Restriction map for
pFN21AE5545
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BsaBI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI Bst1107I BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeIII MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SapI ScaI
SfoI SgrAI SmlI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TfiI Tsp45I Tsp509I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AcyI 253 GACGCC G[GA]^CG[CT]C
AflIII 326 ACACGT A^C[GA][CT]GT
AlwI 534 GGATC GGATC(4/5)
AocI 234 CCTGAGG CC^T[ACGT]AGG
ApaI 54 GGGCCC GGGCC^C
AspHI 1135 GAGCTC G[AT]GC[AT]^C
AviII 833 TGCGCA TGC^GCA
BanI 1041 GGTGCC G^G[CT][GA]CC
BbsI 667 GTCTTC GAAGAC(2/6)
BfaI 19 CTAG C^TAG
BglII 320 AGATCT A^GATCT
BpuAI 667 GTCTTC GAAGAC(2/6)
BsaAI 559 TACGTG [CT]AC^GT[GA]
BsaHI 253 GACGCC G[GA]^CG[CT]C
BsaI 571 GAGACC GGTCTC(1/5)
BsaWI 422 ACCGGA [AT]^CCGG[AT]
BsgI 400 CTGCAC GTGCAG(16/14)
BsiHKAI 1135 GAGCTC G[AT]GC[AT]^C
BsmAI 571 GAGAC GTCTC(1/5)
BspHI 314 TCATGA T^CATGA
BspMI 398 ACCTGC ACCTGC(4/8)
BsrDI 372 CATTGC GCAATG(2/0)
BssHII 639 GCGCGC G^CGCGC
BstBI 697 TTCGAA TT^CGAA
BstXI 377 CCAAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 320 AGATCT [GA]^GATC[CT]
Bsu36I 234 CCTGAGG CC^T[ACGT]AGG
DsaI 1100 CCGCGG C^C[GA][CT]GG
EaeI 302 TGGCCG [CT]^GGCC[GA]
Eco81I 234 CCTGAGG CC^T[ACGT]AGG
FspI 833 TGCGCA TGC^GCA
HaeII 659 AGCGCC [GA]GCGC^[CT]
Hin1I 253 GACGCC G[GA]^CG[CT]C
HinfI 1117 GACTC G^A[ACGT]TC
KspI 1100 CCGCGG CCGC^GG
MaeI 19 CTAG C^TAG
MflI 320 AGATCT [GA]^GATC[CT]
MseI 464 TTAA T^TAA
MslI 1074 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV 697 TTCGAA TT^CGAA
PflMI 591 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1117 GACTC GAGTC(4/5)
PvuII 616 CAGCTG CAG^CTG
RcaI 314 TCATGA T^CATGA
SacI 1135 GAGCTC GAGCT^C
SacII 1100 CCGCGG CCGC^GG
SbfI 885 CCTGCAGG CCTGCA^GG
SexAI 83 ACCAGGT A^CC[AT]GGT
SfaNI 260 GCATC GCATC(5/9)
SfiI 1098 GGCCGCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 697 TTCGAA TT^CGAA
SmaI 44 CCCGGG CCC^GGG
Sse8387I 885 CCTGCAGG CCTGCA^GG
Tru9I 464 TTAA T^TAA
Van91I 591 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 290 CCACCGACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 320 AGATCT [GA]^GATC[CT]
XmaCI 44 CCCGGG C^CCGGG
XmaI 44 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BglI 181 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1099 GCCGCGGGGGC
BlpI 564 GCTGAGC GC^T[ACGT]AGC
780 GCTGAGC
Bpu1102I 564 GCTGAGC GC^T[ACGT]AGC
780 GCTGAGC
BssSI 484 CACGAG CACGAG(-5/-1)
1065 CTCGTG
BstAPI 4 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
461 GCATTAACTGC
CelII 564 GCTGAGC GC^T[ACGT]AGC
780 GCTGAGC
EcoNI 234 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
882 CCTCCTGCAGG
EcoT14I 578 CCTTGG C^C[AT][AT]GG
797 CCAAGG
HgaI 253 GACGC GACGC(5/10)
665 GCGTC
PpuMI 478 GGGACCC [GA]G^G[AT]CC[CT]
542 AGGACCC
StyI 578 CCTTGG C^C[AT][AT]GG
797 CCAAGG
TaqI 698 TCGA T^CGA
1151 TCGA
TspRI 222 CACTG CA[GC]TG[ACGT][ACGT]^
299 CAGTG
TthHB8I 698 TCGA T^CGA
1151 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfcI TseI