Restriction map for
pFN21AE6681
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmFI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrFI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI Hin1I HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
Van91I XcmI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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ApaI 297 GGGCCC GGGCC^C
Asp700I 613 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 211 GTGCTC G[AT]GC[AT]^C
AvaI 377 CCCGGG C^[CT]CG[GA]G
BanII 297 GGGCCC G[GA]GC[CT]^C
BbvCI 54 GCTGAGG CCTCAGC(-5/-2)
BglII 723 AGATCT A^GATCT
BseRI 608 GAGGAG GAGGAG(10/8)
BsgI 206 GTGCAG GTGCAG(16/14)
BsiHKAI 211 GTGCTC G[AT]GC[AT]^C
BsmBI 68 CGTCTC CGTCTC(1/5)
BsoBI 377 CCCGGG C^[CT]CG[GA]G
Bsp1407I 731 TGTACA T^GTACA
BspMI 807 GCAGGT ACCTGC(4/8)
BsrDI 517 GCAATG GCAATG(2/0)
BsrGI 731 TGTACA T^GTACA
BssHII 28 GCGCGC G^CGCGC
BssSI 773 CACGAG CACGAG(-5/-1)
BstXI 335 CCAGTGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 723 AGATCT [GA]^GATC[CT]
DraI 901 TTTAAA TTT^AAA
EcoNI 571 CCTCAAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 82 GATATC GAT^ATC
HgaI 67 GCGTC GACGC(5/10)
HincII 322 GTCAAC GT[CT]^[GA]AC
HindII 322 GTCAAC GT[CT]^[GA]AC
MaeII 9 ACGT A^CGT
MflI 723 AGATCT [GA]^GATC[CT]
MspA1I 77 CAGCTG C[AC]G^C[GT]G
NcoI 788 CCATGG C^CATGG
NspI 959 ACATGC [GA]CATG^[CT]
PleI 320 GAGTC GAGTC(4/5)
PpuMI 380 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 77 CAGCTG CAG^CTG
SmaI 377 CCCGGG CCC^GGG
SmlI 572 CTCAAG C^T[CT][GA]AG
SspBI 731 TGTACA T^GTACA
Tsp45I 771 GTCAC ^GT[GC]AC
XbaI 588 TCTAGA T^CTAGA
XhoII 723 AGATCT [GA]^GATC[CT]
XmaCI 377 CCCGGG C^CCGGG
XmaI 377 CCCGGG C^CCGGG
XmnI 613 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 29 CGCG CG^CG
31 CGCG
AcsI 448 AAATTT [GA]^AATT[CT]
492 GAATTT
ApoI 448 AAATTT [GA]^AATT[CT]
492 GAATTT
AvaII 381 GGTCC G^G[AT]CC
837 GGTCC
BmyI 211 GTGCTC G[AGT]GC[ACT]^C
297 GGGCCC
Bsp1286I 211 GTGCTC G[AGT]GC[ACT]^C
297 GGGCCC
BstUI 29 CGCG CG^CG
31 CGCG
DsaI 788 CCATGG C^C[GA][CT]GG
833 CCGTGG
EcoT14I 788 CCATGG C^C[AT][AT]GG
942 CCAAGG
HaeII 3 GGCGCT [GA]GCGC^[CT]
48 GGCGCT
HphI 810 GGTGA GGTGA(8/7)
816 TCACC
MaeIII 771 GTCAC ^GT[ACGT]AC
991 GTTAC
MvnI 29 CGCG CG^CG
31 CGCG
NlaIII 789 CATG CATG^
960 CATG
SfaNI 399 GATGC GCATC(5/9)
592 GATGC
StyI 788 CCATGG C^C[AT][AT]GG
942 CCAAGG
TfiI 183 GATTC G^A[AT]TC
813 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI BbsI BbvI
BcnI BfaI BpuAI BsaI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MboI MboII MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIV
RsaI Sau3AI Sau96I ScrFI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I