Restriction map for
pFN21AE5353
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
AspEI AspI AviII AvrII BalI
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaI BsaWI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI HinP1I HphI
KasI MaeII MfeI MluI MluNI
MroI MscI MslI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NsiI NspI NspV PacI PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuII
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tsp45I Tth111I Van91I
XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 840 GGTACC G^GTACC
AcyI 238 GACGCC G[GA]^CG[CT]C
AlwNI 848 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 691 ATTAAT AT^TAAT
AsnI 691 ATTAAT AT^TAAT
Asp718I 840 GGTACC G^GTACC
AvaII 342 GGACC G^G[AT]CC
BamHI 273 GGATCC G^GATCC
BanI 840 GGTACC G^G[CT][GA]CC
BanII 145 GAGCTC G[GA]GC[CT]^C
BbvCI 255 CCTCAGC CCTCAGC(-5/-2)
BpmI 854 CTGGAG CTGGAG(16/14)
BsaBI 274 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 238 GACGCC G[GA]^CG[CT]C
BsgI 862 GTGCAG GTGCAG(16/14)
BsiEI 643 CGATCG CG[GA][CT]^CG
BsmFI 341 GGGAC GGGAC(10/14)
BsrDI 165 GCAATG GCAATG(2/0)
BsrFI 344 ACCGGC [GA]^CCGG[CT]
BstAPI 603 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 260 GCACGC GC[ACGT]^[ACGT]GC
Cfr10I 344 ACCGGC [GA]^CCGG[CT]
DraI 420 TTTAAA TTT^AAA
DraII 849 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoNI 483 CCTCAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 849 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 835 CCATGG C^C[AT][AT]GG
HaeIII 850 GGCC GG^CC
HgaI 238 GACGC GACGC(5/10)
Hin1I 238 GACGCC G[GA]^CG[CT]C
HincII 157 GTTAAC GT[CT]^[GA]AC
HindII 157 GTTAAC GT[CT]^[GA]AC
HindIII 740 AAGCTT A^AGCTT
HinfI 251 GATTC G^A[ACGT]TC
HpaI 157 GTTAAC GTT^AAC
KpnI 840 GGTACC GGTAC^C
KspI 242 CCGCGG CCGC^GG
MaeIII 803 GTAAC ^GT[ACGT]AC
MamI 274 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 835 CCATGG C^CATGG
NruI 327 TCGCGA TCG^CGA
PaeR7I 551 CTCGAG C^TCGAG
PshBI 691 ATTAAT AT^TAAT
PstI 349 CTGCAG CTGCA^G
PvuI 643 CGATCG CGAT^CG
SacI 145 GAGCTC GAGCT^C
SacII 242 CCGCGG CCGC^GG
SapI 493 GCTCTTC GCTCTTC(1/4)
SmaI 338 CCCGGG CCC^GGG
SmlI 551 CTCGAG C^T[CT][GA]AG
StyI 835 CCATGG C^C[AT][AT]GG
TfiI 251 GATTC G^A[AT]TC
TspRI 895 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 25 TCTAGA T^CTAGA
XcmI 886 CCAGACTATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 551 CTCGAG C^TCGAG
XmaCI 338 CCCGGG C^CCGGG
XmaI 338 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AspHI 145 GAGCTC G[AT]GC[AT]^C
877 GAGCAC
AvaI 338 CCCGGG C^[CT]CG[GA]G
551 CTCGAG
BbsI 225 GAAGAC GAAGAC(2/6)
625 GAAGAC
BcnI 338 CCCGG CC^[GC]GG
339 CCGGG
BfaI 26 CTAG C^TAG
766 CTAG
BpuAI 225 GAAGAC GAAGAC(2/6)
625 GAAGAC
BsiHKAI 145 GAGCTC G[AT]GC[AT]^C
877 GAGCAC
BsmAI 657 GAGAC GTCTC(1/5)
549 GTCTC
BsoBI 338 CCCGGG C^[CT]CG[GA]G
551 CTCGAG
Bsp1407I 12 TGTACA T^GTACA
927 TGTACA
BspMI 824 ACCTGC ACCTGC(4/8)
606 GCAGGT
BsrGI 12 TGTACA T^GTACA
927 TGTACA
BsrI 234 ACTGG ACTGG(1/-1)
711 CCAGT
BstF5I 970 CATCC GGATG(2/0)
501 GGATG
BstNI 150 CCTGG CC^[AT]GG
853 CCTGG
BstYI 273 GGATCC [GA]^GATC[CT]
831 AGATCC
Cfr13I 342 GGACC G^G[ACGT]CC
850 GGCCC
DdeI 256 CTCAG C^T[ACGT]AG
954 CTCAG
DsaI 242 CCGCGG C^C[GA][CT]GG
835 CCATGG
EarI 494 CTCTTC CTCTTC(1/4)
406 GAAGAG
EcoRII 150 CCTGG ^CC[AT]GG
853 CCTGG
FauI 264 GCGGG CCCGC(4/6)
354 GCGGG
FokI 970 CATCC GGATG(9/13)
501 GGATG
HapII 339 CCGG C^CGG
345 CCGG
HpaII 339 CCGG C^CGG
345 CCGG
Ksp632I 494 CTCTTC CTCTTC(1/4)
406 GAAGAG
MaeI 26 CTAG C^TAG
766 CTAG
MflI 273 GGATCC [GA]^GATC[CT]
831 AGATCC
MspA1I 242 CCGCGG C[AC]G^C[GT]G
352 CAGCGG
MspI 339 CCGG C^CGG
345 CCGG
MvaI 150 CCTGG CC^[AT]GG
853 CCTGG
NciI 338 CCCGG CC^[GC]GG
339 CCGGG
Sau96I 342 GGACC G^G[ACGT]CC
850 GGCCC
SfcI 349 CTGCAG C^T[GA][CT]AG
682 CTACAG
SspBI 12 TGTACA T^GTACA
927 TGTACA
TaqI 552 TCGA T^CGA
651 TCGA
TthHB8I 552 TCGA T^CGA
651 TCGA
XhoII 273 GGATCC [GA]^GATC[CT]
831 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI BbvI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BssKI
BstUI DpnII EcoRI Fnu4HI ItaI
MboI MboII MnlI MseI MvnI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI ScrFI SfaNI Tru9I TseI
Tsp509I