
1339 GCCGGC
BstUI 438 CGCG CG^CG
787 CGCG
BstXI 599 CCATGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1127 CCACGTTTGTGG
Cfr10I 644 ACCGGC [GA]^CCGG[CT]
1339 GCCGGC
DrdI 85 GACCTCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
844 GACCATTACGTC
DsaI 509 CCATGG C^C[GA][CT]GG
1334 CCGTGG
HgaI 436 GACGC GACGC(5/10)
1293 GACGC
HinfI 681 GACTC G^A[ACGT]TC
730 GAGTC
MluNI 162 TGGCCA TGG^CCA
1278 TGGCCA
MscI 162 TGGCCA TGG^CCA
1278 TGGCCA
MvnI 438 CGCG CG^CG
787 CGCG
PleI 681 GACTC GAGTC(4/5)
730 GAGTC
PpuMI 131 AGGACCT [GA]G^G[AT]CC[CT]
371 AGGACCT
PstI 340 CTGCAG CTGCA^G
1372 CTGCAG
PvuII 52 CAGCTG CAG^CTG
1487 CAGCTG
SfcI 340 CTGCAG C^T[GA][CT]AG
1372 CTGCAG
StuI 200 AGGCCT AGG^CCT
1529 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaII BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TaqI
TseI Tsp45I TspRI TthHB8I